Engineering cell-fluorescent ion track hybrid detectors

Niklas, Martin; Greilich, Steffen; Melzig, Claudius; Akselrod, M.S.; Debus, Jürgen; Jäkel, Oliver; Abdollahi, Amir

doi:10.1186/1748-717x-8-141

Cited by 30 publications

(53 citation statements)

References 13 publications

(23 reference statements)

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“…A549 cells were cultured in DMEM (Biochrom AG) containing 10% FBS as previously described [21]. LLC and H3122 cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) (Biochrom AG).…”

Section: Cell Culture Reagents and Treatmentsmentioning

confidence: 99%

Synergistic effects of crizotinib and radiotherapy in experimental EML4–ALK fusion positive lung cancer

Dai¹,

Wei²,

Schwager³

et al. 2015

Radiotherapy and Oncology

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Section: Cell Culture Reagents and Treatmentsmentioning

confidence: 99%

Synergistic effects of crizotinib and radiotherapy in experimental EML4–ALK fusion positive lung cancer

Dai¹,

Wei²,

Schwager³

et al. 2015

Radiotherapy and Oncology

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“…[4][5][6][7]23 The results presented here also allow LET to be incorporated into analyses of biological experiments in which FNTDs are used as substrates. 4,5,23 The described techniques will allow the biological response to be linked not only to the path of the track but also to the LET of the track. This will potentially enable investigations at the single-cell level on the effect of LET in the DNA damage response, consequently leading to a better understanding of relative biological effectiveness.…”

Section: φ-Letmentioning

confidence: 99%

“…Such measurements have applications in radiobiology experiments in which cells are cultured on the surface of the FNTDs. 4,5 Our aim in our previous work 10,19,20 was to provide an easy-to-use method to measure both LET and absorbed dose in patient-specific fields for the purpose of quality assurance of LET-optimized treatment plans.…”

Section: φ-Letmentioning

confidence: 99%

“…[4][5][6][7] Those experiments involved culturing cells on the surface of the FNTDs, irradiating the FNTD/cell system, transporting it from the beam line, and fixing and immunostaining the cells for imaging with a commercial FCLSM system. [4][5][6][7] The images were then used to extrapolate the trajectory of the track to the position of the cell to infer where the track traversed it.…”

Section: Introductionmentioning

confidence: 99%

“…The experiments described above [4][5][6][7] were done with highlinear energy transfer (LET) carbon and neon ion beams, which produce high ionization densities. Here we report using FNTDs to visualize very low-LET (<3 keV/µm) proton therapy beams, and we demonstrate that FNTDs have the potential to measure LET of individual proton tracks or LET spectra of proton beams.…”

Section: Introductionmentioning

confidence: 99%

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Nanoscale measurements of proton tracks using fluorescent nuclear track detectors

et al. 2016

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Purpose: The authors describe a method in which fluorescence nuclear track detectors (FNTDs), novel track detectors with nanoscale spatial resolution, are used to determine the linear energy transfer (LET) of individual proton tracks from proton therapy beams by allowing visualization and 3D reconstruction of such tracks. Methods: FNTDs were exposed to proton therapy beams with nominal energies ranging from 100 to 250 MeV. Proton track images were then recorded by confocal microscopy of the FNTDs. Proton tracks in the FNTD images were fit by using a Gaussian function to extract fluorescence amplitudes. Histograms of fluorescence amplitudes were then compared with LET spectra. Results: The authors successfully used FNTDs to register individual proton tracks from high-energy proton therapy beams, allowing reconstruction of 3D images of proton tracks along with delta rays. The track amplitudes from FNTDs could be used to parameterize LET spectra, allowing the LET of individual proton tracks from therapeutic proton beams to be determined. Conclusions: FNTDs can be used to directly visualize proton tracks and their delta rays at the nanoscale level. Because the track intensities in the FNTDs correlate with LET, they could be used further to measure LET of individual proton tracks. This method may be useful for measuring nanoscale radiation quantities and for measuring the LET of individual proton tracks in radiation biology experiments. C

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Report of a National Cancer Institute special panel: Characterization of the physical parameters of particle beams for biological research

et al. 2018

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Purpose To define the physical parameters needed to characterize a particle beam in order to allow intercomparison of different experiments performed using different ions at the same facility and using the same ion at different facilities. Methods At the request of the National Cancer Institute (NCI), a special panel was convened to review the current status of the field and to provide suggested metrics for reporting the physical parameters of particle beams to be used for biological research. A set of physical parameters and measurements that should be performed by facilities and understood and reported by researchers supported by NCI to perform pre‐clinical radiobiology and medical physics of heavy ions were generated. Results Standard measures such as radiation delivery technique, beam modifiers used, nominal energy, field size, physical dose and dose rate should all be reported. However, more advanced physical measurements, including detailed characterization of beam quality by microdosimetric spectrum and fragmentation spectra, should also be established and reported. Details regarding how such data should be incorporated into Monte Carlo simulations and the proper reporting of simulation details are also discussed. Conclusions In order to allow for a clear relation of physical parameters to biological effects, facilities and researchers should establish and report detailed physical characteristics of the irradiation beams utilized including both standard and advanced measures. Biological researchers are encouraged to actively engage facility staff and physicists in the design and conduct of experiments. Modeling individual experimental setups will allow for the reporting of the uncertainties in the measurement or calculation of physical parameters which should be routinely reported.

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Engineering cell-fluorescent ion track hybrid detectors

Cited by 30 publications

References 13 publications

Synergistic effects of crizotinib and radiotherapy in experimental EML4–ALK fusion positive lung cancer

Synergistic effects of crizotinib and radiotherapy in experimental EML4–ALK fusion positive lung cancer

Nanoscale measurements of proton tracks using fluorescent nuclear track detectors

Report of a National Cancer Institute special panel: Characterization of the physical parameters of particle beams for biological research

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