Engineering Myocardium for Heart Regeneration—Advancements, Considerations, and Future Directions

Jarrell, Dillon K.; Vanderslice, Ethan J.; VeDepo, Mitchell C.; Jacot, Jeffrey G.

doi:10.3389/fcvm.2020.586261

Cited by 13 publications

(12 citation statements)

References 110 publications

(113 reference statements)

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“…There remains high interest in cardiac tissue engineering, which creates scaffolds to control cell behavior, thereby restoring the natural architecture and function of various parts of the heart. The concept of in situ tissue engineering represents an innovation for creating a living and immunocompatible scaffold, which significantly reduces the time of implant preparation [ 47 ]. In this line of research, the great advances in laboratory studies of iPSCs explain the steady progress in cardiac tissue engineering in recent decades, and are probably the next focus in cardiac regeneration.…”

Section: Discussionmentioning

confidence: 99%

Do Human iPSC-Derived Cardiomyocytes Cultured on PLA Scaffolds Induce Expression of CD28/CTLA-4 by T Lymphocytes?

Sergeevichev

Balashov

Козырева

et al. 2022

JFB

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Many research groups have developed various types of tissue-engineered cardiac constructs. However, the immunological properties of such artificial tissues are not yet fully understood. Previously, we developed microfiber scaffolds carrying human iPSC-derived cardiomyocytes (hiPSC-CM). In this work, we evaluated the ability of these tissue-engineered constructs to activate the expression of CD28 and CTLA-4 proteins on T lymphocytes, which are early markers of the immune response. For this purpose, electrospun PLA microfiber scaffolds were seeded with hiPSC-CM and cultured for 2 weeks. Allogeneic mononuclear cells were then co-cultured for 48 h with three groups of samples: bare scaffolds, pure cardiomyocyte culture and tissue-engineered constructs, followed by analysis of CD28/CTLA-4 expression on T lymphocytes using flow cytometry. PLA scaffolds and concanavalin A stimulation (positive control) statistically significantly increased CD28 expression on CD4+ T cells (up to 61.3% and 66.3%) CD8+ T cells (up to 17.8% and 21.7%). CD28/CTLA-4 expression was not increased when T lymphocytes were co-cultured with cardiac tissue-engineered constructs and iPSC-CM monolayers. Thus, iPSC-CM in monolayers and on PLA microfiber scaffolds did not induce T cell activation, which suggests that such cardiac constructs would not be a cause of rejection after implantation.

show abstract

Section: Discussionmentioning

confidence: 99%

Do Human iPSC-Derived Cardiomyocytes Cultured on PLA Scaffolds Induce Expression of CD28/CTLA-4 by T Lymphocytes?

Sergeevichev

Balashov

Козырева

et al. 2022

JFB

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show abstract

“…There remains a high interest in cardiac tissue engineering, which creates scaffolds to control cell behavior, thereby restoring the natural architecture and function of various parts of the heart. The concept of in situ tissue engineering represents an innovation for creating a living and immunocompatible scaffold, which significantly reduces the time of implant preparation [47]. In this line of research, the great advances in laboratory studies of iPSCs explain the steady progress in cardiac tissue engineering in recent decades and is probably the next focus in cardiac regeneration.…”

Section: Discussionmentioning

confidence: 99%

Do Human iPSC Derived Cardiomyocytes Cultured on PLA Scaffolds Induce Expression of CD28/CTLA 4 by T Lymphocytes?

Sergeevichev¹,

Balashov²,

Козырева³

et al. 2021

Preprint

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Different types of engineered cardiac constructs are being developed nowadays by many research groups. However, the immunological properties of such artificial tissues are not yet clearly understood. Previously, we have studied microfiber scaffolds carrying iPSC-derived cardiomyocytes. In this work, we evaluated the ability of these tissue-engineered constructs to activate the expression of CD28 and CTLA-4 proteins in T-lymphocytes which are early markers of the immune response. For this purpose electrospun PLA nanofibrous scaffolds were seeded with human iPSCs-CM and cultivated for 2 weeks. After, allogeneic mononuclear cells were co-cultured during 48 hours with 3 groups of samples that were tissue-engineered constructs, pure culture of cardiomyocytes and bare scaffolds followed by analysis of CD28/CTLA-4 expression on T-lymphocytes via flow cytometry. PLA scaffolds and concanavalin A (positive control) stimulation statistically significantly increased CD28 expression on CD4+ cells (up to 61.3% and 66.3%) and on CD8+ cells (up to 17.8% and 21.7%). CD28/CTLA-4 expression didn’t increase during co-cultivation of T-lymphocytes with cardiac engineered constructs and iPSC-CM monolayers. Thus, iPSCs-CM in monolayers and on PLA nanofibrous scaffolds didn’t cause T-cell activation, which allows us to expect that such cardiac constructs are not a cause of rejection after implantation.

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“…Since the beginning of mankind, people have been plagued by various diseases. Although cures for some diseases have been found thanks to the rapid development of modern medicine, chronic diseases such as heart disease, cerebrovascular disease and cancer are still the top killers of humans [1], [2]. If these diseases are detected in a timely manner or early in the incubation period, the patient survival time can be significantly prolonged after standardized treatment [3].…”

Section: Introductionmentioning

confidence: 99%

An Adaptive Energy Efficient MAC Protocol for RF Energy Harvesting WBANs

et al. 2023

IEEE Trans. Commun.

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Continuous and remote health monitoring medical applications with heterogeneous requirements can be realized through wireless body area networks (WBANs). Energy harvesting is adopted to enable low-power health applications and longterm monitoring without battery replacement, which have drawn significant interest recently. Because energy harvesting WBANs are obviously different from battery-powered ones, network protocols should be designed accordingly to improve network performance. In this article, an efficient cross-layer media access control protocol is proposed for radio frequency powered energy harvesting WBANs. We redesigned the superframe structure, which can be rescheduled by the coordinator dynamically. A time switching (TS) strategy is used when sensors harvest energy from radio frequency signals broadcast by the coordinator, and a transmission power adjustment scheme is proposed for sensors based on the energy harvesting efficiency and the network environment. Energy efficiency can be effectively improved that more packets can be uploaded using limited energy. The length of the energy harvesting period is determined by the coordinator to balance the channel resources and energy requirements of sensors and further improve the network performance. Numerical simulation results show that our protocol can provide superior system performance for long-term periodic health monitoring applications.

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Engineering Myocardium for Heart Regeneration—Advancements, Considerations, and Future Directions

Cited by 13 publications

References 110 publications

Do Human iPSC-Derived Cardiomyocytes Cultured on PLA Scaffolds Induce Expression of CD28/CTLA-4 by T Lymphocytes?

Do Human iPSC-Derived Cardiomyocytes Cultured on PLA Scaffolds Induce Expression of CD28/CTLA-4 by T Lymphocytes?

Do Human iPSC Derived Cardiomyocytes Cultured on PLA Scaffolds Induce Expression of CD28/CTLA 4 by T Lymphocytes?

An Adaptive Energy Efficient MAC Protocol for RF Energy Harvesting WBANs

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