Engineering controllable biofilms for biotechnological applications

Mukherjee, Manisha; Cao, Bin

doi:10.1111/1751-7915.13715

Cited by 19 publications

(12 citation statements)

References 38 publications

(51 reference statements)

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“…These structures provide to their inhabitants a protective environment in which they can resist harsh conditions such as desiccation, nutrients starvation or the action of toxic compounds [ 2 ]. Microbial biofilms can be considered useful since they are involved in natural biogeochemical cycles and increasingly used in biotechnologies for wastewater treatments or the production of green energies [ 3 ]. However, biofilms also facilitate pathogen persistence despite antimicrobial treatments and thus have a severe negative impact in human health being involved in up to 80% of chronic and recurrent infections [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

The coordinated population redistribution between Bacillus subtilis submerged biofilm and liquid-air pellicle

Sanchez-Vizuete

Dergham

Bridier

et al. 2022

Biofilm

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Bacillus subtilis is a widely used bacterial model to decipher biofilm formation, genetic determinants and their regulation. For several years, studies were conducted on colonies or pellicles formed at the interface with air, but more recent works showed that non-domesticated strains were able to form thick and structured biofilms on submerged surfaces. Taking advantage of time-lapse confocal laser scanning microscopy, we monitored bacterial colonization on the surface and observed an unexpected biphasic submerged biofilm development. Cells adhering to the surface firstly form elongated chains before being suddenly fragmented and released as free motile cells in the medium. This switching coincided with an oxygen depletion in the well which preceded the formation of the pellicle at the liquid-air interface. Residual bacteria still associated with the solid surface at the bottom of the well started to express matrix genes under anaerobic metabolism to build the typical biofilm protruding structures.

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Section: Introductionmentioning

confidence: 99%

The coordinated population redistribution between Bacillus subtilis submerged biofilm and liquid-air pellicle

Sanchez-Vizuete

Dergham

Bridier

et al. 2022

Biofilm

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“…Recently, novel strategies have been developed to create biofilms as artificial platforms for self-assembling functional tags 32 , 34 , 35 . One of these approaches is based on engineering extracellular matrix components, focusing on amyloids as potential targets 8 , 9 , 11 .…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, they are referred as facultative amyloids. These proteins may have multiple domains, some of them involved in biofilm formation 31 , which can be engineered to construct functionalized biofilms for biotechnological purposes 32 . In this work we take advantage of Bap properties to confer programmable functions in the globular and amyloid conformation of the protein.…”

Section: Introductionmentioning

confidence: 99%

Bacterial biofilm functionalization through Bap amyloid engineering

Matilla-Cuenca

Taglialegna

Gil

et al. 2022

npj Biofilms Microbiomes

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Biofilm engineering has emerged as a controllable way to fabricate living structures with programmable functionalities. The amyloidogenic proteins comprising the biofilms can be engineered to create self-assembling extracellular functionalized surfaces. In this regard, facultative amyloids, which play a dual role in biofilm formation by acting as adhesins in their native conformation and as matrix scaffolds when they polymerize into amyloid-like fibrillar structures, are interesting candidates. Here, we report the use of the facultative amyloid-like Bap protein of Staphylococcus aureus as a tool to decorate the extracellular biofilm matrix or the bacterial cell surface with a battery of functional domains or proteins. We demonstrate that the localization of the functional tags can be change by simply modulating the pH of the medium. Using Bap features, we build a tool for trapping and covalent immobilizing molecules at bacterial cell surface or at the biofilm matrix based on the SpyTag/SpyCatcher system. Finally, we show that the cell wall of several Gram-positive bacteria could be functionalized through the external addition of the recombinant engineered Bap-amyloid domain. Overall, this work shows a simple and modulable system for biofilm functionalization based on the facultative protein Bap.

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“…Currently, biofilms are already used to create microbial fuel cells [50], in wastewater treatment [51,52], as biosensors [53,54,54] and in biotechnology [56,57,58,59] (Fig. 1).…”

Section: Discussionmentioning

confidence: 99%