Engineering increases in sulfur amino acid contents in flax by overexpressing the yeast Met25 gene

Czuj, Tadeusz; Żuk, Magdalena; Starzycki, M.; Amir, Rachel; Szopa, Jan

doi:10.1016/j.plantsci.2009.08.008

Cited by 13 publications

(8 citation statements)

References 36 publications

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“…Furthermore, antioxidant activity was strongly correlated with improved resistance to the Fusarium infection. The antioxidant potential was also increased in flax by the overexpression of yeast Met25 gene coding for O-acetylhomoserine-Oacetylserine (OAH-OAS) sulfhydrylase which positively influenced flax resistance to the pathogen attack (Czuj et al, 2009). …”

Section: Application Of Genetic Engineeringmentioning

confidence: 99%

Biotechnology of fibrous flax in Europe and China

Kulma

Żuk

Long

et al. 2015

Industrial Crops and Products

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Section: Application Of Genetic Engineeringmentioning

confidence: 99%

Biotechnology of fibrous flax in Europe and China

Kulma

Żuk

Long

et al. 2015

Industrial Crops and Products

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“…Development of genetic engineering enabled generation of genetically modified plants characterized by increased resistance to pathogen infections. Flax that was more resistant to F. oxysporum and F. culmorum infection was generated by overexpression of genes involved in pathogenesis (PR genes) [ 40 ] and genes of secondary metabolites [ 41 – 43 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the significance of cell wall polymers in flax infected with a pathogenic strain of Fusarium oxysporum

et al. 2016

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BackgroundFusarium oxysporum infection leads to Fusarium-derived wilt, which is responsible for the greatest losses in flax (Linum usitatissimum) crop yield. Plants infected by Fusarium oxysporum show severe symptoms of dehydration due to the growth of the fungus in vascular tissues. As the disease develops, vascular browning and leaf yellowing can be observed. In the case of more virulent strains, plants die. The pathogen’s attack starts with secretion of enzymes degrading the host cell wall. The main aim of the study was to evaluate the role of the cell wall polymers in the flax plant response to the infection in order to better understand the process of resistance and develop new ways to protect plants against infection. For this purpose, the expression of genes involved in cell wall polymer metabolism and corresponding polymer levels were investigated in flax seedlings after incubation with Fusarium oxysporum.ResultsThis analysis was facilitated by selecting two groups of genes responding differently to the infection. The first group comprised genes strongly affected by the infection and activated later (phenylalanine ammonia lyase and glucosyltransferase). The second group comprised genes which are slightly affected (up to five times) and their expression vary as the infection progresses. Fusarium oxysporum infection did not affect the contents of cell wall polymers, but changed their structure.ConclusionThe results suggest that the role of the cell wall polymers in the plant response to Fusarium oxysporum infection is manifested through changes in expression of their genes and rearrangement of the cell wall polymers. Our studies provided new information about the role of cellulose and hemicelluloses in the infection process, the change of their structure and the expression of genes participating in their metabolism during the pathogen infection. We also confirmed the role of pectin and lignin in this process, indicating the major changes at the mRNA level of lignin metabolism genes and the loosening of the pectin structure.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0762-z) contains supplementary material, which is available to authorized users.

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“…High levels of cysteine can also affect the level of methionine (Czuj et al , ; Nguyen et al , ). Therefore, the levels of methionine, as well as the levels of other amino acids, were measured using gas chromatography‐mass spectrometry (GCMS).…”

Section: Resultsmentioning

confidence: 99%

Evidence of a significant role of glutathione reductase in the sulfur assimilation pathway

et al. 2020

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With the objective of studying the role of glutathione reductase (GR) in the accumulation of cysteine and methionine, we generated transgenic tobacco and Arabidopsis lines overexpressing the cytosolic AtGR1 and the plastidic AtGR2 genes. The transgenic plants had higher contents of cysteine and glutathione. To understand why cysteine levels increased in these plants, we also used gr1 and gr2 mutants. The results showed that the transgenic plants have higher levels of sulfite, cysteine, glutathione and methionine, which are downstream to adenosine 5 0 phosphosulfate reductase (APR) activity. However, the mutants had lower levels of these metabolites, while the sulfate content increased. A feeding experiment using 34 SO 4 2also showed that the levels of APR downstream metabolites increased in the transgenic lines and decreased in gr1 compared with their controls. These findings, and the results obtained from the expression levels of several genes related to the sulfur pathway, suggest that GR plays an essential role in the sulfur assimilation pathway by supporting the activity of APR, the key enzyme in this pathway. GR recycles the oxidized form of glutathione (GSSG) back to reduce glutathione (GSH), which serves as an electron donor for APR activity. The phenotypes of the transgenic plants and the mutants are not significantly altered under non-stress and oxidative stress conditions. However, when germinating on sulfur-deficient medium, the transgenic plants grew better, while the mutants were more sensitive than the control plants. The results give substantial evidence of the yet unreported function of GR in the sulfur assimilation pathway.

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Engineering increases in sulfur amino acid contents in flax by overexpressing the yeast Met25 gene

Cited by 13 publications

References 36 publications

Biotechnology of fibrous flax in Europe and China

Biotechnology of fibrous flax in Europe and China

Evaluation of the significance of cell wall polymers in flax infected with a pathogenic strain of Fusarium oxysporum

Evidence of a significant role of glutathione reductase in the sulfur assimilation pathway

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