Engineering cyanobacteria as cell factories for direct trehalose production from CO2

Qiao, Yue; Lü, Xuefeng

doi:10.1016/j.ymben.2020.08.014

Cited by 33 publications

(24 citation statements)

References 39 publications

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“…Furthermore, salt acclimation is becoming more important for applied research with cyanobacteria regarding the direct use of compatible solutes as well as mass cultivation in sea water to make the process more sustainable [ 9 , 68 ]. For example, the cyanobacterial production of mannitol [ 106 ] and trehalose [ 78 ] has been promoted by cultivation at enhanced salinities. Moreover, a more salt-tolerant version of the fast-growing Synechococcus elongatus strain UTEX2973 has been engineered by the expression of GG synthesis genes, which can be used for biotechnological purposes in full marine waters [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

Integrative analysis of the salt stress response in cyanobacteria

et al. 2021

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Microorganisms evolved specific acclimation strategies to thrive in environments of high or fluctuating salinities. Here, salt acclimation in the model cyanobacterium Synechocystis sp. PCC 6803 was analyzed by integrating transcriptomic, proteomic and metabolomic data. A dynamic reorganization of the transcriptome occurred during the first hours after salt shock, e.g. involving the upregulation of genes to activate compatible solute biochemistry balancing osmotic pressure. The massive accumulation of glucosylglycerol then had a measurable impact on the overall carbon and nitrogen metabolism. In addition, we observed the coordinated induction of putative regulatory RNAs and of several proteins known for their involvement in other stress responses. Overall, salt-induced changes in the proteome and transcriptome showed good correlations, especially among the stably up-regulated proteins and their transcripts. We define an extended salt stimulon comprising proteins directly or indirectly related to compatible solute metabolism, ion and water movements, and a distinct set of regulatory RNAs involved in post-transcriptional regulation. Our comprehensive data set provides the basis for engineering cyanobacterial salt tolerance and to further understand its regulation.

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Section: Discussionmentioning

confidence: 99%

Integrative analysis of the salt stress response in cyanobacteria

et al. 2021

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“…Furthermore, salt acclimation is becoming more important for applied research with cyanobacteria regarding the direct use of compatible solutes as well as mass cultivation in sea water to make the process more sustainable (Pade and Hagemann, 2014; Cui et al, 2020). For example, the cyanobacterial production of mannitol (Wu et al, 2020) and trehalose (Qiao et al, 2020) has been promoted by cultivation at enhanced salinities. Moreover, a more salt-tolerant version of the fast-growing Synechococcus elongatus strain UTEX2973 has been engineered by the expression of GG synthesis genes, which can be used for biotechnological purposes in full marine waters (Cui et al, 2021) However, saline conditions might also negatively affect the production titer.…”

Section: Discussionmentioning

confidence: 99%

Section: Future Developments -Regulation and Applicationmentioning

confidence: 99%

Integrative analysis of the salt stress response in cyanobacteria

Klaehn

Mikkat

Riediger

et al. 2021

Preprint

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Microorganisms evolved specific acclimation strategies to thrive in environments of high or fluctuating salinities. Here, salt acclimation in the model cyanobacterium Synechocystis sp. PCC 6803 was analyzed by integrating transcriptomic, proteomic and metabolomic data. A dynamic reorganization of the transcriptome and proteome occurred during the first hours after salt shock, e.g. involving the upregulation of genes to activate compatible solute biochemistry balancing osmotic pressure. The massive accumulation of glucosylglycerol then had a measurable impact on the overall carbon and nitrogen metabolism. In addition, we observed the coordinated induction of putative regulatory RNAs and of several proteins known for their involvement in other stress responses. Overall, salt-induced changes in the proteome and transcriptome showed good correlations, especially among the stably up-regulated proteins and their transcripts. We define an extended salt stimulon comprising proteins directly or indirectly related to compatible solute metabolism, ion and water movements, and a distinct set of regulatory RNAs involved in post-transcriptional regulation. Our comprehensive data set provides the basis for engineering cyanobacterial salt tolerance and to further understand its regulation.

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“…Synechococcus PCC 11801 and PCC 11901 (hereafter, Synechococcus 11801 and Synechococcus 11901) are also becoming popular for biotechnological purposes (4,5). To date, cyanobacteria have been exploited to convert CO 2 into a wide range of valuable products, for instance: biofuels (6, 7); commercial terpenoids (8, 9); polymeric compounds useful for bioplastic materials (10); bioactive compounds and vitamins (11); sugars (12); and pigments with potent antioxidant activity (13). To enable metabolic engineering in the different cyanobacterial strains, advanced genetic tools are emerging, from conventional methodologies to innovations in gene expression, genome editing and regulation systems (14)(15)(16)(17)(18)(19).…”

Section: Introductionmentioning

confidence: 99%

SEVA-Cpf1, a CRISPR-Cas12a vector for Genome Editing in Cyanobacteria

Baldanta

Guevara

Lloréns

2022

Preprint

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Background: Cyanobacteria are photosynthetic autotrophs that have tremendous potential for fundamental research and industrial applications due to their high metabolic plasticity and ability to grow using CO2 and sunlight. CRISPR technology using Cas9 and Cpf1 has been applied to different cyanobacteria for genome manipulations and metabolic engineering. Despite significant advances with genome editing in several cyanobacteria strains, the lack of proper genetic toolboxes is still a limiting factor compared to other model laboratory species. Among the limitations, it is essential to have versatile plasmids that could ease the benchwork when using CRISPR technology. Results: In the present study, several CRISPR-Cpf1 vectors were developed for genetic manipulations in cyanobacteria using SEVA plasmids. SEVA collection is based on modular vectors that enable the exchangeability of diverse elements (e.g. origins of replication and antibiotic selection markers) and the combination with many cargo sequences for varied end-applications. Firstly, using SEVA vectors containing the broad host range RSF1010 origin we demonstrated that these vectors are replicative not only in model cyanobacteria but also in a new cyanobacterium specie, Chroococcidiopsis sp., which is different from those previously published. Then, we constructed SEVA vectors by harbouring CRISPR elements and showed that they can be easily assimilated not only by conjugation, but also by natural transformation. Finally, we used our SEVA-Cpf1 tools to delete the nblA gene in Synechocystis sp. PCC 6803, demonstrating that our plasmids can be applied for CRISPR-based genome editing technology. Conclusions: The results of this study provide new CRISPR-based vectors based on the SEVA (Standard European Vector Architecture) collection that can improve editing processes using the Cpf1 nuclease in cyanobacteria.

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Engineering cyanobacteria as cell factories for direct trehalose production from CO2

Cited by 33 publications

References 39 publications

Integrative analysis of the salt stress response in cyanobacteria

Integrative analysis of the salt stress response in cyanobacteria

Integrative analysis of the salt stress response in cyanobacteria

SEVA-Cpf1, a CRISPR-Cas12a vector for Genome Editing in Cyanobacteria

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