Engineering lipase B from Candida antarctica

Biocatalytic preparation of enantioenriched 3,4-dihydroxypiperidines and theoretical study of Candida antarctica lipase B enantioselectivity

“…Since the X-ray crystal structure of CAL-B was resolved, 15 several molecular modelling 16 and molecular dynamics studies 17 have been made in order to explain its excellent reactivity and selectivity with chiral alcohols and amines. These reports have been very useful to make rational design of this biocatalyst 18 with improved selectivities, 17b,19 or catalyzing through novel reaction pathways. 20 Thus, engineered CAL-B has been utilized to perform aldol, 21 Michael-type additions, 22 or Baeyer-Villiger reactions.…”

Understanding the Plasticity of the α/β Hydrolase Fold: Lid Swapping on the Candida antarctica Lipase B Results in Chimeras with Interesting Biocatalytic Properties

“…[13,14] More recently, other groups have also generated and reported CALB variants with improved properties. [15] A group from Schering-Plough used error-prone PCR to produce mutants with increased thermostability [16] and DNA family shuffling to produce other mutants with increased hydrolysis activity toward a prochiral diester substrate. [17] In the latter work homologous lipases from C. antarctica, Hypozyma sp.…”

Candida antarctica Lipase B: An Ideal Biocatalyst for the Preparation of Nitrogenated Organic Compounds

“…The restricted active site of CAL-B can explain the excellent enantiopreference shown towards secondary alcohols and other nucleophiles such as primary amines. [11] Recently this biocatalyst has been object of several structural modifications using genetic engineering to improve its selectivity [12] and to create novel synthetic applications. [13] CAL-B has allowed the preparation of both non-chiral and chiral interesting amides and amines over the years.…”