Engineering branching morphogenesis using cell communication

Devillard, Chloé D.; Marquette, Christophe A.

doi:10.1016/j.bprint.2023.e00261

Cited by 2 publications

(2 citation statements)

References 98 publications

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“…Fibroblasts can play this role by adopting a pericytic-type phenotype 84 – 86 . To push forward the full understanding of the GCR effect on angiogenesis, it might be necessary, in a close future, to complexify the model, at least toward a multicellular compartment 87 . In addition, it will be essential for future studies to test the toxicity of the antagomirs in in vivo models, since the tissue model utilized for this manuscript is limited for this purpose.…”

Section: Discussionmentioning

confidence: 99%

Space radiation damage rescued by inhibition of key spaceflight associated miRNAs

McDonald,

Kim,

Farmerie

et al. 2024

Nat Commun

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Our previous research revealed a key microRNA signature that is associated with spaceflight that can be used as a biomarker and to develop countermeasure treatments to mitigate the damage caused by space radiation. Here, we expand on this work to determine the biological factors rescued by the countermeasure treatment. We performed RNA-sequencing and transcriptomic analysis on 3D microvessel cell cultures exposed to simulated deep space radiation (0.5 Gy of Galactic Cosmic Radiation) with and without the antagonists to three microRNAs: miR-16-5p, miR-125b-5p, and let-7a-5p (i.e., antagomirs). Significant reduction of inflammation and DNA double strand breaks (DSBs) activity and rescue of mitochondria functions are observed after antagomir treatment. Using data from astronaut participants in the NASA Twin Study, Inspiration4, and JAXA missions, we reveal the genes and pathways implicated in the action of these antagomirs are altered in humans. Our findings indicate a countermeasure strategy that can potentially be utilized by astronauts in spaceflight missions to mitigate space radiation damage.

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Section: Discussionmentioning

confidence: 99%

Space radiation damage rescued by inhibition of key spaceflight associated miRNAs

McDonald,

Kim,

Farmerie

et al. 2024

Nat Commun

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show abstract

“…Fibroblasts can play this role by adopting a pericytic-type phenotype [84][85][86] . To push forward the full understanding of the GCR effect on angiogenesis, it might be necessary, in a close future, to complexify the model, at least toward a multicellular compartment 87 . In addition, it will be essential for future studies to test the toxicity of the antagomirs in in vivo models, since the tissue model utilized for this manuscript is limited for this purpose.…”

Section: Discussionmentioning

confidence: 99%

So Long, and Thanks for All the Antagomirs: Space Radiation Damage Rescued by Inhibition of Key Spaceflight Associated miRNAs

McDonald

Farmerie

Johnson

et al. 2023

Preprint

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From our previous work we have shown a key miRNA signature that is associated with spaceflight can be used as a biomarker and countermeasure to mitigate the damage caused by space radiation. Here, we have further expanded on this work to determine key biological factors that are being rescued by the countermeasure treatment. We performed RNA-sequencing and transcriptomic analysis on our established 3D microvessel cell cultures exposed to simulated deep space radiation (0.5 Gy of Galactic Cosmic Radiation) with and without the antagonists to three microRNAs (i.e. antagomirs). Significant reduction of inflammation and DNA DSBs activity is observed, along with key mitochondria functions being rescued after antagomir treatment. Lastly, we compared the key genes and pathways involved with the antagomirs with astronaut data from Inspiration4 and JAXA missions to demonstrate that the key genes and pathways associated with these experiments occur in humans and this countermeasure strategy can potentially be utilized in astronauts to mitigate the space radiation response.

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Engineering branching morphogenesis using cell communication

Cited by 2 publications

References 98 publications

Space radiation damage rescued by inhibition of key spaceflight associated miRNAs

Space radiation damage rescued by inhibition of key spaceflight associated miRNAs

So Long, and Thanks for All the Antagomirs: Space Radiation Damage Rescued by Inhibition of Key Spaceflight Associated miRNAs

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