Engineering conduits to resemble natural vascular tissue

Buján, Julia; García‐Honduvilla, Natalio; Bellón, Juan M.

doi:10.1042/ba20030111

Cited by 36 publications

(25 citation statements)

References 137 publications

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“…The study also discovered that successive waves of neutrophils moving towards an infected foreign body became successively less effective in their ability to kill microorganisms. Finally, deposition of adherence proteins, such as albumin, fibrinogen, Hageman factor, high-molecular-weight kininogen, fibronectin, laminin, thrombospondin, collagen and immunoglobulins, on IVC surfaces [33] together with attachment of bacterial cells result in altered IVC surface conditioning, which promotes receptor-mediated attachment of microorganisms. The biomaterials used in IVC manufacture also affect host protein deposition and subsequent bacterial attachment, colonisation, biofilm formation and infections.…”

Section: Effect Of Intravascular Catheter (Ivc) Materials On Ivc-relamentioning

confidence: 99%

Impact of microbial attachment on intravascular catheter-related infections

Zhang

Gowardman

Rickard

2011

International Journal of Antimicrobial Agents

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Intravascular catheters (IVCs) are the most frequently used medical devices in hospitals. However, they are associated with life-threatening IVC-related bloodstream infection (IVC-BSI), which is one of the main hospital-acquired infections, and continue to be associated with morbidity, mortality and additional medical cost. Most published studies focus on measuring the rate of IVC-BSIs and addressing their importance, but only a few studies have mentioned the possible routes for microbes entering the bloodstream, which would help in developing effective prevention methods, and large trial studies are lacking. Some studies on IVC-BSIs have reported the most frequently isolated microbes, but caution needs to be made since many fastidious microbes are not isolated under current laboratory conditions. Although it is known that microbes colonise IVC surfaces and develop biofilms, leading to IVC-BSI, the relationships of microbial biofilms with patients' symptoms or outcomes remain unclear. Here we discuss the knowledge gained from microbial research in other (non-IVC) medical and non-medical applications that may be helpful in understanding the IVC context. In addition, published theory and data regarding microbial colonisation and biofilm development specifically in IVCs are reviewed. More research is needed to explore mechanisms of IVC-BSI and to provide superior prevention strategies.

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Section: Effect Of Intravascular Catheter (Ivc) Materials On Ivc-relamentioning

confidence: 99%

Impact of microbial attachment on intravascular catheter-related infections

Zhang

Gowardman

Rickard

2011

International Journal of Antimicrobial Agents

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“…However, their clinical utility is limited by potential immunogenic responses and the origin of suitable vessels. 2 Recently, synthetic grafts, such as woven poly(ethylene terephthalate) (Dacron) and extended polytetrafluoroethylene, have also been used for vascular reconstruction, but these grafts tend to fail when they are applied to small-diameter vessels because of their poor biocompatibility. 3,4 With the development of tissue engineering, blood vessel tissue engineering has emerged as a promising approach for addressing the shortage of current therapies.…”

Section: Introductionmentioning

confidence: 99%

Electrospun polylactide/silk fibroin–gelatin composite tubular scaffolds for small‐diameter tissue engineering blood vessels

