Engineering model proteins for Photosystem II function

Wydrzynski, Tom; Hillier, Warwick; Conlan, Brendon

doi:10.1007/s11120-007-9271-0

Cited by 26 publications

(13 citation statements)

References 76 publications

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“…In this context, the role of water and solvation in the imaging process (also with regard to the so-called Guckenberger experiment [17]) is examined and consequences for solar fuel generation at enzyme-semiconductor junctions are outlined. Although the inorganic absorber-enzyme system should be analyzed in detail with regard to possible application in catalytic in solar fuel production, alternatives for the realization of artificial photosynthesis [18][19][20], using nanodimensioned inorganic/organic absorbers that assume the role of the light harvesting complexes [21] which transfer energy to enzymes or their synthetic substitutes should also be considered. With regard to the charge transfer processes, electron transfer is compared to single molecule and multichromic Förster transfer [22,23] and Dexter exciton hopping [24].…”

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confidence: 99%

Enzyme–semiconductor interactions: Routes from fundamental aspects to photoactive devices

Lewerenz

2008

Physica Status Solidi (b)

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Scanning tunnelling microscopy (STM) experiments at protein–semiconductor systems are analyzed using concepts from applied semiconductor physics such as Fermi level pinning and MIS (metal–insulator–semiconductor) junction electronics. Routes for immobilization of enzymes (proteins) on nanostructured surfaces of MoTe2 and Si are outlined using so‐called DLVO and non‐DLVO interaction forces. An overview of the catalytic activity of the imaged enzymes, reverse transcriptases of the retroviruses HIV 1 and AMV (avian myeloblastosis virus), is given including their tertiary structural properties which is revealed also in the STM and tapping mode AFM images. For the interpretation of STM images, a resonant charge transfer mechanism is invoked, based on the potential dependence of the image contrast and the energy band structure of MoTe2 near the valence band maximum. First analyses of the charge transport from the semiconductor to the STM tip at negative bias of MoTe2 suggest that the observed uninhibited conductivity in the constant current experiments results from solvation‐assisted release of electrons from traps that exist along the polypeptide chains and that charge transport occurs at the circumference of the enzymes where biological water is present. Therefore, charge injection into catalytically active enzymes such as hydrogenase or water oxidase of Photosystem I and II with subsequent charge transport to the active sites appears difficult to realize. Possibilities of radiation‐less long‐distance energy transfer based on the Förster mechanism, its multichromic extension and on Dexter exciton hopping are considered for catalytically active hybrid inorganic/organic absorber–enzyme structures. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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confidence: 99%

Enzyme–semiconductor interactions: Routes from fundamental aspects to photoactive devices

Lewerenz

2008

Physica Status Solidi (b)

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“…Designed proteins also do not give rise to native-like structures which make the task even more difficult. Because of this, semiartificial systems where a natural protein is modified to incorporate a chain of cofactors including the photocatalyst have become a more attractive approach (for example see Wydrzynski et al 2007). This approach is assisted by the existence of structural data for these proteins and the blueprint provided by newly resolved structures of PSI and PSII proteins.…”

Section: Resultsmentioning

confidence: 99%

Artificial photoactive proteins

Razeghifard

2008

Photosynth Res

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Solar power is the most abundant source of renewable energy. In this respect, the goal of making photoactive proteins is to utilize this energy to generate an electron flow. Photosystems have provided the blueprint for making such systems, since they are capable of converting the energy of light into an electron flow using a series of redox cofactors. Protein tunes the redox potential of the cofactors and arranges them such that their distance and orientation are optimal for the creation of a stable charge separation. The aim of this review is to present an overview of the literature with regard to some elegant functional structures that protein designers have created by introducing cofactors and photoactivity into synthetic proteins.

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“…[35] Rational design, however, involves an engineering approach to protein design, often in combination with molecular modelling of the protein structure. The approach leads itself to redesigning existing proteins for a change (or improvement) of function [30,36] or to approach this with a fresh palette and to create de novo proteins with novel protein sequences. [37][38][39][40] The present authors' interest lies in engineering proteins through rational design to create nature-inspired photocatalytic proteins for artificial photosynthesis.…”

Section: Engineering Proteins For Artificial Photosynthesismentioning

confidence: 99%

“…[93,111] In the alternative approach of modifying natural proteins, the advantages lie in the remarkable inherent features of selected proteins that may facilitate redesigning with minimal modification. [36] Another highly valuable attribute of manipulating naturally occurring proteins is the ease of incorporating the modified variants into biological systems through recombinant technology, and therefore the possibility of immediate in vivo characterisation.…”

Section: Modified Bacterioferritin As a Photoactive Reaction Centrementioning

confidence: 99%

Perspectives for Photobiology in Molecular Solar Fuels

Hingorani

Hillier

2012

Aust. J. Chem.

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This paper presents an overview of the prospects for bio-solar energy conversion. The Global Artificial Photosynthesis meeting at Lord Howe Island (14–18 August 2011) underscored the dependence that the world has placed on non-renewable energy supplies, particularly for transport fuels, and highlighted the potential of solar energy. Biology has used solar energy for free energy gain to drive chemical reactions for billions of years. The principal conduits for energy conversion on earth are photosynthetic reaction centres – but can they be harnessed, copied and emulated? In this communication, we initially discuss algal-based biofuels before investigating bio-inspired solar energy conversion in artificial and engineered systems. We show that the basic design and engineering principles for assembling photocatalytic proteins can be used to assemble nanocatalysts for solar fuel production.

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Engineering model proteins for Photosystem II function

Cited by 26 publications

References 76 publications

Enzyme–semiconductor interactions: Routes from fundamental aspects to photoactive devices

Enzyme–semiconductor interactions: Routes from fundamental aspects to photoactive devices

Artificial photoactive proteins

Perspectives for Photobiology in Molecular Solar Fuels

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