Engineering Delftia acidovorans DSM39 to produce polyhydroxyalkanoates from slaughterhouse waste

Romanelli, Maria; Povolo, Silvana; Favaro, Lorenzo; Fontana, Federico; Basaglia, Marina; Casella, Sergio

doi:10.1016/j.ijbiomac.2014.03.049

Cited by 35 publications

(25 citation statements)

References 28 publications

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“…As a result, mcl ‐PHA from lard as substrate was produced by the recombinant P. putida . Later on, Delftia acidovorans was engineered for the co‐expression of efficient lipase genes ( lipC and lipH from a lipolytic P. stutzeri isolate), with the aim of processing slaughterhouse byproducts into PHA with 4HB fractions . Indeed, D. acidovorans is known to produce PHAs with high molar fractions of 4HB but, unfortunately, it is unable to grow on fatty substrates.…”

Section: Bacterial Strains Producing Phas Can Be Modified To Use Diffmentioning

confidence: 99%

“…Indeed, D. acidovorans is known to produce PHAs with high molar fractions of 4HB but, unfortunately, it is unable to grow on fatty substrates. The recombinant strain obtained displayed significant extracellular lipase activity on corn oil and the PHAs produced achieved 26% of CDM, with about 7 mol% of 4HB …”

Section: Bacterial Strains Producing Phas Can Be Modified To Use Diffmentioning

confidence: 99%

“…Later on, Delftia acidovorans was engineered for the co-expression of efficient lipase genes (lipC and lipH from a lipolytic P. stutzeri isolate), with the aim of processing slaughterhouse byproducts into PHA with 4HB fractions. 68 Indeed, D. acidovorans is known to produce PHAs with high molar fractions of 4HB but, unfortunately, it is unable to grow on fatty substrates. The recombinant strain obtained displayed significant extracellular lipase activity on corn oil and the PHAs produced achieved 26% of CDM, with about 7 mol% of 4HB.…”

Section: Waste Lipids As Carbon Sourcementioning

confidence: 99%

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Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review

Favaro

Basaglia

Casella

2018

Biofuels Bioprod Bioref

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132

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Polyhydroxyalkanoates (PHAs) are a family of biodegradable intracellular polyesters that a number of Eubacteria and Archaea can accumulate for energy and carbon storage. Most of the genetic modifications to the producing bacterial species have been accomplished to clarify basic biochemical, genetic, and metabolic aspects of PHA metabolism. However, due to its plastic‐like properties and complete biodegradability, this bio‐based polymer has attracted the attention of a variety of manufacturers. A number of genetic approaches have therefore been reported, aimed at improving the performance of the microorganisms with a potential for use in a production process. Indeed, genetic tools may find useful applications in all the phases of the PHA production chain, from the isolation and characterization of new microbial strains through all the production steps until they reach the downstream processes. The substrates generally used for PHA production are expensive, so the search for low‐cost feedstock is necessary. These materials, possibly deriving from agri‐food processes, are unfortunately not easily degraded or converted directly into PHAs. Thus, the development of engineered microbes is in progress to process waste streams and covert them to valuable polymers. This review will summarize the most relevant results obtained through genetic engineering tools for the production of PHAs from cheap carbon sources in view of possible industrial applications. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd.

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Section: Bacterial Strains Producing Phas Can Be Modified To Use Diffmentioning

confidence: 99%

Section: Bacterial Strains Producing Phas Can Be Modified To Use Diffmentioning

confidence: 99%

Section: Waste Lipids As Carbon Sourcementioning

confidence: 99%

See 1 more Smart Citation

Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review

Favaro

Basaglia

Casella

2018

Biofuels Bioprod Bioref

Self Cite

132

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“…The strain was found to be able to grow on a number of fatty substrates and, before approaching more complex fats as carbon source, a more accurate growth and PHA production test was assessed with corn oil, due to its emulsion easiness. With corn oil as substrate, the content of extracellular lipase units in the supernatant reached 0.47 U mL -1 after 72 h growth, as determined by the titrimetric assay previously described 40 . Following this result, other fatty substrates with different complexity were tested, such as glycerol, some methylesters derived from different fatty acids (myristic, palmitic and stearic methyl esters) and oleic acid, up to directly using animal fatty waste (bacon rind, udder, tallow).…”

Section: Production Of Poly(hydroxyalkanoates) By C Necator Dsm 545 mentioning

confidence: 99%

Poly(hydroxyalkanoate) Production by Cupriavidus necator from Fatty Waste Can Be Enhanced by phaZ1 Inactivation

Povolo

Basaglia

Fontana

et al. 2015

Chem.Biochem.Eng.Q.

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PHA production from waste oils or fats requires microorganisms that should be both excellent PHA producers and equipped with enzymatic activities allowing hydrolysation of triglycerides. Unfortunately, microbes with the combination of substrate-utilization and PHA production are not currently available, and the strategies to be adopted are the use of costly commercial enzymes, or genetic modification of microorganisms exhibiting high PHA product yields.In the present work, after a general investigation on the ability of Cupriavidus necator to grow on a number of fatty substrates, the possibility to enhance PHA production by limiting intracellular depolymerisation, was investigated. By knocking out the related phaZ1 gene, the construction of C. necator recombinant strains impaired in depolymerase (PhaZ1) activity was achieved. The polymer yield of the recombinant strain was finally compared to that of the parental C. necator DSM 545.

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“…The previously mentioned studies, though achieving promising results, demonstrate that the total exploitation of such substrates still needs to be addressed and that there is an opportunity to further increase the hydrolysis and fermentation yields from agricultural by-products containing different polysaccharides. Cheap and plentiful residual biomass has been investigated as renewable material to be converted into fuels, polymers, enzymes and bulk chemicals [20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Utilisation of wheat bran as a substrate for bioethanol production using recombinant cellulases and amylolytic yeast

et al. 2015

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Wheat bran, generated from the milling of wheat, represents a promising feedstock for the production of bioethanol. This substrate consists of three main components: starch, hemicellulose and cellulose. The optimal conditions for wheat bran hydrolysis have been determined using a recombinant cellulase cocktail (RCC), which contains two cellobiohydrolases, an endoglucanase and a beta-glucosidase. The 10% (w/v, expressed in terms of dry matter) substrate loading yielded the most glucose, while the 2% loading gave the best hydrolysis efficiency (degree of saccharification) using unmilled wheat bran. The ethanol production of two industrial amylolytic Saccharomyces cerevisiae strains, MEL2[TLG1-SFA1] and M2n [TLG1-SFA1], were compared in a simultaneous saccharification and fermentation (SSF) for 10% wheat bran loading with or without the supplementation of optimised RCC. The recombinant yeasts. cerevisiae MEL2[TLG1-SFA1] and M2n[TLG1-SFA1] completely hydrolysed wheat bran's starch producing similar amounts of ethanol (5.3 +/- 0.14 g/L and 5.0 +/- 0.09 g/L, respectively). Supplementing SSF with RCC resulted in additional ethanol production of about 2.0 g/L. Scanning electron microscopy confirmed the effectiveness of both RCC and engineered amylolytic strains in terms of cellulose and starch depolymerisatio

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Engineering Delftia acidovorans DSM39 to produce polyhydroxyalkanoates from slaughterhouse waste

Cited by 35 publications

References 28 publications

Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review

Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review

Poly(hydroxyalkanoate) Production by Cupriavidus necator from Fatty Waste Can Be Enhanced by phaZ1 Inactivation

Utilisation of wheat bran as a substrate for bioethanol production using recombinant cellulases and amylolytic yeast

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