Engineering drought and salinity tolerance traits in crops through CRISPR-mediated genome editing: Targets, tools, challenges, and perspectives

Shelake, Rahul Mahadev; Kadam, Ulhas Sopanrao; Kumar, Ritesh; Pramanik, Dibyajyoti; Singh, Anil Kumar; Kim, Jae‐Yean

doi:10.1016/j.xplc.2022.100417

Cited by 64 publications

(46 citation statements)

References 221 publications

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“…This can be observed in the example of mitochondrial CAs [ 49 ], and it seems that this is also the case in chloroplasts [ 5 ]. The potential outcome of using this approach to switch off genes encoding different types of CAs in A. thaliana is that the use of CRISPR/Cas9 editing in the multiplex variant [ 20 ] can lead to the knockout of two or more genes in the genome of one plant. Thus, researchers may have an excellent opportunity to produce mutant plant lines with multiple knockouts of chloroplast CAs genes, since these enzymes are convenient models for elucidating their functions and contributions to plant responses to different stresses.…”

Section: Discussionmentioning

confidence: 99%

“…The methods of molecular biology make it possible to purposefully modify certain genes of interest in order to increase our knowledge and improve some economically valuable traits of important agricultural crops. The development and improvement of these methods allow, firstly, for the identification of the mechanisms underlying the responses controlled by a large number of plant genes [ 20 ]. Mutations are a convenient tool for the identification of the function of the proteins encoded by the genes, in which normal functioning has been disrupted by this insertion [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

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The Role of Carbonic Anhydrase αCA4 in Photosynthetic Reactions in Arabidopsis thaliana Studied, Using the Cas9 and T-DNA Induced Mutations in Its Gene

Rudenko

Permyakova

Ignatova

et al. 2022

Plants

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An homozygous mutant line of Arabidopsis thaliana with a knocked out At4g20990 gene encoding thylakoid carbonic anhydrase αCA4 was created using a CRISPR/Cas9 genome editing system. The effects of the mutation were compared with those in two mutant lines obtained by the T-DNA insertion method. In αCA4 knockouts of all three lines, non-photochemical quenching of chlorophyll a fluorescence was lower than in the wild type (WT) plants due to a decrease in its energy-dependent component. The αCA4 knockout also affected the level of expression of the genes encoding all proteins of the PSII light harvesting antennae, the genes encoding cytoplasmic and thylakoid CAs and the genes induced by plant immune signals. The production level of starch synthesis during the light period, as well as the level of its utilization during the darkness, were significantly higher in these mutants than in WT plants. These data confirm that the previously observed differences between insertional mutants and WT plants were not the result of the negative effects of T-DNA insertion transgenesis but the results of αCA4 gene knockout. Overall, the data indicate the involvement of αCA4 in the photosynthetic reactions in the thylakoid membrane, in particular in processes associated with the protection of higher plants’ photosynthetic apparatus from photoinhibition.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Role of Carbonic Anhydrase αCA4 in Photosynthetic Reactions in Arabidopsis thaliana Studied, Using the Cas9 and T-DNA Induced Mutations in Its Gene

Rudenko

Permyakova

Ignatova

et al. 2022

Plants

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“…The excessive uptake of ions can activate diverse protection mechanisms, and different plant species have varying defense strategies. Plants sense salinity through the cells of the roots that are in direct contact with salt ions [ 77 ].…”

Section: Mechanism Of Signal Transduction and The Development Of Sali...mentioning

confidence: 99%

Molecular Responses of Vegetable, Ornamental Crops, and Model Plants to Salinity Stress

