Engineering long shelf life multi-layer biologically active surfaces on microfluidic devices for point of care applications

Asghar, Waseem; Yüksekkaya, Mehmet Emin; Shafiee, Hadi; Zhang, Michael; Özen, Mehmet Özgün; İnci, Fatih; Kocakulak, Mustafa; Demirci, Utkan

doi:10.1038/srep21163

Cited by 45 publications

(58 citation statements)

References 66 publications

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“…The CO 2 laser cutter has been widely used to cut the paper, PMMA, and double-sided adhesive (DSA) to fabricate microfluidic devices [2,3,16,49–53]. A simple and inexpensive method was presented for the fabrication of microfluidic devices (Figure 4(a)) [3].…”

Section: Fabrication Techniques Of μPadsmentioning

confidence: 99%

“…Around 5.8 billion people worldwide live in settings categorized as low and middle income [1] and do not have access to the expensive medical facilities required for current immunoassays and other disease detection technologies [2,3]. Therefore, it is very important to develop inexpensive and portable point-of-care (POC) devices that can provide rapid and accurate results.…”

Section: Introductionmentioning

confidence: 99%

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Paper-based analytical devices for clinical diagnosis: recent advances in the fabrication techniques and sensing mechanisms

Sher

Zhuang²,

Demirci³

et al. 2017

Expert Review of Molecular Diagnostics

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211

157

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Introduction There is a significant interest in developing inexpensive portable biosensing platforms for various applications including disease diagnostics, environmental monitoring, food safety, and water testing at the point-of-care (POC) settings. Current diagnostic assays available in the developed world require sophisticated laboratory infrastructure and expensive reagents. Hence, they are not suitable for resource-constrained settings with limited financial resources, basic health infrastructure, and few trained technicians. Cellulose and flexible transparency paper-based analytical devices have demonstrated enormous potential for developing robust, inexpensive and portable devices for disease diagnostics. These devices offer promising solutions to disease management in resource-constrained settings where the vast majority of the population cannot afford expensive and highly sophisticated treatment options. Areas covered In this review, the authors describe currently developed cellulose and flexible transparency paper-based microfluidic devices, device fabrication techniques, and sensing technologies that are integrated with these devices. The authors also discuss the limitations and challenges associated with these devices and their potential in clinical settings. Expert commentary In recent years, cellulose and flexible transparency paper-based microfluidic devices have demonstrated the potential to become future healthcare options despite a few limitations such as low sensitivity and reproducibility.

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Section: Fabrication Techniques Of μPadsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Paper-based analytical devices for clinical diagnosis: recent advances in the fabrication techniques and sensing mechanisms

Sher

Zhuang²,

Demirci³

et al. 2017

Expert Review of Molecular Diagnostics

Self Cite

211

157

View full text Add to dashboard Cite

show abstract

“…[38,127–146, 164] Microfluidic LOC devices enhance early detection and identification of fungi in the blood stream. This technology allows for a device to be used in resource-constrained settings where there are no centralized laboratories available for implementing conventional culturing techniques.…”

Section: Nonconventional Detection For Candida: Lab Chip (Loc) Devicementioning

confidence: 99%

Advances inCandidadetection platforms for clinical and point-of-care applications

Safavieh

Coarsey

Esiobu

et al. 2016

Critical Reviews in Biotechnology

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Invasive candidiasis remains one of the most serious community and healthcare-acquired infections worldwide. Conventional Candida detection methods based on blood and plate culture are time-consuming and require at least 2–4 days to identify various Candida species. Despite considerable advances for candidiasis detection, the development of simple, compact and portable point-of-care diagnostics for rapid and precise testing that automatically performs cell lysis, nucleic acid extraction, purification and detection still remains a challenge. Here, we systematically review most prominent conventional and nonconventional techniques for the detection of various Candida species, including Candida staining, blood culture, serological testing and nucleic acid-based analysis. We also discuss the most advanced lab on a chip devices for candida detection.

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“…18 One of the main drivers for these POC technologies is the detection of diseases in resource-limited countries. 19–25 For example, commercial POC kits have been recently developed to detect human immunodeficiency virus (HIV) and tuberculosis in such settings. 26 However, there are significant logistical, technical, and social barriers that need to be overcome when performing testing at these sites, and many of these technologies still require the recruitment and training of personnel (Fig.…”

Section: Introductionmentioning

confidence: 99%

Photonic crystals: emerging biosensors and their promise for point-of-care applications

et al. 2017

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Biosensors are extensively employed for diagnosing a broad array of diseases and disorders in clinical settings worldwide. The implementation of biosensors at the point-of-care (POC), such as at primary clinics or the bedside, faces impediments because they may require highly trained personnel, have long assay times, large sizes, and high instrumental cost. Thus, there exists a need to develop inexpensive, reliable, user-friendly, and compact biosensing systems at the POC. Biosensors incorporated with photonic crystal (PC) structures hold promise to address many of the aforementioned challenges facing the development of new POC diagnostics. Currently, PC-based biosensors have been employed for detecting a variety of biotargets, such as cells, pathogens, proteins, antibodies, and nucleic acids, with high efficiency and selectivity. In this review, we provide a broad overview of PCs by explaining their structures, fabrication techniques, and sensing principles. Furthermore, we discuss recent applications of PC-based biosensors incorporated with emerging technologies, including telemedicine, flexible and wearable sensing, smart materials and metamaterials. Finally, we discuss current challenges associated with existing biosensors, and provide an outlook for PC-based biosensors and their promise at the POC.

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Engineering long shelf life multi-layer biologically active surfaces on microfluidic devices for point of care applications

Cited by 45 publications

References 66 publications

Paper-based analytical devices for clinical diagnosis: recent advances in the fabrication techniques and sensing mechanisms

Paper-based analytical devices for clinical diagnosis: recent advances in the fabrication techniques and sensing mechanisms

Advances inCandidadetection platforms for clinical and point-of-care applications

Photonic crystals: emerging biosensors and their promise for point-of-care applications

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