Wang

Zhang

Yin

et al. 2009

J of Applied Polymer Sci

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Many synthetic scaffolds have been used as vascular substitutes for clinical use. However, many of these scaffolds may not show suitable properties when they are exposed to physiologic vascular environments, and they may fail eventually because of some unexpected conditions. Electrospinning technology offers the potential for controlling the composition, structure, and mechanical properties of scaffolds. In this study, a tubular scaffold (inner diameter ¼ 4.5 mm) composed of a polylactide (PLA) fiber outside layer and a silk fibroin (SF)-gelatin fiber inner layer (PLA/SF-gelatin) was fabricated by electrospinning. The morphological, biomechanical, and biological properties of the composite scaffold were examined. The PLA/SF-gelatin composite tubular scaffold possessed a porous structure; the porosity of the scaffold reached 82 AE 2%. The composite scaffold achieved the appropriate breaking strength (1.28 AE 0.21 MPa) and adequate pliability (elasticity up to 41.11 AE 2.17% strain) and possessed a fine suture retention strength (1.07 AE 0.07 N). The burst pressure of the composite scaffold was 111.4 AE 2.6 kPa, which was much higher than the native vessels. A mitochondrial metabolic assay and scanning electron microscopy observations indicated that both 3T3 mouse fibroblasts and human umbilical vein endothelial cells grew and proliferated well on the composite scaffold in vitro after they were cultured for some days. The PLA/ SF-gelatin composite tubular scaffolds presented appropriate characteristics to be considered as candidate scaffolds for blood vessel tissue engineering.

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“…9 The present study demonstrates that ␣ 2 (VIII) collagen is a potent attachment factor for ECs, significantly enhancing EC retention both in vitro and in vivo. In addition, we have identified that EC attachment to recombinant ␣ 2 (VIII) substrata occurs via the ␣ 2 ␤ 1 integrin.…”

Section: Discussionmentioning

confidence: 81%

α ₂ (VIII) Collagen Substrata Enhance Endothelial Cell Retention Under Acute Shear Stress Flow via an α ₂ β ₁ Integrin–Dependent Mechanism

et al. 2006

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Background-Essential to tissue-engineered vascular grafts is the formation of a functional endothelial monolayer capable of resisting the forces of blood flow. This study targeted ␣ 2 (VIII) collagen, a major component of the subendothelial matrix, and examined the ability of and mechanisms by which endothelial cells attach to this collagen under static and dynamic conditions both in vitro and in vivo. Methods and Results-Attachment of human endothelial cells to recombinant ␣ 2 (VIII) collagen was assessed in vitro under static and shear conditions of up to 100 dyne/cm 2 . ␣ 2 (VIII) collagen supported endothelial cell attachment in a dose-dependent manner, with an 18-fold higher affinity for endothelial cells compared with fibronectin. Cell attachment was significantly inhibited by function-blocking anti-␣ 2 (56%) and -␤ 1 (98%) integrin antibodies but was not RGD dependent. Under flow, endothelial cells were retained at significantly higher levels on ␣ 2 (VIII) collagen (53% and 51%) than either fibronectin (23% and 16%) or glass substrata (7% and 1%) at shear rates of 30 and 60 dyne/cm 2 , respectively. In vivo studies, using endothelialized polyurethane grafts, demonstrated significantly higher cell retention rates to ␣ 2 (VIII) collagen-coated than to fibronectin-coated prostheses in the midgraft area (PϽ0.05) after 24 hours' implantation in the caprine carotid artery. Conclusions-These

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Engineering conduits to resemble natural vascular tissue

Cited by 36 publications

References 137 publications

Impact of microbial attachment on intravascular catheter-related infections

Impact of microbial attachment on intravascular catheter-related infections

Electrospun polylactide/silk fibroin–gelatin composite tubular scaffolds for small‐diameter tissue engineering blood vessels

α ₂ (VIII) Collagen Substrata Enhance Endothelial Cell Retention Under Acute Shear Stress Flow via an α ₂ β ₁ Integrin–Dependent Mechanism

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Engineering conduits to resemble natural vascular tissue

Cited by 36 publications

References 137 publications

Impact of microbial attachment on intravascular catheter-related infections

Impact of microbial attachment on intravascular catheter-related infections

Electrospun polylactide/silk fibroin–gelatin composite tubular scaffolds for small‐diameter tissue engineering blood vessels

α 2 (VIII) Collagen Substrata Enhance Endothelial Cell Retention Under Acute Shear Stress Flow via an α 2 β 1 Integrin–Dependent Mechanism

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α ₂ (VIII) Collagen Substrata Enhance Endothelial Cell Retention Under Acute Shear Stress Flow via an α ₂ β ₁ Integrin–Dependent Mechanism