Engineering bone tissue using human dental pulp stem cells and an osteogenic collagen-hydroxyapatite-poly (<scp>l</scp>-lactide-co-ɛ-caprolactone) scaffold

Akkouch, Adil; Zhang, Ze; Rouabhia, Mahmoud

doi:10.1177/0885328213486705

Cited by 68 publications

(54 citation statements)

References 39 publications

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“…S1) (8,9). With this validation in hand, we selected human DPSCs as a model system because, as multipotent stem cells, they have the potential to differentiate into a variety of cell types (10)(11)(12)(13). In this way, the diversity of potential phenotypes somewhat matches the input diversity of the cytokine library.…”

Section: Significancementioning

confidence: 99%

“…DPSCs are a type of mesenchymal stem cell (MSC), which have self-renewal and multilineage differentiation potential, including formation of osteoblasts, adipocytes, chondrocytes, muscle cells, and neural cells (12,13,32,33). This multipotency of DPSCs is critical for dental pulp repair and regeneration of teeth.…”

Section: Ifng Is a Primary Checkpoint For Cytokine-induced Odontoblasticmentioning

confidence: 99%

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Interferon-γ is a master checkpoint regulator of cytokine-induced differentiation

Zha

Bucher

Nejatfard

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Cytokines are protein mediators that are known to be involved in many biological processes, including cell growth, survival, inflammation, and development. To study their regulation, we generated a library of 209 different cytokines. This was used in a combinatorial format to study the effects of cytokines on each other, with particular reference to the control of differentiation. This study showed that IFN-γ is a master checkpoint regulator for many cytokines. It operates via an autocrine mechanism to elevate STAT1 and induce internalization of gp130, a common component of many heterodimeric cytokine receptors. This targeting of a receptor subunit that is common to all members of an otherwise diverse family solves the problem of how a master regulator can control so many diverse receptors. When one adds an autocrine mechanism, fine control at the level of individual cells is achieved.A s our understanding of physiology grows, we are increasingly aware of its Newtonian aspects in that for every action there is another reaction with opposite consequences (1). Irrespective of whether one thinks in terms of homeostasis, feedback loops, or checkpoints, we increasingly uncover molecular systems that, in terms of function, induce cells to move in opposite ways. For example, although immune T-cell activation is critical in controlling disease, one needs a checkpoint to guard against overactivity that could result in autoimmunity (2, 3).In addition to adding to our general knowledge of cellular physiology, detailed understanding of the molecular mechanisms of activation and checkpoint processes has important therapeutic implications. Such an understanding allows two separate entry points into the regulation of cellular events. Thus, if one wants to promote a cellular function, the same outcome can be achieved by either enhancing the effector or inhibiting the checkpoint pathways, usually by perturbing the molecules that initiate them.One of the most important physiological systems is the cytokine cascade (4). However, here, because the cascade contains large numbers of separate molecules, each operating through different receptors, simple models of regulation break down. While one can imagine that each cytokine is paired with a separate checkpoint system operating in an opposite direction, it seemed to us to be unlikely, if for no other reason than the economical use of genetic information. Given that one had large sets of related molecules with overlapping signal transduction mechanisms and pathways, it seemed more reasonable to assume that, depending on the circumstance, some members of the cytokine repertoire regulate other members of the repertoire. This differs from situations such as PD-1, where the checkpoint mechanistic cascade differs completely from the activation mechanism. By contrast, we propose that cytokine members of a family regulate each other by perturbing common molecular mechanisms. Thus, if gene action economy is proposed for a family, its hallmark should be that some of the same molecules used for...

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Section: Significancementioning

confidence: 99%

Section: Ifng Is a Primary Checkpoint For Cytokine-induced Odontoblasticmentioning

confidence: 99%

Interferon-γ is a master checkpoint regulator of cytokine-induced differentiation

Zha

Bucher

Nejatfard

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…Nowadays, there are many natural and synthetic hydrogel which can be used as a scaffold, include collagen/gelatin, chitosan, chondroitin sulfate, hyaluronic acid, agar/agarose, fibrin [4], alginate [5], and hydroxyapatite [6]. Among all of these materials, chitosan is preferable because of its abundance, biocompatibility, biodegradability, and its degradation products did not harmful to the body [7].…”

Section: Introductionmentioning

confidence: 99%

Significance of Glucose Addition on Chitosan-Glycerophosphate Hydrogel Properties

2018

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“…Os estudos mais antigos já relatavam a biocompatibilidade do material e sua aplicabilidade envolvendo a ortopedia (Heimke, 1989) e a odontologia (Deeb et al, 1988). E, até os dias atuais, a hidroxiapatita é muito utilizada para recuperação de tecidos mineralizados (Akkouch et al, 2013).…”

Section: Hidroxiapatita E Suas Nanopartículasunclassified

“…A hidroxiapatita associada a outros materiais promove a diferenciação óssea a partir de osteoblastos derivados de células-tronco de polpa dental (Akkouch et al, 2013). Akkouch et al (2013) realizaram um estudo com o objetivo de projetar uma nova matriz para aplicação em implantes ósseos. Essa matriz era composta por hidroxiapatita, colágeno e um compósito denominado poly (l-lactide-co-ε-caprolactone).…”

Section: Hidroxiapatita E Suas Nanopartículasunclassified

Comparação da dinâmica da resposta celular utilizando dois diferentes biomateriais: Nanopartículas de Hidroxiapatita e Agregado Trióxido Mineral (MTA)

Berti¹

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Engineering bone tissue using human dental pulp stem cells and an osteogenic collagen-hydroxyapatite-poly (l-lactide-co-ɛ-caprolactone) scaffold

Cited by 68 publications

References 39 publications

Interferon-γ is a master checkpoint regulator of cytokine-induced differentiation

Interferon-γ is a master checkpoint regulator of cytokine-induced differentiation

Significance of Glucose Addition on Chitosan-Glycerophosphate Hydrogel Properties

Comparação da dinâmica da resposta celular utilizando dois diferentes biomateriais: Nanopartículas de Hidroxiapatita e Agregado Trióxido Mineral (MTA)

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