Engineering Escherichia coli with acrylate pathway genes for propionic acid synthesis and its impact on mixed-acid fermentation

Kandasamy, Vijayalakshmi; Vaidyanathan, Hema; Djurdjević, Ivana; Jayamani, Elamparithi; Ramachandran, K.B.; Buckel, Wolfgañg; Jayaraman, Guhan; Ramalingam, Subramanian

doi:10.1007/s00253-012-4274-y

Cited by 45 publications

(34 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The protein sequences of genes lcdA, lcdB and lcdC from the soil bacterium C. propionicum (accession numbers AEM62993-5), encoding the a-and b-subunits of the enzyme as well as the activator (Kandasamy et al, 2013), were blasted against non-redundant protein sequences at NCBI, and phylogenetic trees were generated from the blast results (data not shown). Genes belonging to a limited number of bacteria (C. propionicum, C. botulinum type C and D, C. novyi, Megasphaera spp., C. catus, Desulfosporosinus spp., Peptinophilus indolicus and Fusobacterium sp.)…”

Section: Acrylate Pathwaymentioning

confidence: 99%

“…This class of bacteria remains understudied in the human gut and it remains to be established which genera dominate in this ecosystem. The acrylate pathway for propionate formation has been characterised in detail in the soil bacterium Clostridium propionicum (Hetzel et al, 2003), and the corresponding genes have recently been described (Kandasamy et al, 2013). This pathway is also present in the rumen bacterium Megasphaera elsdenii within the Negativicutes (Hino and Kuroda, 1993), which produces butyrate during growth on glucose, but propionate during growth on lactate (Hino and Kuroda, 1993).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phylogenetic distribution of three pathways for propionate production within the human gut microbiota

et al. 2014

View full text Add to dashboard Cite

Propionate is produced in the human large intestine by microbial fermentation and may help maintain human health. We have examined the distribution of three different pathways used by bacteria for propionate formation using genomic and metagenomic analysis of the human gut microbiota and by designing degenerate primer sets for the detection of diagnostic genes for these pathways. Degenerate primers for the acrylate pathway (detecting the lcdA gene, encoding lactoylCoA dehydratase) together with metagenomic mining revealed that this pathway is restricted to only a few human colonic species within the Lachnospiraceae and Negativicutes. The operation of this pathway for lactate utilisation in Coprococcus catus (Lachnospiraceae) was confirmed using stable isotope labelling. The propanediol pathway that processes deoxy sugars such as fucose and rhamnose was more abundant within the Lachnospiraceae (based on the pduP gene, which encodes propionaldehyde dehydrogenase), occurring in relatives of Ruminococcus obeum and in Roseburia inulinivorans. The dominant source of propionate from hexose sugars, however, was concluded to be the succinate pathway, as indicated by the widespread distribution of the mmdA gene that encodes methylmalonyl-CoA decarboxylase in the Bacteroidetes and in many Negativicutes. In general, the capacity to produce propionate or butyrate from hexose sugars resided in different species, although two species of Lachnospiraceae (C. catus and R. inulinivorans) are now known to be able to switch from butyrate to propionate production on different substrates. A better understanding of the microbial ecology of short-chain fatty acid formation may allow modulation of propionate formation by the human gut microbiota.

show abstract

Section: Acrylate Pathwaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phylogenetic distribution of three pathways for propionate production within the human gut microbiota

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Nevertheless, several groups have reported propionate production from overexpression of the operon in complex media [82][83][84]. We suspect that the reported propionate production is a result a ghost-peak interfering with propionate quantification [45].…”

Section: Propionic Acid Biosynthesis By Non-native Producersmentioning

confidence: 99%

“…In the presence of glucose, net yields of 3 ATP/glucose and about 0.4 g propionate/g glucose (1 mol propionate/mol glucose) could be achieved, while higher yields can be achieved through the use of the pentose phosphate pathway with a concomitant energetic penalty (Table 5, Table 6). While this pathway has been successfully engineered into E. coli [45], initial yields were 2 orders of magnitude lower than the 0.4 g/g that would be expected. 1 To test whether synergistic interactions could occur between pathways to improve energy production, all glucose catabolising pathways leading to propionate production were allowed to carry flux, although performance did not improve over the Wood-Werkman cycle.…”

Section: 2-propanediol Associated Pathwaysmentioning

confidence: 99%

Microbial Propionic Acid Production

et al. 2017

View full text Add to dashboard Cite

Propionic acid (propionate) is a commercially valuable carboxylic acid produced through microbial fermentation. Propionic acid is mainly used in the food industry but has recently found applications in the cosmetic, plastics and pharmaceutical industries. Propionate can be produced via various metabolic pathways, which can be classified into three major groups: fermentative pathways, biosynthetic pathways, and amino acid catabolic pathways. The current review provides an in-depth description of the major metabolic routes for propionate production from an energy optimization perspective. Biological propionate production is limited by high downstream purification costs which can be addressed if the target yield, productivity and titre can be achieved. Genome shuffling combined with high throughput omics and metabolic engineering is providing new opportunities, and biological propionate production is likely to enter the market in the not so distant future. In order to realise the full potential of metabolic engineering and heterologous expression, however, a greater understanding of metabolic capabilities of the native producers, the fittest producers, is required.

show abstract

“…This pathway provides a source of ATP and carbon compounds that can be diverted to central metabolism via known pathways. The propanediol pathway is also present in Ruminococcus obeum [7] and Bacteroides thetaiotaomicron [15][16][17][18][19][20]. …”

Section: Propanediol Pathwaymentioning

confidence: 99%