Engineering cell–substrate interactions on porous membranes for microphysiological systems

Allahyari, Zahra; Gaborski, Thomas R.

doi:10.1039/d2lc00114d

Cited by 9 publications

(12 citation statements)

References 77 publications

(223 reference statements)

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“…These parameters affect cell adhesion. It is known that smoother surfaces have less adhesiveness than materials with a rough surface [ 61 , 62 ].…”

Section: Resultsmentioning

confidence: 99%

“…However, most cells with larger micropore sizes (3.0–8.0 µm in diameter) showed a more spherical cell shape with fewer filopodia and lamellipodia, indicating a decrease in the adhesiveness of the material. Zahra Allahyari et al [ 62 ] confirmed in their review that small pores at the nanoscale promote the strongest cell-substrate interactions, while the largest pores at the micron scale prevent these interactions. It is also known that smooth surfaces have weaker adhesion than the surface of a material with moderate porosity.…”

Section: Resultsmentioning

confidence: 99%

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Influence of PHA Substrate Surface Characteristics on the Functional State of Endothelial Cells

Ryltseva

Dudaev

Menzyanova

et al. 2023

JFB

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The needs of modern regenerative medicine for biodegradable polymers are wide and varied. Restoration of the viability of the vascular tree is one of the most important components of the preservation of the usefulness of organs and tissues. The creation of vascular implants compatible with blood is an important task of vascular bioengineering. The function of the endothelial layer of the vessel, being largely responsible for the development of thrombotic complications, is of great importance for hemocompatibility. The development of surfaces with specific characteristics of biomaterials that are used in vascular technologies is one of the solutions for their correct endothelialization. Linear polyhydroxyalkanoates (PHAs) are biodegradable structural polymeric materials suitable for obtaining various types of implants and tissue engineering, having a wide range of structural and physicomechanical properties. The use of PHA of various monomeric compositions in endothelial cultivation makes it possible to evaluate the influence of material properties, especially surface characteristics, on the functional state of cells. It has been established that PHA samples with the inclusion of 3-hydroxyhexanoate have optimal characteristics for the formation of a human umbilical vein endothelial cell, HUVEC, monolayer in terms of cell morphology as well as the levels of expression of vinculin and VE-cadherin. The obtained results provide a rationale for the use of PHA copolymers as materials for direct contact with the endothelium in vascular implants.

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“…These parameters affect cell adhesion. It is known that smoother surfaces have less adhesiveness than materials with a rough surface [ 61 , 62 ].…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Influence of PHA Substrate Surface Characteristics on the Functional State of Endothelial Cells

Ryltseva

Dudaev

Menzyanova

et al. 2023

JFB

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“…Many studies demonstrated the correlation between surface features and the behavior of cells [ 33 , 34 , 35 ]. A recent review provides insight into the importance of membrane pores in controlling cellular responses [ 36 ]. Here, SEM and histological analyses revealed a porous structure that seems to favor the biological activities of both HGF and HOB, demonstrated by the higher expression of functional genes, above all the genes related to ECM components.…”

Section: Discussionmentioning

confidence: 99%

Osteoblasts and Fibroblasts Interaction with a Porcine Acellular Dermal Matrix Membrane

Felice

D’Amico

Pierfelice

et al. 2023

IJMS

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The use of collagen membranes has remained the gold standard in GTR/GBR. In this study, the features and the biological activities of an acellular porcine dermis collagen matrix membrane applicable during dental surgery were investigated, and also by applying hydration with NaCl. Thus, two tested membranes were distinguished, the H-Membrane and Membrane, compared to the control cell culture plastic. The characterization was performed by SEM and histological analyses. In contrast, the biocompatibility was investigated on HGF and HOB cells at 3, 7, and 14 days by MTT for proliferation study; by SEM and histology for cell interaction study; and by RT-PCR for function-related genes study. In HOBs seeded on membranes, mineralization functions by ALP assay and Alizarin Red staining were also investigated. Results indicated that the tested membranes, especially when hydrated, can promote the proliferation and attachment of cells at each time. Furthermore, membranes significantly increased ALP and mineralization activities in HOBs as well as the osteoblastic-related genes ALP and OCN. Similarly, membranes significantly increased ECM-related and MMP8 gene expression in HGFs. In conclusion, the tested acellular porcine dermis collagen matrix membrane, mainly when it is hydrated, behaved as a suitable microenvironment for oral cells.

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“…Focal adhesions, generally located at the cell periphery, anchor ECs to underlying substrates. Their numbers directly correlate with the strength of cell‐matrix interactions, particularly on stiff substrates 45–47 . Relative to nonporous substrates, the disrupted surface geometry of porous glass membranes decreased cell‐substrate contact area, ultimately reducing cell‐matrix interactions and focal adhesion formation.…”

Section: Discussionmentioning

confidence: 99%

Porous honeycomb film membranes enhance endothelial barrier integrity in human vascular wall bilayer model compared to standard track‐etched membranes

Ebrahim

Mwizerwa

Ekwueme

et al. 2023

J Biomedical Materials Res

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In vitro vascular wall bilayer models for drug testing and disease modeling must emulate the physical and biological properties of healthy vascular tissue and its endothelial barrier function. Both endothelial cell (EC)‐vascular smooth muscle cell (SMC) interaction across the internal elastic lamina (IEL) and blood vessel stiffness impact endothelial barrier integrity. Polymeric porous track‐etched membranes (TEM) typically represent the IEL in laboratory vascular bilayer models. However, TEM stiffness exceeds that of diseased blood vessels, and the membrane pore architecture limits EC‐SMC interaction. The mechanical properties of compliant honeycomb film (HCF) membranes better simulate the Young's modulus of healthy blood vessels, and HCFs are thinner (4 vs. 10 μm) and more porous (57 vs. 6.5%) than TEMs. We compared endothelial barrier integrity in vascular wall bilayer models with human ECs and SMCs statically cultured on opposite sides of HCFs and TEMs (5 μm pores) for up to 12 days. Highly segregated localization of tight junction (ZO‐1) and adherens junction (VE‐cadherin) proteins and quiescent F‐actin cytoskeletons demonstrated superior and earlier maturation of interendothelial junctions. Quantifying barrier integrity based on transendothelial electrical resistance (TEER), membranes showed only minor but significant TEER differences despite enhanced junctional protein localization on HCF. Elongated ECs on HCF likely experienced greater paracellular diffusion than blocky ECs on TEM. Also, larger populations of plaques of connexin 43 subunit‐containing gap junctions suggested enhanced EC‐SMC communication across the more porous, thinner HCF. Compared with standard TEMs, engineered vascular wall bilayers cultured on HCFs better replicate physiologic endothelial barrier integrity.

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Engineering cell–substrate interactions on porous membranes for microphysiological systems

Abstract: Microphysiological systems are now widely used to recapitulate physiological and pathological microenvironments in order to study and understand a variety of cellular processes as well as drug delivery and stem...

Cited by 9 publications

References 77 publications

Influence of PHA Substrate Surface Characteristics on the Functional State of Endothelial Cells

Influence of PHA Substrate Surface Characteristics on the Functional State of Endothelial Cells

Osteoblasts and Fibroblasts Interaction with a Porcine Acellular Dermal Matrix Membrane

Porous honeycomb film membranes enhance endothelial barrier integrity in human vascular wall bilayer model compared to standard track‐etched membranes

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