Engineering Inorganic Materials with DNA Nanostructures

Heuer‐Jungemann, Amelie; Linko, Veikko

doi:10.1021/acscentsci.1c01272

Cited by 45 publications

(54 citation statements)

References 98 publications

(198 reference statements)

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“…[9,10] The low-Mg 2+ ion content and the presence of digestion enzymes can lead to the unraveling and structural breakdown of the DNA nanostructures, thus imposing a limit to their lifetime. [11] Therefore, measures have been taken to protect DNA nanostructures from their environment by encapsulation, [11][12][13][14][15][16] by transferring their structural information into other materials, [17][18][19][20][21] by chemical [22,23] or enzymatic ligation [24,25] of the staple strands, or by covalently cross-linking neighboring DNA domains. [26][27][28][29] However, environmental stability is not unambiguous for all DNA nanostructures.…”

Section: Introductionmentioning

confidence: 99%

Environment‐Dependent Stability and Mechanical Properties of DNA Origami Six‐Helix Bundles with Different Crossover Spacings

Yang

Piskunen

Suma

et al. 2022

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The internal design of DNA nanostructures defines how they behave in different environmental conditions, such as endonuclease‐rich or low‐Mg2+ solutions. Notably, the inter‐helical crossovers that form the core of such DNA objects have a major impact on their mechanical properties and stability. Importantly, crossover design can be used to optimize DNA nanostructures for target applications, especially when developing them for biomedical environments. To elucidate this, two otherwise identical DNA origami designs are presented that have a different number of staple crossovers between neighboring helices, spaced at 42‐ and 21‐ basepair (bp) intervals, respectively. The behavior of these structures is then compared in various buffer conditions, as well as when they are exposed to enzymatic digestion by DNase I. The results show that an increased number of crossovers significantly improves the nuclease resistance of the DNA origami by making it less accessible to digestion enzymes but simultaneously lowers its stability under Mg2+‐free conditions by reducing the malleability of the structures. Therefore, these results represent an important step toward rational, application‐specific DNA nanostructure design.

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Section: Introductionmentioning

confidence: 99%

Environment‐Dependent Stability and Mechanical Properties of DNA Origami Six‐Helix Bundles with Different Crossover Spacings

Yang

Piskunen

Suma

et al. 2022

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“… 2021 7 1969 1979 . 2 This work summarizes diverse approaches for creating nanoparticle assemblies using DNA nanostructures as templates. It further adds to the topics presented here by describing versatile metallization and mineralization schemes for DNA frameworks .…”

Section: Key Referencesmentioning

confidence: 99%

“…The addressability of DNA-based architectures enables engineering of various inorganic materials, from metallized or mineralized nanoshapes to crystals with defined configurations. 2 DNA-directed particle assembly is governed by simple rules, that is, valency . DNA-assisted NP crystals form via two design principles: (1) NP-templated DNA bonds, where customized and flexible DNA sequences act as guiding surface ligands for the core particles ( Figure 2 a) or (2) hybridization-based DNA bonds, where higher-order systems assemble from DNA motifs and frameworks hosting different cargo ( Figure 2 b).…”

Section: Dna-guided Assemblymentioning

confidence: 99%

From Precision Colloidal Hybrid Materials to Advanced Functional Assemblies

et al. 2022

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Conspectus The concept of colloids encompasses a wide range of isotropic and anisotropic particles with diverse sizes, shapes, and functions from synthetic nanoparticles, nanorods, and nanosheets to functional biological units. They are addressed in materials science for various functions, while they are ubiquitous in the biological world for multiple functions. A large variety of synthetic colloids have been researched due to their scientific and technological importance; still they characteristically suffer from finite size distributions, imperfect shapes and interactions, and not fully engineered functions. This contrasts with biological colloids that offer precision in their size, shape, and functionality. Materials science has searched for inspiration from the biological world to allow structural control by self-assembly and hierarchy and to identify novel routes for combinations of functions in bio-inspiration. Herein, we first discuss different approaches for highly defined structural control of technically relevant synthetic colloids based on guided assemblies of biological motifs. First, we describe how polydisperse nanoparticles can be assembled within hollow protein cages to allow well-defined assemblies and hierarchical packings. Another approach relies on DNA nanotechnology-based assemblies, where engineered DNA structures allow programmed assembly. Then we will discuss synthetic colloids that have either particularly narrow size dispersity or even atomically precise structures for new assemblies and potential functions. Such colloids can have well-defined packings for membranes allowing high modulus. They can be switchable using light-responsive moieties, and they can initiate packing of larger assemblies of different geometrical shapes. The emphasis is on atomically defined nanoclusters that allow well-defined assemblies by supramolecular interactions, such as directional hydrogen bonding. Finally, we will discuss stimulus-responsive colloids for new functions, even toward complex responsive functions inspired by life. Therein, stimulus-responsive materials inspired by biological learning could allow the next generation of such materials. Classical conditioning is among the simplest biological learning concepts, requiring two stimuli and triggerable memory. Therein we use thermoresponsive hydrogels with plasmonic gold nanoparticles and a spiropyran photoacid as a model. Heating is the unconditioned stimulus leading to melting of the thermoresponsive gel, whereas light (at a specified wavelength) originally leads to reduced pH without plasmonic or structural changes because of steric gel stabilization. Under heat-induced gel melting, light results in pH-decrease and chain-like aggregation of the gold nanoparticles, allowing a new plasmonic response. Thus, simultaneous heating and light irradiation allow conditioning for a newly derived stimulus, where the logic diagram is analogous to Pavlovian conditioning. The shown assemblies demonstrate the different functionalitie...

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“…biomedicine, 10,11 bioengineering, 12 nanoelectronics 13 and robotics, 14,15 but which may be equally used for high-throughput spatial arrangement of various components for nano photonic and materials science applications. 16,17 Arguably, the most common technique to form DNA-based templates is the versatile and robust DNA origami method [18][19][20][21] that enables outstanding addressability and precision. Although DNA itself is not electrically conductive nor optically very intriguing, there is a great deal of studies that have manifested the feasibility of DNA origami as high-resolution "pegboards" for directing the programmable assembly of nanocomponents e.g.…”

Section: Introductionmentioning

confidence: 99%