Engineering electrochemical CO<sub>2</sub> reduction to formate under bioprocess‐compatible conditions to bioreactor scale

Hegner, Richard; Neubert, Katharina; Rosa, Luís F. M.

doi:10.1002/celc.201900526

Cited by 10 publications

(19 citation statements)

References 31 publications

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“…[12,19] When combining electroreduction to form intermediates with bioproduction, different approaches are feasible (Scheme 1). One promising option are secondary microbial electrochemical technologies (MET), locating the initial electrochemical reduction in-situ to the microbial biosynthesis, [20,21] as it has already been shown for formate [22,23] and H 2 . [24] However, scale-up issues and non-ideal conditions of the electrochemical set up pose major challenges for establishing in-situ production with direct bioconversion in industrial scale.…”

Section: Introductionmentioning

confidence: 99%

From CO₂ to Bioplastic – Coupling the Electrochemical CO₂ Reduction with a Microbial Product Generation by Drop‐in Electrolysis

et al. 2020

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CO2 has been electrochemically reduced to the intermediate formate, which was subsequently used as sole substrate for the production of the polymer polyhydroxybutyrate (PHB) by the microorganism Cupriavidus necator. Faradaic efficiencies (FE) up to 54 % have been reached with Sn‐based gas‐diffusion electrodes in physiological electrolyte. The formate containing electrolyte can be used directly as drop‐in solution in the following biological polymer production by resting cells. 56 mg PHB L−1 and a ratio of 34 % PHB per cell dry weight were achieved. The calculated overall FE for the process was as high as 4 %. The direct use of the electrolyte as drop‐in media in the bioconversion enables simplified processes with a minimum of intermediate purification effort. Thus, an optimal coupling between electrochemical and biotechnological processes can be realized.

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Section: Introductionmentioning

confidence: 99%

From CO₂ to Bioplastic – Coupling the Electrochemical CO₂ Reduction with a Microbial Product Generation by Drop‐in Electrolysis

et al. 2020

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“…Here, during the last years not only key process indicators like CE and rate of the CO 2 to formic acid conversion were significantly improved to values that can allow an integration of the abiotic electrosynthesis with the microbial conversion, [ 37 ] but also its scaling to 1 L electrobioreactors was demonstrated successfully. [ 38 ] With the concept of providing the microbial substrate via electrochemistry Krieg et al. achieved the production of terpenes from CO 2 using electrochemically generated H 2 as microbial energy source.…”

Section: Mes Using Secondary Met and Hybrid Systemsmentioning

confidence: 99%

Microbial Electrosynthesis—An Inventory on Technology Readiness Level and Performance of Different Process Variants

Fruehauf

Enzmann

Harnisch

et al. 2020

Biotechnology Journal

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The objective of microbial electrosynthesis (MES) is to combine the advantages of electrochemistry and biotechnology in order to produce chemicals and fuels. This combination enables resource-efficient processes by using renewable raw materials and regenerative energies. In the last decade, different MES processes have been described, for example, MES based on biofilms or mediators, electro-fermentation, and secondary MES. This review compares the MES technologies with regard to the reached process performances in terms of key process indicators (i.e., coulombic efficiency (CE), product titre, productivity) and technology readiness level (TRL). Often the underlying mechanism of electron transfer in biofilm-based processes has not been elucidated and can therefore not be optimized. Similarly, technical aspects of electro-fermentation processes and processes with soluble mediators are under investigation and techno-economic assessments are missing. In contrast, the electrochemical production of microbial substrates in secondary MES or hybrid systems show high key process indicators and TRLs up to 7. In summary, the different types of MES processes offer options for today's industrial use, as well as an exciting and future-oriented technology that can be applied in a medium-term perspective.

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“…demonstrated (Hegner et al, 2019). Here we focused on the sterilizability of the inlay of the upgrade kit housing the ion exchange membrane.…”

Section: Sterilization Of the Inlay With Membranementioning

confidence: 99%

Integrating Electrochemistry Into Bioreactors: Effect of the Upgrade Kit on Mass Transfer, Mixing Time and Sterilizability

et al. 2019

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Microbial electrosynthesis (MES) is an exciting and dynamic research area at the nexus of microbiology and electrochemistry. To pave the way of MES to application, reactor infrastructure is needed that meets the requirements of both biological, and electrochemical engineering. Recently we presented an upgrade kit facilitating turning commercial bioreactors based on batch stirred tank reactors (BSTR) into electrobioreactors that can be scaled and benchmarked. The upgrade kit comprises electrodes and an inlay with membrane, separating the BSTR chamber into anode and cathode compartments. The obtained electrobioreactors enable seizing the existing infrastructure for monitoring and controlling the bioprocess while being complemented by electrochemical control and measurement. Here the effect of the upgrade kit on mass transfer was investigated in a 1 L electrobioreactor using experimental approaches and modeling of fluid dynamics. It is shown that the fluid dynamics in the BSTR are changed dramatically, impacting the integral parameters volumetric mass transfer coefficient, k L a, and mixing time, θ. The influences of the inlay chamber and electrodes as well as stirrer position and geometry are described. Additionally, the sterilizability of the inlay, housing the polymer-based membrane of the electrobioreactor, which is needed for operating two-chamber MES based on microbial pure cultures, are demonstrated.

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Engineering electrochemical CO₂ reduction to formate under bioprocess‐compatible conditions to bioreactor scale

Cited by 10 publications

References 31 publications

From CO₂ to Bioplastic – Coupling the Electrochemical CO₂ Reduction with a Microbial Product Generation by Drop‐in Electrolysis

From CO₂ to Bioplastic – Coupling the Electrochemical CO₂ Reduction with a Microbial Product Generation by Drop‐in Electrolysis

Microbial Electrosynthesis—An Inventory on Technology Readiness Level and Performance of Different Process Variants

Integrating Electrochemistry Into Bioreactors: Effect of the Upgrade Kit on Mass Transfer, Mixing Time and Sterilizability

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Engineering electrochemical CO2 reduction to formate under bioprocess‐compatible conditions to bioreactor scale

Cited by 10 publications

References 31 publications

From CO2 to Bioplastic – Coupling the Electrochemical CO2 Reduction with a Microbial Product Generation by Drop‐in Electrolysis

From CO2 to Bioplastic – Coupling the Electrochemical CO2 Reduction with a Microbial Product Generation by Drop‐in Electrolysis

Microbial Electrosynthesis—An Inventory on Technology Readiness Level and Performance of Different Process Variants

Integrating Electrochemistry Into Bioreactors: Effect of the Upgrade Kit on Mass Transfer, Mixing Time and Sterilizability

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Engineering electrochemical CO₂ reduction to formate under bioprocess‐compatible conditions to bioreactor scale

From CO₂ to Bioplastic – Coupling the Electrochemical CO₂ Reduction with a Microbial Product Generation by Drop‐in Electrolysis

From CO₂ to Bioplastic – Coupling the Electrochemical CO₂ Reduction with a Microbial Product Generation by Drop‐in Electrolysis