Engineering genome-reduced Bacillus subtilis for acetoin production from xylose

Ping, Yan; Wu, Yuanqing; Li, Yang; Wang, Zhiwen; Chen, Tao

doi:10.1007/s10529-017-2481-4

Cited by 19 publications

(11 citation statements)

References 15 publications

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“…subtilis and B. licheniformis have successfully been engineered for acetoin production, while E. cloacae has been shown to be a natural overproducer of acetoin. 6,13,40,41 The enzyme encoded by operon, acetolactate synthase and acetolactate decarboxylase, convert pyruvate into acetoin through acetolactate. Overexpression of the alsSD operon from B. subtilis in B. methanolicus MGA3 resulted in proof-of-concept for acetoin production from methanol.…”

Section: Introductionmentioning

confidence: 99%

Methanol-based acetoin production by genetically engineeredBacillus methanolicus

et al. 2020

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Section: Introductionmentioning

confidence: 99%

Methanol-based acetoin production by genetically engineeredBacillus methanolicus

et al. 2020

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“…In practice, the chassis cells are often modified as per the requirement, which entails embedding, replacing, or deleting genes or gene modules. Standard chassis cells include microbial cells, such as E. coli, B. subtilis, yeast , Pseudomonas, and Mycoplasma ( Yan et al, 2018 ; Jores et al, 2019 ; Zhuang and Qi, 2019 ; Liang et al, 2020 ; Liu et al, 2020 ). For example, the deletion of 581.9–814.4 non-essential gene sequence of B. subtilis by gene degeneration method mitigated the guanosine and thymidine production ( Li et al, 2016 ; Liu et al, 2018a ; Cui et al, 2018 ).…”

Section: Development Of B Subtilis As a Chassismentioning

confidence: 99%

Advances in Enhanced Menaquinone-7 Production From Bacillus subtilis

Liao

Ayansola

et al. 2021

Front. Bioeng. Biotechnol.

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The production of nutraceutical compounds through biosynthetic approaches has received considerable attention in recent years. For example, Menaquinone-7 (MK-7), a sub-type of Vitamin K2, biosynthesized from Bacillus subtilis (B. subtilis), proved to be more efficiently produced than the conventional chemical synthesis techniques. This is possible due to the development of B. subtilis as a chassis cell during the biosynthesis stages. Hence, it is imperative to provide insights on the B. subtilis membrane permeability modifications, biofilm reactors, and fermentation optimization as advanced techniques relevant to MK-7 production. Although the traditional gene-editing method of homologous recombination improves the biosynthetic pathway, CRISPR-Cas9 could potentially resolve the drawbacks of traditional genome editing techniques. For these reasons, future studies should explore the applications of CRISPRi (CRISPR interference) and CRISPRa (CRISPR activation) system gene-editing tools in the MK-7 anabolism pathway.

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“…Engineering and modifying microbial chassis could maximize its practical application ranges and obtain maximum theoretical yields of bio-based products of interests. Such as B. subtilis BSK814, a genome-reduced strain, was endowed with the ability to hyperproduce guanosine as well as acetoin by modifying different metabolic pathways [4,19].…”

Section: Genome Reduction Can Improve the Metabolic Capacitymentioning

confidence: 99%

“…In recent years, several metabolic engineering strategies have been proposed for enhancing biosurfactant production, mainly including promoter engineering [15][16][17], the reduction of by-product formation [11], the enhancement of the precursor supply [2], the improvement of biosurfactant transmembrane e ux [18], and the modi cation of global regulatory factors [19]. Among which, promoter engineering is highlighted as a powerful tool for enhancing the titer of biosurfactants.…”

Section: Introductionmentioning

confidence: 99%

Engineering of a genome-reduced strain Bacillus amyloliquefaciens for enhancing surfactin production

Zhang

Huo

Song

et al. 2020

Preprint

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Background: Genome reduction and metabolic engineering have emerged as intensive research hotspots for constructing the promising functional chassis and various microbial cell factories. Surfactin, a lipopeptide-type biosurfactant with broad spectrum antibiotic activity, has wide application prospects in anticancer therapy, biocontrol and bioremediation. Bacillus amyloliquefaciens LL3, previously isolated by our lab, contains an intact srfA operon in the genome for surfactin biosynthesis. Results: In this study, a genome-reduced strain GR167 lacking ~4.18% of the B. amyloliquefaciens LL3 genome was constructed by deleting some unnecessary genomic regions. Compared with the strain NK-1 (LL3 derivative, ΔuppΔpMC1), GR167 exhibited faster growth rate, higher transformation efficiency, increased intracellular reducing power level and higher heterologous protein expression capacity. Furthermore, the chassis strain GR167 was engineered for enhanced surfactin production. Firstly, the iturin and fengycin biosynthetic gene clusters were deleted from GR167 to generate GR167ID. Subsequently, two promoters PRsuc and PRtpxi from LL3 were obtained by RNA-seq and promoter strength characterization, and then they were individually substituted for the native srfA promoter in GR167ID to generate GR167IDS and GR167IDT. The best mutant GR167IDS showed a 678-fold improvement in the transcriptional level of the srfA operon relative to GR167ID, and it produced 311.35 mg/L surfactin, with a 10.4-fold increase relative to GR167. Conclusions: The genome-reduced strain GR167 was advantageous over the parental strain in several industrially relevant physiological traits assessed and it was highlighted as a chassis strain for further genetic modification. In future studies, further reduction of the LL3 genome can be expected to create high-performance chassis for synthetic biology applications.

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Engineering genome-reduced Bacillus subtilis for acetoin production from xylose

Abstract: As a chassis cell, genome-reduced B. subtilis showed significantly improved capacity for the production of the overflow product acetoin from xylose compared with wild-type strain.

Cited by 19 publications

References 15 publications

Methanol-based acetoin production by genetically engineeredBacillus methanolicus

Methanol-based acetoin production by genetically engineeredBacillus methanolicus

Advances in Enhanced Menaquinone-7 Production From Bacillus subtilis

Engineering of a genome-reduced strain Bacillus amyloliquefaciens for enhancing surfactin production

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