Engineering Elevated Vitamin C in Plants to Improve their Nutritional Content, Growth, and Tolerance to Abiotic Stress

Lisko, Katherine A.; Aboobucker, Siddique I.; Torres, Raquel; Lorence, Argelia

doi:10.1007/978-3-319-04045-5_6

Cited by 31 publications

(33 citation statements)

References 102 publications

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“…The possibilities of differential substrate uptake (Baig et al, 1970; Davey et al, 1999) and the stimulation of AsA synthesis by a general increase in carbohydrate pool (Loewus, 1963; Baig et al, 1970) have been ruled out. Iinterestingly, when Wheeler et al (2015) fed l -Gal or l -GulL to the rhodophyte algae Galdieria sulphuraria , the phenomenon of AsA increase observed was just as it has been observed in many plant species as mentioned above (Isherwood et al, 1954; Lisko et al, 2014). Rightfully so, Wheeler et al (2015) concluded that G. sulphuraria has a GulLO enzyme instead of a GLDH.…”

Section: Plants Have Gullos In Addition To Gldhssupporting

confidence: 67%

“…First, multiple precursor feeding studies have reported the use of l -GulL as a substrate since 1954 (Isherwood et al, 1954) including our observation in the detached leaves of Nicotiana benthamiana (wood tobacco; Lisko et al, 2014). The increase in AsA when fed with l -GulL is different than with l -GalL or l -Gal feeding.…”

Section: Plants Have Gullos In Addition To Gldhsmentioning

confidence: 89%

“…In the past, enzymes from the AsA biosynthetic and recycling pathways have been overexpressed to increase AsA in plants (reviewed in Lisko et al, 2014). Ascorbate biosynthetic pathways in plants converge to either l -GulL or l -GalL as the immediate precursor.…”

Section: Discussionmentioning

confidence: 99%

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Recent progress on the characterization of aldonolactone oxidoreductases

Aboobucker

Lorence

2016

Plant Physiology and Biochemistry

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l-Ascorbic acid (ascorbate, AsA, vitamin C) is essential for animal and plant health. Despite our dependence on fruits and vegetables to fulfill our requirement for this vitamin, the metabolic network leading to its formation in plants is just being fully elucidated. There is evidence supporting the operation of at least four biosynthetic pathways leading to AsA formation in plants. These routes use d-mannose/l-Galactose, l-gulose, d-galacturonate, and myo-inositol as the main precursors. This review focuses on aldonolactone oxidoreductases, a subgroup of the vanillyl alcohol oxidase (VAO; EC 1.1.3.38) superfamily, enzymes that catalyze the terminal step in AsA biosynthesis in bacteria, protozoa, animals, and plants. In this report, we review the properties of well characterized aldonolactone oxidoreductases to date. A shared feature in these proteins is the presence of a flavin cofactor as well as a thiol group. The flavin cofactor in many cases is bound to the N terminus of the enzymes or to a recently discovered HWXK motif in the C terminus. The binding between the flavin moiety and the protein can be either covalent or non-covalent. Substrate specificity and subcellular localization differ among the isozymes of each kingdom. All oxidases among these enzymes possess dehydrogenase activity, however, exclusive dehydrogenases are also found. We also discuss recent evidence indicating that plants have both l-gulono-1,4-lactone oxidases and l-Galactono-1,4-lactone dehydrogenases involved in AsA biosynthesis.

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Section: Plants Have Gullos In Addition To Gldhssupporting

confidence: 67%

Section: Plants Have Gullos In Addition To Gldhsmentioning

confidence: 89%

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Recent progress on the characterization of aldonolactone oxidoreductases

Aboobucker

Lorence

2016

Plant Physiology and Biochemistry

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“…It controls cell division and cell expansion, acts as a cofactor of many enzymes, modulates plant sense, and is involved in photosynthesis, hormone biosynthesis, and regeneration of antioxidants (Gallie, 2012; Lisko et al, 2014). Although AsA is found in different plant tissues, its levels are abundant in meristems, photosynthetic cells and different fruits (HongBo et al, 2005; Mazid et al, 2011).…”

Section: Ascorbic Acid and Abiotic Stress Conditionsmentioning

confidence: 99%

Ascorbic Acid-A Potential Oxidant Scavenger and Its Role in Plant Development and Abiotic Stress Tolerance

2017

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Over-production of reactive oxygen species (ROS) in plants under stress conditions is a common phenomenon. Plants tend to counter this problem through their ability to synthesize ROS neutralizing substances including non-enzymatic and enzymatic antioxidants. In this context, ascorbic acid (AsA) is one of the universal non-enzymatic antioxidants having substantial potential of not only scavenging ROS, but also modulating a number of fundamental functions in plants both under stress and non-stress conditions. In the present review, the role of AsA, its biosynthesis, and cross-talk with different hormones have been discussed comprehensively. Furthermore, the possible involvement of AsA-hormone crosstalk in the regulation of several key physiological and biochemical processes like seed germination, photosynthesis, floral induction, fruit expansion, ROS regulation and senescence has also been described. A simplified and schematic AsA biosynthetic pathway has been drawn, which reflects key intermediates involved therein. This could pave the way for future research to elucidate the modulation of plant AsA biosynthesis and subsequent responses to environmental stresses. Apart from discussing the role of different ascorbate peroxidase isoforms, the comparative role of two key enzymes, ascorbate peroxidase (APX) and ascorbate oxidase (AO) involved in AsA metabolism in plant cell apoplast is also discussed particularly focusing on oxidative stress perception and amplification. Limited progress has been made so far in terms of developing transgenics which could over-produce AsA. The prospects of generation of transgenics overexpressing AsA related genes and exogenous application of AsA have been discussed at length in the review.

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“…The damage caused to plants as a consequence of high O 3 concentrations could be mitigated or prevented by application of antioxidants, such as ascorbic acid, glutathione, and the antiozonant ethylenediurea (EDU) (Didyk and Blum, 2010;Feng et al, 2010;Lisko et al, 2014;Manning, 2000;Manning et al, 2011;Paoletti et al, 2009;Saitanis et al, 2015). These antioxidants and EDU have been extensively used in USA and many European countries to protect crop yield loss from ambient ozone.…”

Section: Applying Chemical Protective Agents At Key Phenological Stagesmentioning

confidence: 99%