Engineering Clostridium acetobutylicum for Enhanced Solvent Production by Overexpression of Pyruvate Decarboxylase from Zymomonas mobilis

Single and multiplexed gene repression in solventogenic Clostridium via Cas12a-based CRISPR interference

“…While a pdc knockout strain has not been characterized in Clostridium , previous studies in C. acetobutylicum have demonstrated high expression of pdc during the switch from acidogenesis to solventogenesis [ 59 ]. Additionally, the expression of heterologous pdc genes in C. acetobutylicum and C. thermocellum has resulted in increased ethanol production in both species, as well as increased acetone and butanol production in C. acetobutylicum [ 60 , 61 ]. In this study, no significant difference in metabolite production, was observed when pdc is targeted on its own via g73 ( Fig.…”