Engineering Biocatalytic and Biosorptive Materials for Environmental Applications

Zhu, Baotong; Chen, Yingying; Wei, Na

doi:10.1016/j.tibtech.2018.11.005

Cited by 44 publications

(25 citation statements)

References 95 publications

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“…These contaminants are biologically active and potentially harmful to human health and ecosystems, and thus there is a critical need to develop advanced treatment processes in water reclamation (Boxall et al, ; Gilbert, ; Hughes, Kay, & Brown, ; Kolpin et al, ). Enzyme biocatalysis, which uses biological enzymes to facilitate beneficial chemical transformations, has received increasing attention recently as a green chemistry alternative for industrial, biomedical, agricultural, and environmental applications (Zhu, Chen, & Wei, ). Particularly, enzyme biocatalysis holds great promise as a sustainable approach for degradation of persistent organic contaminants due to its advantages including high activity under ambient conditions, little generation of toxic byproducts, and low energy requirement (Dvorak, Bidmanova, Damborsky, & Prokop, ; Eibes, Arca‐Ramos, Feijoo, Lema, & Moreira, ; Hutchison, Guest, & Zilles, ; Hutchison et al, ; Schmid et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…A novel class of cell surface display enzyme biocatalysts has emerged with the advancement of synthetic biology technology to address the challenges of existing enzyme immobilization systems (Chen, Stemple, Kumar, & Wei, ; Zhu, Chen et al, ; Zhu & Wei, ). We have constructed a surface display laccase (SDL) biocatalyst, where the laccase from white‐rot fungi Trametes sp.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Biocatalytic properties of cell surface display laccase for degradation of emerging contaminant acetaminophen in water reclamation

Chen

Wei

2019

Biotech & Bioengineering

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The surface display laccase (SDL) biocatalyst, where the enzyme laccase is displayed on the surface of biological cells through synthetic biology, provides a new opportunity to develop sustainable technologies for removal of emerging contaminants from wastewater. This study vigorously characterized biocatalytic properties of the SDL in comparison to free laccase in removing emerging contaminant acetaminophen (APAP), with the aim to understand the effect of surface display on enzyme functionality and identify the strategy to overcome the potential limitation. The SDL could effectively remove APAP. Adding redox mediators substantially improved the removal efficiency. The Michaelis–Menten kinetic analysis showed that the redox mediator 2,2‐azinobis‐3‐ethylbenzothiazoline‐6‐sulfonate could overcome the limitation of APAP accessing the active site of laccase in the SDL biocatalyst. The APAP removal rate catalyzed by the SDL in real secondary wastewater effluent was higher than that in acetate buffer; comprehensive enzyme kinetic analysis provided clear evidence that there were redox mediating compounds in the wastewater. Analysis of transformation products revealed that surface display did not change laccase functionality in terms of APAP transformation mechanism. In addition, the SDL retained 88% of the initial activity after six repeated APAP biotransformation reactions. Results from this study provide a scientific basis for developing and implementing SDL as an innovative biocatalytic material for contaminant treatment applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biocatalytic properties of cell surface display laccase for degradation of emerging contaminant acetaminophen in water reclamation

Chen

Wei

2019

Biotech & Bioengineering

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“…A successful enzyme biocatalytic system for real-world applications requires high efficiency, robustness, and reuse/regeneration of the biocatalysts. It is far from an economical option to directly use free enzymes in large-scale reactions due to the relatively short enzyme lifetimes and difficulty in enzyme recovery and reuse [105]. Engineering whole-cell biocatalysts constantly producing functional plastic-degrading enzymes could be a strategy to overcome the problem of short enzyme lifetimes [106,107].…”

Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 99%

Enzyme discovery and engineering for sustainable plastic recycling

Zhu

Wang

Wei

2022

Trends in Biotechnology

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The drastically increasing amount of plastic waste is causing an environmental crisis that requires innovative technologies for recycling post-consumer plastics to achieve waste valorization while meeting environmental quality goals. Biocatalytic depolymerization mediated by enzymes has emerged as an efficient and sustainable alternative for plastic treatment and recycling. A variety of plasticdegrading enzymes have been discovered from microbial sources. Meanwhile, protein engineering has been exploited to modify and optimize plastic-degrading enzymes. This review highlights the recent trends and up-to-date advances in mining novel plastic-degrading enzymes through state-of-the-art omics-based techniques and improving the enzyme catalytic efficiency and stability via various protein engineering strategies. Future research prospects and challenges are also discussed. Biocatalysis as an Emerging Solution for the Global Plastic Waste ChallengePlastic materials play a revolutionary role in the modern world, although the enormous manufacture and extensive use of plastic commodities inevitably generate an extraordinary amount of post-consumer plastic waste. Around 12 000 million metric tons of plastic waste are predicted to accumulate in landfills and the natural environment by 2050 [1]. Improper handling of plastic waste has caused a grand environmental challenge. The debris of plastic waste, especially microplastics (see Glossary), can impose hazardous effects on various organisms and eventually threaten human well-being [2-5]. In addition, the degradation resistance of plastics further escalates their adverse environmental impacts [6]. Therefore, it is urgent to develop innovative technologies for treatment and recycling of post-consumer plastics, to achieve both waste valorization and environmental protection.Enzymatic biocatalysis has gained increasing attention as an eco-friendly alternative to conventional plastic treatment and recycling methods (Box 1) [7]. To date, various microbial plastic-degrading enzymes have been discovered, representing promising biocatalyst candidates for plastic depolymerization. Considering the ubiquity of plastics in different ecosystems and the tremendous metabolic and genetic diversity of microorganisms, microbial communities in various habitats have likely evolved capabilities in plastic decomposition and utilization. The plastic-degrading enzymes identified so far might only account for a small portion of the enzymes relevant to plastic depolymerization in the environment. Therefore, it is of ever-growing interest to explore diverse environments to discover new plastic-degrading enzymes with desirable properties and functionalities. However, naturally occurring plasticdegrading enzymes are not well suited for synthetic plastic degradation in industrial applications due to poor thermostability and low catalytic activity. Particularly, synthetic plastic materials usually possess distinct physical and chemical properties (e.g., high crystallinity) that render them more resistant to...

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“…SDG #7, 11, 12, 13: Synthetic biologists are engineering organisms to manufacture vital resources, including biofuels, pigments, drug precursors, biodegradable plastics, and "smart" materials which are able to self-assemble and selfrepair (Zhou et al 2018;Darvishi et al 2018;Le Feuvre & Scrutton 2018). SDG #8, 9, 11: Biocatalysts can convert greenhouse gases from industrial processes into valuable products like rubber and feedstock chemicals (Newlight 2020;BIO 2013;Zhu et al 2019). Compared with chemical catalysts, biocatalysts produce high-value chemicals at higher yields with reduced costs and waste.…”

Section: Introductionmentioning

confidence: 99%

Invest in Canadian Synthetic Biology to Meet Commitments to Sustainable Development and Support Economic Recovery

Grue¹,

Hamadache²,

Maddiboina³

et al. 2021

JSPG

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Canadian post-COVID-19 economic recovery efforts have been framed around values aligned with Canada's commitments to the United Nations Sustainable Development Goals (SDGs), primarily concerning environmental sustainability. The field of synthetic biology (synbio) offers many innovative ways to achieve these goals while growing the economy. Here, we discuss the opportunity for Canada to become a leader in clean technology applications of synbio. Investments in synthetic biology, which has traditionally been underfunded compared to other countries, will have beneficial impacts on the environment while driving Canada's post-pandemic economic recovery.

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Engineering Biocatalytic and Biosorptive Materials for Environmental Applications

Cited by 44 publications

References 95 publications

Biocatalytic properties of cell surface display laccase for degradation of emerging contaminant acetaminophen in water reclamation

Biocatalytic properties of cell surface display laccase for degradation of emerging contaminant acetaminophen in water reclamation

Enzyme discovery and engineering for sustainable plastic recycling

Invest in Canadian Synthetic Biology to Meet Commitments to Sustainable Development and Support Economic Recovery

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