Engineering Corynebacterium glutamicum for methanol-dependent growth and glutamate production

Tuyishime, Philibert; Wang, Yu; Fan, Liwen; Zhang, Qiongqiong; Li, Qinggang; Zheng, Ping; Sun, Jibin; Ma, Yanhe

doi:10.1016/j.ymben.2018.07.011

Cited by 97 publications

(96 citation statements)

References 44 publications

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“…To fully take advantage of the fusion proteins in vivo, Ru5P generation needs to be efficient enough as not to limit the pathway flux. Metabolic engineered of the pentose phosphate pathway holds promise to address this problem . Notably, recently reported methanol‐dependent synthetic methylotrophs co‐utilized methanol and another carbon source as an Ru5P supplier, such as xylose or gluconate .…”

Section: Resultsmentioning

confidence: 99%

“…Metabolic engineered of the pentose phosphate pathway holds promise to address this problem . Notably, recently reported methanol‐dependent synthetic methylotrophs co‐utilized methanol and another carbon source as an Ru5P supplier, such as xylose or gluconate . Applying the fusion proteins in methanol‐dependent strains may further improve the methanol bioconversion efficiency.…”

Section: Resultsmentioning

confidence: 99%

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Engineering Artificial Fusion Proteins for Enhanced Methanol Bioconversion

et al. 2018

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Methanol is a low‐cost and abundantly available feedstock derived from natural gas and syngas. Although bioconversion holds promise for producing desired chemicals from methanol under economically viable operating conditions, the efficiency is limited by unfavorable kinetics of methanol oxidation and assimilation. Herein, artificial fusion proteins were engineered to enhance methanol bioconversion. Nicotinamide adenine dinucleotide (NAD)‐dependent methanol dehydrogenase (Mdh), 3‐hexulose‐6‐phosphate synthase (Hps) and 6‐phospho‐3‐hexuloisomerase (Phi) from different sources were first screened for catalytic activity. Next, we designed six fusion proteins using the best enzyme candidates and flexible linkers. Fusing Mdh with Hps or Hps‐Phi increased the Vmax of methanol oxidation up to 5.8‐fold, and enhanced methanol conversion to fructose‐6‐phosphate up to 1.3‐fold. Interestingly, fusion engineering changed the polymerization states of proteins and produced larger multimers, which may be responsible for the changed catalytic characteristics. This fusion engineering approach can be coupled with other metabolic engineering strategies for enhanced methanol bioconversion to valuable chemicals.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Engineering Artificial Fusion Proteins for Enhanced Methanol Bioconversion

et al. 2018

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“…In a subsequent study, the functional loss of IolR was shown to result in an upregulation of the gene iolT1 (encoding the myo‐inositol/proton symporter IolT1), and additional reengineering could prove the importance of IolT1 for xylose uptake . Interestingly, the identified mutations are different from those identified by the xylose–methanol ALE study . Altogether, these examples once more highlight the importance of subsequent reengineering of key mutations to expand our knowledge of biological systems.…”

Section: Improving Performance Under Industrial Conditionsmentioning

confidence: 92%

“…If a microbe has no native capacity to consume the particular substrate of interest, targeted engineering can provide an initial, nonoptimal strain, which can subsequently be improved by ALE. This combinatorial strategy was applied in three recent publications that describe improved growth on xylose, cellobiose, and methanol (Table ) …”

Section: Improving Performance Under Industrial Conditionsmentioning

confidence: 99%

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Evolutionary engineering of Corynebacterium glutamicum

Stella

Wiechert

Noack

et al. 2019

Biotechnology Journal

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A unique feature of biotechnology is that we can harness the power of evolution to improve process performance. Rational engineering of microbial strains has led to the establishment of a variety of successful bioprocesses, but it is hampered by the overwhelming complexity of biological systems. Evolutionary engineering represents a straightforward approach for fitness‐linked phenotypes (e.g., growth or stress tolerance) and is successfully applied to select for strains with improved properties for particular industrial applications. In recent years, synthetic evolution strategies have enabled selection for increased small molecule production by linking metabolic productivity to growth as a selectable trait. This review summarizes the evolutionary engineering strategies performed with the industrial platform organism Corynebacterium glutamicum. An increasing number of recent studies highlight the potential of adaptive laboratory evolution (ALE) to improve growth or stress resistance, implement the utilization of alternative carbon sources, or improve small molecule production. Advances in next‐generation sequencing and automation technologies will foster the application of ALE strategies to streamline microbial strains for bioproduction and enhance our understanding of biological systems.

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Chemical Production from Methanol Using Natural and Synthetic Methylotrophs

Chen

Lan

2020

Biotechnology Journal

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Methanol as a chemical feedstock is becoming increasingly important as it is derived from natural gas and is a feasible end-product for captured carbon dioxide. Biological conversion of methanol through natural and synthetic methylotrophs increases the chemical repertoire and is an important direction for one carbon (C1) based chemical economy. Advances in the metabolic engineering and synthetic biology enable development of microbial cell factories for converting methanol into various platform chemicals. In this review, the current status of methanol utilizing microbial factory development is summarized. Also the development of synthetic methylotrophy and methanol-augmented bioproductions is discussed.

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Engineering Corynebacterium glutamicum for methanol-dependent growth and glutamate production

Cited by 97 publications

References 44 publications

Engineering Artificial Fusion Proteins for Enhanced Methanol Bioconversion

Engineering Artificial Fusion Proteins for Enhanced Methanol Bioconversion

Evolutionary engineering of Corynebacterium glutamicum

Chemical Production from Methanol Using Natural and Synthetic Methylotrophs

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