2023
DOI: 10.1101/2023.10.31.565021
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Engineering microvascular networks using a KLF2 reporter to probe flow-dependent endothelial cell function

Adriana Blazeski,
Marie A. Floryan,
Oscar R. Fajardo-Ramírez
et al.

Abstract: Shear stress generated by the flow of blood in the vasculature is a potent regulator of endothelial cell phenotype and vascular structure. While vascular responses to flow are complex and context-dependent, endothelial cell signaling in response to shear stress induced by laminar flows is coordinated by the transcription factor KLF2. The expression of KLF2 in endothelial cells is associated with a quiescent, anti-inflammatory phenotype and has been well characterized in two-dimensional systems, but has not bee… Show more

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Cited by 1 publication
(2 citation statements)
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“…Specifically, we used ECs carrying a flow-inducible reporter of KLF2 transcription [123] to construct threedimensional human MVNs within microfluidic devices and applied flow (or not) using an on-chip microfluidic pump, as in [124] (see also [125,126]).…”
Section: Engineered Microvascular Network (Mvns) Made From Human Ecs ...mentioning
confidence: 99%
See 1 more Smart Citation
“…Specifically, we used ECs carrying a flow-inducible reporter of KLF2 transcription [123] to construct threedimensional human MVNs within microfluidic devices and applied flow (or not) using an on-chip microfluidic pump, as in [124] (see also [125,126]).…”
Section: Engineered Microvascular Network (Mvns) Made From Human Ecs ...mentioning
confidence: 99%
“…We did not expose MVNs cultured under static conditions to flow. We provide further details about these protocols in [124]. See also [125,126].…”
Section: Engineered Microvascular Network (Mvns) Made From Human Ecs ...mentioning
confidence: 99%