Engineering Copper‐Containing Nanoparticles‐Loaded Silicene Nanosheets with Triple Enzyme Mimicry Activities and Photothermal Effect for Promoting Bacteria‐Infected Wound Healing

Abstract

Skin wounds accompanied by bacterial infections threaten human health, and conventional antibiotic treatments are ineffective for drug‐resistant bacterial infections and chronically infected wounds. The development of non‐antibiotic‐dependent therapeutics is highly desired but remains a challenging issue. Recently, 2D silicene nanosheets with considerable biocompatibility, biodegradability, and photothermal‐conversion performance have received increasing attention in biomedical fields. Herein, copper‐containing nanoparticles‐loaded silicene (Cu2.8O@silicene‐BSA) nanosheets with triple enzyme mimicry catalytic (peroxidase, catalase, and oxidase‐like) activities and photothermal function are rationally designed and fabricated for efficient bacterial elimination, angiogenesis promotion, and accelerated wound healing. Cu2.8O@silicene‐BSA nanosheets display excellent antibacterial activity through synergistic effects of reactive oxygen species generated from multiple catalytic reactions, intrinsic bactericidal activity of released Cu2+ ions, and photothermal effects, achieving high antibacterial efficiencies on methicillin‐resistant Staphylococcus aureus (MRSA) of 99.1 ± 0.7% in vitro and 97.2 ± 1.6% in vivo. In addition, Cu2.8O@silicene‐BSA nanosheets exhibit high biocompatibility for promoting human umbilical vein endothelial cell (HUVEC) proliferation and angiogenic differentiation. In vivo experiments reveal that Cu2.8O@silicene‐BSA nanosheets with synergistic photothermal/chemodynamic therapeutics effectively accelerate MRSA‐infected wound healing by eliminating bacteria, alleviating inflammation, boosting collagen deposition, and promoting angiogenesis. This research presents a promising strategy to engineer photothermal‐assisted nanozyme catalysis for bacteria‐invaded wound healing.

Novel silicene-mesoporous silica nanoparticles conjugated gemcitabine induced cellular apoptosis via upregulating NF-κB p65 nuclear translocation suppresses pancreatic cancer growth in vitro and in vivo

“…The silicene nanosheets (SN) and silicene-MSN (SMSN) nanoparticles were synthesized according to the published literature [26][27][28][29]. Briefly, 20 mg of Gemcitabine was added to the prepared SMSN solution (5 ml, 1 mg ml −1 ), stirred, dispersed, and after 12 h of adsorption, the SMSN-G nanodrug was collected by centrifugation.…”

Gases and gas-releasing materials for the treatment of chronic diabetic wounds

“…MnCoO@PDA/CPH hydrogel 88 Simulate the mechanical and electrical signals of the skin; promote the H 2 O 2 -activated oxidation ability MnCoO@PLE/HA hydrogel 91 Synergistically clear ROS and produce oxygen Ti 3 C 2 @PDA hydrogel 92 Remove excess reactive nitrogen and ROS; promote tissue adhesion and hemostasis PNAs + US hydrogel scaffold 89 Simulate the activity of catalase (CAT) and peroxidase (POD); generate GSH under ultrasound; promote the polarization of macrophages MoS 2 @Au@BSA NSs hydrogel 93 Reduce the ROS to alleviate hypoxia; consume glucose; and eradicate bacteria PBNPs@PLEL hydrogel 94 Reduce the production of ROS; promote angiogenesis; reduce the level of inflammatory factors on the wound Au/Cu 1.6 O/P-C 3 N 5 /Arg@HA hydrogel spray 95 Multienzyme-like activities, including superoxide dismutase (SOD)-, CAT-, glucose oxidase (GOx)-, POD-, and nitric oxide synthase (NOS)-like activities GCNC hydrogel 96 Eliminate ROS; consume glucose; oxygen supply; eradicate bacteria Fe 3 O 4 -Gox nanoparticle 97 Respond to the pH of the wound; shorten inflammatory period; produce a continuous oxygen supply CHA@GOx 98 Simulate CAT and SOD activity; promotes angiogenesis and collagen deposition Catalase analogues (non-nano) Cu 2.8 O@silicene-BSA 99 Simulated catalysis of three enzymes (POD, CAT, and oxidase-like enzyme); antibacterial and anti-inflammatory EPL-g-Fc biodegradable macromolecule 100 Simulate CAT activity; eliminate bacteria EUK-207 mimetic 101 Simulation of SOD and CAT activity CAT agonist PPARβ/δ agonist GW501516 (GW) 102 Activate catalase and reduce ROS in wound; accelerate diabetic wound closure PSeP polysaccharides 103 Increase the activity of CAT and GSH-Px Sodium Aescinate (SA) 104 Increase SOD and CAT activities; reduces inflammation; promote wound contraction SHSG and GTSG carrageenan 105 Inhibit tissue edema; increase the activity of CAT and SOD BMSC + PEG-PU polymer scaffold 106 Reduce oxidative stress; regulate inflammatory mediators; increase the formation of new blood vessels STS gel 107 Increase antioxidant activity; reduce inflammation; increase angiogenesis…”