Abstract:Although skeletal muscle is highly regenerative following injury or
disease, endogenous self-regeneration is severely impaired in conditions of
volume traumatic muscle loss. Consequently, tissue engineering approach is a
promising approach to regenerate skeletal muscle. Biological scaffolds serve as
not only structural support for the promotion of cellular ingrowth, but they
also impart potent modulatory signaling cues that may be beneficial for tissue
regeneration. In this work, the progress of tissue enginee… Show more
“…This can also been see in the woven pattern in Figure where fibers were hand assembled without any visible damage or deformation. Given the dimensions of the generated fibers, the presented method could be useful in many fields, for example, for generating conduits for peripheral nerve regeneration (Dixon et al, ), skeletal muscle (Nakayama, Shayan, & Huang, ) or tendon (Lovati, Bottagisio, & Moretti, ). By assembling fibers as demonstrated in Figure , possibly with different cell types and sizes, other constructs as, for example, cardiac and skin patches can be obtained.…”
Tissue‐engineered constructs have great potential in many intervention strategies. In order for these constructs to function optimally, they should ideally mimic the cellular alignment and orientation found in the tissues to be treated. Here we present a simple and reproducible method for the production of cell‐laden pure fibrin micro‐fibers with longitudinal topography. The micro‐fibers were produced using a molding technique and longitudinal topography was induced by a single initial stretch. Using this method, fibers up to 1 m in length and with diameters of 0.2–3 mm could be produced. The micro‐fibers were generated with embedded endothelial cells, smooth muscle cell/fibroblasts or Schwann cells. Polarized light and scanning electron microscopy imaging showed that the initial stretch was sufficient to induce longitudinal topography in the fibrin gel. Cells in the unstretched control micro‐fibers elongated randomly in both the floating and encapsulated environments, whereas the cells in the stretched micro‐fibers responded to the introduced topography by adopting a similar orientation. Proof of concept bottom‐up tissue engineering (TE) constructs are shown, all displaying various anisotropic organization of cells within. This simple, economical, versatile and scalable approach for the production of highly orientated and cell‐laden micro‐fibers is easily transferrable to any TE laboratory.
“…This can also been see in the woven pattern in Figure where fibers were hand assembled without any visible damage or deformation. Given the dimensions of the generated fibers, the presented method could be useful in many fields, for example, for generating conduits for peripheral nerve regeneration (Dixon et al, ), skeletal muscle (Nakayama, Shayan, & Huang, ) or tendon (Lovati, Bottagisio, & Moretti, ). By assembling fibers as demonstrated in Figure , possibly with different cell types and sizes, other constructs as, for example, cardiac and skin patches can be obtained.…”
Tissue‐engineered constructs have great potential in many intervention strategies. In order for these constructs to function optimally, they should ideally mimic the cellular alignment and orientation found in the tissues to be treated. Here we present a simple and reproducible method for the production of cell‐laden pure fibrin micro‐fibers with longitudinal topography. The micro‐fibers were produced using a molding technique and longitudinal topography was induced by a single initial stretch. Using this method, fibers up to 1 m in length and with diameters of 0.2–3 mm could be produced. The micro‐fibers were generated with embedded endothelial cells, smooth muscle cell/fibroblasts or Schwann cells. Polarized light and scanning electron microscopy imaging showed that the initial stretch was sufficient to induce longitudinal topography in the fibrin gel. Cells in the unstretched control micro‐fibers elongated randomly in both the floating and encapsulated environments, whereas the cells in the stretched micro‐fibers responded to the introduced topography by adopting a similar orientation. Proof of concept bottom‐up tissue engineering (TE) constructs are shown, all displaying various anisotropic organization of cells within. This simple, economical, versatile and scalable approach for the production of highly orientated and cell‐laden micro‐fibers is easily transferrable to any TE laboratory.
“…Moreover, different cell lineages, namely mouse Mabs and human primary myoblast, could react differently to the bioink mechano-chemical properties as extensively demonstrated for other myogenic stem/progenitor cells [40,41]. Hence, we performed a preliminary set of in vitro experiments with the sole intent of demonstrating the compatibility of the proposed approach with primary human myoblasts (hMyob) and verifying cell stability in terms of myogenic differentiation capacity.…”
Section: In Vitro Characterization Of Human-derived Myo-substitutesmentioning
The importance of skeletal muscle tissue is undoubted being the controller of several vital functions including respiration and all voluntary locomotion activities. However, its regenerative capability is limited and significant tissue loss often leads to a chronic pathologic condition known as volumetric muscle loss. Here, we propose a biofabrication approach to rapidly restore skeletal muscle mass, 3D histoarchitecture and functionality. By recapitulating muscle anisotropic organization at the microscale level, we demonstrate to efficiently guide cell differentiation and myobundle formation both in vitro and in vivo. Of note, upon implantation, the biofabricated myosubstitutes support the formation of new blood vessels and neuromuscular junctions -pivotal aspects for cell survival and muscle contractile functionalities -together with an advanced along with muscle mass and force recovery. Together, these data represent a solid base for further testing the myo-substitutes in large animal size and a promising platform to be eventually translated into clinical scenarios.
“…48,49 Decellularized scaffolds are considered to offer the most potential in the immediate future because they are available off the shelf and have a potentially easier regulatory pathway if not supplemented by cells or growth factors. 50 Engineered muscle cells have been studied in animal models. They have to be cultured for several weeks, making their use in acute injuries impractical.…”
Muscle strains occur frequently in recreational and professional sports. This article considers various treatment options in a biological context and reviews evidence of their efficacy. Treatments reviewed include the PRICE principle (Protection, Rest, Ice, Compression, Elevation), early mobilization, physical therapy, hematoma aspiration, platelet-rich plasma injections, use of nonsteroidal anti-inflammatory drugs, corticosteroids, and local anesthetics, cellular therapies, and surgery.
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