Engineering conductive protein films through nanoscale self-assembly and gold nanoparticles doping

Mejías, Sara H.; López‐Martínez, Elena; Fernandez, Maxence; Couleaud, Pierre; Martín-Lasanta, Ana; Romera, David; Sánchez‐Iglesias, Ana; Casado, Santos; Osorio, Manuel; Abad, José M.; González, M. Teresa; Cortajarena, Aitziber L.

doi:10.1039/d1nr00238d

Cited by 15 publications

(15 citation statements)

References 58 publications

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“…Herein, we have investigated the charge transport phenomenon of the bovine serum albumin (BSA)–Au NCs hybrid as a model system. BSA, a plasma protein, binds and transports a range of hydrophilic molecules readily adsorbed to surfaces, making it a suitable candidate for custom-built electroactive materials. − The hybrid Au-nanostructures were synthesized in an aqueous solution using a straightforward bottom-up approach. By varying the precursor concentration, effective loading of Au nanoclusters per BSA molecule was achieved, which significantly influenced their relative photoluminescence (PL) and time-resolved PL properties.…”

Section: Introductionmentioning

confidence: 99%

Improved Charge Transport across Bovine Serum Albumin–Au Nanoclusters’ Hybrid Molecular Junction

et al. 2022

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Proteins, a highly complex substance, have been an essential element in living organisms, and various applications are envisioned due to their biocompatible nature. Apart from proteins’ biological functions, contemporary research mainly focuses on their evolving potential associated with nanoscale electronics. Here, we report one chemical doping process in model protein molecules (BSA) to modulate their electrical conductivity by incorporating metal (gold) nanoclusters on the surface or within them. The as-synthesized Au NCs incorporated inside the BSA (Au 1 to Au 6) were optically well characterized with UV–vis, time-resolved photoluminescence (TRPL), X-ray photon spectroscopy, and high-resolution transmission electron microscopy techniques. The PL quantum yield for Au 1 is 6.8%, whereas that for Au 6 is 0.03%. In addition, the electrical measurements showed ∼10-fold enhancement of conductivity in Au 6 (8.78 × 10 –3 S/cm), where maximum loading of Au NCs was predicted inside the protein matrix. We observed a dynamic behavior in the electrical conduction of such protein-nanocluster films, which could have real-time applications in preparing biocompatible electronic devices.

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Section: Introductionmentioning

confidence: 99%

Improved Charge Transport across Bovine Serum Albumin–Au Nanoclusters’ Hybrid Molecular Junction

et al. 2022

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“…[40,41] In this work, designed repeat proteins, namely consensus tetratricopeptide repeat proteins (CTPR), were chosen due to their exceptional modular features. [31,32,[42][43][44][45] The 3D structure of CTPR is defined by few conserved residues (9 out of 34), which enables the tailoring of the protein sequence without exerting conformational alterations. Here, CTPR proteins with six repeats and engineered metal coordination sites encoded by cysteines (C6 Cys ) or histidines (C6 His ) were envisaged as optimized environments for the growth of mNCs, which were synthesized following green chemistry premises in a one-pot synthesis procedure.…”

Section: Introductionmentioning

confidence: 99%

An Emerging Nanozyme Class for à la carte Enzymatic‐Like Activities based on Protein‐Metal Nanocluster Hybrids

López‐Domene

Vázquez-Díaz

Modin

et al. 2023

Adv Funct Materials

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In this study, the goal is to fabricate robust and highly efficient peroxidase‐like nanozymes that can ultimately be assembled into films for their easy reuse in catalytic cycles. Nanozymes are designed by mimicking the strategy adopted by metalloproteins to accommodate metal cofactors within their protein structure. The engineered consensus tetratricopeptide repeat (CTPR) protein module is selected as the scaffold to guide the growth and the stabilization of a library of in situ synthesized metal nanoclusters. A deep investigation of the interplay between the composition and function of the nanozymes reveals the impact of the protein templates and nanocluster composition on the peroxidase‐like activity of the hybrids. Moreover, among a total of 24 hybrids, a top‐performing nanozyme results from the growth of Au/Pt bimetallic nanoclusters on a CTPR protein with engineered histidine coordination sites. These nanozymes exhibit improved thermostability and resistance to hydrogen peroxide compared to natural peroxidases like horseradish peroxidase. Finally, it shows the easy fabrication of nanozyme composite films guided throughout the intrinsic self‐assembling properties of the CTPR scaffold. These heterogeneous solid materials are reused in several reaction cycles without significant loss of the catalytic performance, proving these protein‐templated nanozymes as an advantageous alternative to natural enzymes.

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“…Polylactic acid (PLA) is widely used in fields such as biomedicine [ 1 ], packaging coatings [ 2 , 3 ], electronic appliances [ 4 , 5 ], 3D printing [ 6 , 7 ] and a wide range of other applications owing to its excellent biodegradability and mechanical properties. However, its low crystallization ability and slow crystallization rate [ 8 ] limit its wider applications in more fields.…”

Section: Introductionmentioning

confidence: 99%

Effect of Biomass as Nucleating Agents on Crystallization Behavior of Polylactic Acid

et al. 2022

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Polylactic acid (PLA) is one of the most productive biodegradable materials. Its bio-based source makes it truly carbon neutral. However, PLA is hard to crystallize as indicated by a low crystallization rate and a low crystallinity under conventional processing conditions, which limits its wider application. One of the most effective ways to enhance the crystallization ability of PLA is to add nucleating agents. In the context of increasing global environmental awareness and the decreasing reserves of traditional petroleum-based materials, biomass nucleating agents, compared with commonly used petroleum-based nucleating agents, have received widespread attention in recent years due to their abundance, biodegradability and renewability. This paper summarizes the research progress on biomass nucleating agents for regulating the crystallization behavior of polylactic acid. Examples of biomass nucleating agents include cellulose, hemicellulose, lignin, amino acid, cyclodextrins, starch, wood flour and natural plant fiber. Such green components from biomass for PLA are believed to be a promising solution for the development of a wholly green PLA-based system or composites.

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Engineering conductive protein films through nanoscale self-assembly and gold nanoparticles doping

Abstract: We report the fabrication of a conductive biomaterial based on engineered proteins and patterned gold nanoparticles to overcome the challenge of charge transport on macroscopic protein-based materials. This approach has great value for bioelectronics.

Cited by 15 publications

References 58 publications

Improved Charge Transport across Bovine Serum Albumin–Au Nanoclusters’ Hybrid Molecular Junction

Improved Charge Transport across Bovine Serum Albumin–Au Nanoclusters’ Hybrid Molecular Junction

An Emerging Nanozyme Class for à la carte Enzymatic‐Like Activities based on Protein‐Metal Nanocluster Hybrids

Effect of Biomass as Nucleating Agents on Crystallization Behavior of Polylactic Acid

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