Engineering immunomodulatory and osteoinductive implant surfaces via mussel adhesion-mediated ion coordination and molecular clicking

Abstract

Immune response and new tissue formation are important aspects of tissue repair. However, only a single aspect is generally considered in previous biomedical interventions, and the synergistic effect is unclear. Here, a dual-effect coating with immobilized immunomodulatory metal ions (e.g., Zn2+) and osteoinductive growth factors (e.g., BMP-2 peptide) is designed via mussel adhesion-mediated ion coordination and molecular clicking strategy. Compared to the bare TiO2 group, Zn2+ can increase M2 macrophage recruitment by up to 92.5% in vivo and upregulate the expression of M2 cytokine IL-10 by 84.5%; while the dual-effect of Zn2+ and BMP-2 peptide can increase M2 macrophages recruitment by up to 124.7% in vivo and upregulate the expression of M2 cytokine IL-10 by 171%. These benefits eventually significantly enhance bone-implant mechanical fixation (203.3 N) and new bone ingrowth (82.1%) compared to the bare TiO2 (98.6 N and 45.1%, respectively). Taken together, the dual-effect coating can be utilized to synergistically modulate the osteoimmune microenvironment at the bone-implant interface, enhancing bone regeneration for successful implantation.

Electrospun nanofibers for bone regeneration: from biomimetic composition, structure to function

“…228 As an illustration, Deng et al recently designed a dual-effect surface coating with immobilized immunomodulatory metal ions and osteoinductive growth factors via mussel adhesion-mediated ion coordination and molecular clicking strategy. 229 Benefiting from the synergistic effect of Zn 2+ and BMP-2, the surface-coated Ti implants demonstrated a remarkably more M2 macrophage recruitment and higher expression of M2 cytokine IL-10 in vivo , thus causing enhanced bone formation by up to 80% at 8 weeks, which was obviously higher than either stimulator alone. 229…”

“…229 Benefiting from the synergistic effect of Zn 2+ and BMP-2, the surface-coated Ti implants demonstrated a remarkably more M2 macrophage recruitment and higher expression of M2 cytokine IL-10 in vivo , thus causing enhanced bone formation by up to 80% at 8 weeks, which was obviously higher than either stimulator alone. 229…”

A Drug Delivery System Constructed by a Fusion Peptide Capturing Exosomes Targets to Titanium Implants Accurately Resulting the Enhancement of Osseointegration Peri-implant

“…[43] While a N1s speak at 395.0eV and an O1s speak at 530.0eV in XPS were derived from the amide groups of the Ti discs. [44] It suggested the PEP2 and PEP3 adsorbed on the Ti surface with a high density after washed by PBS, verifyied the PEP2 and PEP3 contained the functional of the motif TBP to speci cally adsorb on the titanium surface. Moreover, the result of IVIS indicating PEP2 and PEP3 could orientate to titanium implant in vivo for longer.…”

Biomimetic Implant Surfaces and Their Role in Biological Integration—A Concise Review

“…Besides that, while a large number of studies emphasize the role of surface topography in biological response, very few studies have evaluated the wettability, surface energy and chemical composition of dental implants [ 88 , 89 , 90 ]. Since different techniques to characterize surface parameters were used among studies, it is difficult to establish direct comparisons.…”