Engineering bacteria-seaweed symbioses for modulating the photosynthate content of Ulva (Chlorophyta): Significant for the feedstock of bioethanol production

Polikovsky, Mark; Califano, Gianmaria; Dunger, Nico; Wichard, Thomas; Golberg, Alexander

doi:10.1016/j.algal.2020.101945

Cited by 28 publications

(11 citation statements)

References 59 publications

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“…It should also be instrumental in studying the dynamic exchange of thallusin precursors and the active substance between algae and bacteria. As thallusin contributes to algal growth promotion, it may be used as an effective non-gene disruptive strategy for sustainable algal biorefineries [44] and a modulator of production rates of secondary metabolites [45,46].…”

Section: Discussionmentioning

confidence: 99%

Thallusin Quantification in Marine Bacteria and Algae Cultures

et al. 2022

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Thallusin, a highly biologically active, phytohormone-like and bacterial compound-inducing morphogenesis of the green tide-forming macroalga Ulva (Chlorophyta), was determined in bacteria and algae cultures. A sensitive and selective method was developed for quantification based on ultra-high-performance liquid chromatography coupled with electrospray ionization and a high-resolution mass spectrometer. Upon C18 solid phase extraction of the water samples, thallusin was derivatized with iodomethane to inhibit the formation of Fe–thallusin complexes interfering with the chromatographic separation. The concentration of thallusin was quantified during the relevant phases of the bacterial growth of Maribacter spp., ranging from 0.16 ± 0.01 amol cell−1 (at the peak of the exponential growth phase) to 0.86 ± 0.13 amol cell−1 (late stationary phase), indicating its accumulation in the growth medium. Finally, we directly determined the concentration of thallusin in algal culture to validate our approach for monitoring applications. Detection and quantification limits of 2.5 and 7.4 pmol L−1, respectively, were reached, which allow for quantifying ecologically relevant thallusin concentrations. Our approach will enable the surveying of thallusin in culture and in nature and will thus contribute to the chemical monitoring of aquaculture.

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Section: Discussionmentioning

confidence: 99%

Thallusin Quantification in Marine Bacteria and Algae Cultures

et al. 2022

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“…Ulva is a valuable marine resource, and its biomass can be used for a lot of purposes (see above). However, its biochemical composition varies significantly according to the strains, species, environmental conditions, and likely other factors, such as its associated microbiome [ 19 , 32 , 35 , 115 , 116 ]. Environmental growth conditions are thought to be the main factor influencing the composition of seaweed [ 117 ].…”

Section: Strategies To Improve Ulva Biomass Yield ...mentioning

confidence: 99%

“…While studies have identified the bacteria required for Ulva development [ 1 , 150 , 151 ], studies identifying specific bacteria influencing the growth of mature thallus and the biochemical composition of the biomass are still lacking. To date, a limited number of studies have attempted to demonstrate that certain bacteria can promote Ulva growth [ 167 , 168 ] and can affect the biochemical composition of Ulva [ 116 ]. Further, examination of the molecular mechanisms driving Ulva : microbial interactions is still limited.…”

Section: Strategies To Improve Ulva Biomass Yield ...mentioning

confidence: 99%

Applications of Ulva Biomass and Strategies to Improve Its Yield and Composition: A Perspective for Ulva Aquaculture

Simón

McHale

Sulpice

2022

Biology

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Sea lettuce (Ulva spp.), with its worldwide distribution and remarkable ability to grow rapidly under various conditions, represents an important natural resource that is still under-exploited. Its biomass can be used for a wide range of applications in the food/feed, pharmaceutical, nutraceutical, biofuel, and bioremediation industries. However, knowledge of the factors affecting Ulva biomass yield and composition is far from complete. Indeed, the respective contributions of the microbiome, natural genetic variation in Ulva species, environmental conditions and importantly, the interactions between these three factors on the Ulva biomass, have been only partially elucidated. Further investigation is important for the implementation of large-scale Ulva aquaculture, which requires stable and controlled biomass composition and yields. In this review, we document Ulva biomass composition, describe the uses of Ulva biomass and we propose different strategies for developing a sustainable and profitable Ulva aquaculture industry.

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“…Consequently, microbiome engineering can be considered to address fundamental research questions and to increase algal productivity. The first applications of microbiome engineering under laboratory conditions demonstrated that the photosynthates (e.g., glycerol) are changing with the microbiome (Polikovsky et al, 2020).…”

Section: Perspectives In Microbiome Engineeringmentioning

confidence: 99%

Microbiome-Dependent Adaptation of Seaweeds Under Environmental Stresses: A Perspective

2020

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The microbiome of macroalgae facilitates their adaptation to environmental stress. As bacteria release algal growth and morphogenesis promoting factors (AGMPFs), which are necessary for the healthy development of macroalgae, bacteria play a crucial role in stress adaptation of bacterial-algal interactions. To better understand the level of macroalgal dependence on the microbiome under various stress factors such as light, temperature, salt, or micropollutants, we propose a reductionist analysis of a tripartite model system consisting of the axenic green alga Ulva (Chlorophyta) re-infected with two essential bacteria. This analysis will allow us to decipher the stress response of each symbiont within this cross-kingdom interaction. The paper highlights studies on possible survival strategies embedded in cross-kingdom interactions that govern the stress adaptation, including general features of metabolic pathways in the macroalgal host or more specific features such as alterations in the composition and/or diversity of bacterial assemblages within the microbiome community. Additionally, we present some preliminary results regarding the effect of recently isolated bacteria from the Potter Cove, King George Island (Isla 25 de Mayo) in Antarctica, on the model system Ulva mutabilis Føyn purified gametes. The results indicate that cold-adapted bacteria release AGMPFs, inducing cell differentiation, and cell division in purified cultures. We propose that microbiome engineering can be used to increase the adaptability of macroalgae to stressful situations with implications for, e.g., the sustainable management of (land-based) aquaculture systems.

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Engineering bacteria-seaweed symbioses for modulating the photosynthate content of Ulva (Chlorophyta): Significant for the feedstock of bioethanol production

Cited by 28 publications

References 59 publications

Thallusin Quantification in Marine Bacteria and Algae Cultures

Thallusin Quantification in Marine Bacteria and Algae Cultures

Applications of Ulva Biomass and Strategies to Improve Its Yield and Composition: A Perspective for Ulva Aquaculture

Microbiome-Dependent Adaptation of Seaweeds Under Environmental Stresses: A Perspective

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