2020
DOI: 10.1016/j.bej.2020.107739
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Engineering mycobacteria artificial promoters and ribosomal binding sites for enhanced sterol production

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Cited by 17 publications
(15 citation statements)
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“…The RBS sequence is a key factor in determining the translation initiation rate and protein expression level, and optimizing this region is considered an effective strategy to increase the yield of recombinant protein [ 17 , 41 , 42 , 43 ]. In order to better control the translation initiation rate and protein expression levels, the RBS calculator was developed and applied in the regulation of gene expression of recombinant strains [ 33 ].…”
Section: Resultsmentioning
confidence: 99%
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“…The RBS sequence is a key factor in determining the translation initiation rate and protein expression level, and optimizing this region is considered an effective strategy to increase the yield of recombinant protein [ 17 , 41 , 42 , 43 ]. In order to better control the translation initiation rate and protein expression levels, the RBS calculator was developed and applied in the regulation of gene expression of recombinant strains [ 33 ].…”
Section: Resultsmentioning
confidence: 99%
“…In order to better control the translation initiation rate and protein expression levels, the RBS calculator was developed and applied in the regulation of gene expression of recombinant strains [ 33 ]. Previous studies have used the RBS calculator to generate the RBS sequence library, the purpose of which was to optimize protein expression levels; consequently, the expression levels of proteins have been significantly improved [ 17 , 40 ].…”
Section: Resultsmentioning
confidence: 99%
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“…For example, while combining multiple binding sites into one promoter has been shown to lead to increased promoter strength, as reviewed by Blazeck and Alper (2013), (Wang et al (2019a) report that introduction of multiple r-factor binding sites into one promoter can lead to decreased expression. Similarly, Sun et al (2020a) found that combining multiple strong promoters in tandem did not lead to changes in expression levels. Despite the limitations, the various methods outlined above are valuable approaches that enable the generation of promoter variants with various characteristics.…”
Section: Hybrid Mutated and (Semi-)artificial Promotersmentioning
confidence: 97%
“…Similarly, Sun et al . ( 2020a ) found that combining multiple strong promoters in tandem did not lead to changes in expression levels. Despite the limitations, the various methods outlined above are valuable approaches that enable the generation of promoter variants with various characteristics.…”
Section: ′ Regulatory Sequencesmentioning
confidence: 99%