Toscano

Romano

Ferrante

2023

IJMS

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Vegetable and ornamental plants represent a very wide group of heterogeneous plants, both herbaceous and woody, generally without relevant salinity-tolerant mechanisms. The cultivation conditions—almost all are irrigated crops—and characteristics of the products, which must not present visual damage linked to salt stress, determine the necessity for a deep investigation of the response of these crops to salinity stress. Tolerance mechanisms are linked to the capacity of a plant to compartmentalize ions, produce compatible solutes, synthesize specific proteins and metabolites, and induce transcriptional factors. The present review critically evaluates advantages and disadvantages to study the molecular control of salt tolerance mechanisms in vegetable and ornamental plants, with the aim of distinguishing tools for the rapid and effective screening of salt tolerance levels in different plants. This information can not only help in suitable germplasm selection, which is very useful in consideration of the high biodiversity expressed by vegetable and ornamental plants, but also drive the further breeding activities.

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“…After the discovery of CRISPR/Cas, several tools based on this system were developed, allowing editing of target genomic loci and beyond [ 20 ]. Such tools include DNA base editors (BEs), epigenetic modifiers, prime editors (PEs), and transcription regulators (CRISPRa and CRISPRi) [ 21 ]. Several studies on CRISPR/Cas9 in plants have been conducted, both in monocotyledons and dicotyledons related to different abiotic stresses, such as osmotic stress in Arabidopsis thaliana [ 22 ], salinity in tomato [ 23 ], and water deficit in wheat [ 13 ], rice [ 24 ], chickpea [ 25 ], and corn [ 26 ], as well as temperature extremes in soybean and grapevine [ 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Gene Editing for Plant Resistance to Abiotic Factors: A Systematic Review

Nascimento

Rocha

Soares

et al. 2023

Plants

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Agricultural crops are exposed to various abiotic stresses, such as salinity, water deficits, temperature extremes, floods, radiation, and metal toxicity. To overcome these challenges, breeding programs seek to improve methods and techniques. Gene editing by Clustered Regularly Interspaced Short Palindromic Repeats—CRISPR/Cas—is a versatile tool for editing in all layers of the central dogma with focus on the development of cultivars of plants resistant or tolerant to multiple biotic or abiotic stresses. This systematic review (SR) brings new contributions to the study of the use of CRISPR/Cas in gene editing for tolerance to abiotic stress in plants. Articles deposited in different electronic databases, using a search string and predefined inclusion and exclusion criteria, were evaluated. This SR demonstrates that the CRISPR/Cas system has been applied to several plant species to promote tolerance to the main abiotic stresses. Among the most studied crops are rice and Arabidopsis thaliana, an important staple food for the population, and a model plant in genetics/biotechnology, respectively, and more recently tomato, whose number of studies has increased since 2021. Most studies were conducted in Asia, specifically in China. The Cas9 enzyme is used in most articles, and only Cas12a is used as an additional gene editing tool in plants. Ribonucleoproteins (RNPs) have emerged as a DNA-free strategy for genome editing without exogenous DNA. This SR also identifies several genes edited by CRISPR/Cas, and it also shows that plant responses to stress factors are mediated by many complex-signaling pathways. In addition, the quality of the articles included in this SR was validated by a risk of bias analysis. The information gathered in this SR helps to understand the current state of CRISPR/Cas in the editing of genes and noncoding sequences, which plays a key role in the regulation of various biological processes and the tolerance to multiple abiotic stresses, with potential for use in plant genetic improvement programs.

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Engineering drought and salinity tolerance traits in crops through CRISPR-mediated genome editing: Targets, tools, challenges, and perspectives

Cited by 64 publications

References 221 publications

The Role of Carbonic Anhydrase αCA4 in Photosynthetic Reactions in Arabidopsis thaliana Studied, Using the Cas9 and T-DNA Induced Mutations in Its Gene

The Role of Carbonic Anhydrase αCA4 in Photosynthetic Reactions in Arabidopsis thaliana Studied, Using the Cas9 and T-DNA Induced Mutations in Its Gene

Molecular Responses of Vegetable, Ornamental Crops, and Model Plants to Salinity Stress

Gene Editing for Plant Resistance to Abiotic Factors: A Systematic Review

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