Engineering Approaches for Creating Skeletal Muscle

Vogt, Caleb; Tahtinen, Mitchell; Zhao, Feng

doi:10.1142/9789813149199_0001

Cited by 3 publications

(4 citation statements)

References 71 publications

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“…Tissue engineering (TE) and regenerative medicine are promising approaches to tackle the need for alternative muscle-repair strategies [14,15]. The aims to create functional tissue constructs by combining cells, biomaterials, and biologically active molecules [13,16] as well as methods of seeding and growing muscle cells in 2D or on biomaterial scaffolds have been demonstrated [17][18][19][20][21][22][23][24]. It has been possible to improve the proliferation, alignment, and differentiation of muscle cells by matching biomaterial scaffolds of defined geometries, surface properties, porosities, and mechanical properties [25].…”

Section: Introductionmentioning

confidence: 99%

“…It has been possible to improve the proliferation, alignment, and differentiation of muscle cells by matching biomaterial scaffolds of defined geometries, surface properties, porosities, and mechanical properties [25]. An alternative strategy is the encapsulation of cells inside three-dimensional (3D) hydrogels [19,20]. As an attempt to create 3D muscle tissue constructs with similar complexity as native tissue, additive manufacturing-based biofabrication may be a key route for in-vitro muscle engineering [26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

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3D printed oxidized alginate-gelatin bioink provides guidance for C2C12 muscle precursor cell orientation and differentiation via shear stress during bioprinting

et al. 2020

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Biofabrication can be a tool to three-dimensionally (3D) print muscle cells embedded inside hydrogel biomaterials, ultimately aiming to mimic the complexity of the native muscle tissue and to create in-vitro muscle analogues for advanced repair therapies and drug testing. However, to 3D print muscle analogues of high cell alignment and synchronous contraction, the effect of biofabrication process parameters on myoblast growth has to be understood. A suitable biomaterial matrix is required to provide 3D printability as well as matrix degradation to create space for cell proliferation, matrix remodelling capacity, and cell differentiation. We demonstrate that by the proper selection of nozzle size and extrusion pressure, the shear stress during extrusion-bioprinting of mouse myoblast cells (C2C12) can achieve cell orientation when using oxidized alginate-gelatin (ADA-GEL) hydrogel bionk. The cells grow in the direction of printing, migrate to the hydrogel surface over time, and differentiate into ordered myotube segments in areas of high cell density. Together, our results show that ADA-GEL hydrogel can be a simple and cost-efficient biodegradable bioink that allows the successful 3D bioprinting and cultivation of C2C12 cells in-vitro to study muscle engineering.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

3D printed oxidized alginate-gelatin bioink provides guidance for C2C12 muscle precursor cell orientation and differentiation via shear stress during bioprinting

et al. 2020

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“…Replicating the highly aligned architecture of skeletal muscle is also very important in guiding myotube alignment during myogenesis [ 48 ]. Many approaches have been used to fabricate aligned substrates for cells to grow along, the most common of which are electrospinning, 3D printing, and micromolding.…”

Section: Properties That Are Desirable For Skeletal Muscle Regeneratimentioning

confidence: 99%

Advances in biomaterials for skeletal muscle engineering and obstacles still to overcome

Smoak

Mikos

2020

Materials Today Bio

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Repair of injured skeletal muscle is a sophisticated process that uses immune, muscle, perivascular, and neural cells. In acute injury, the robust endogenous repair process can facilitate complete regeneration with little to no functional deficit. However, in severe injury, the damage is beyond the capacity for self-repair, often resulting in structural and functional deficits. Aside from the insufficiencies in muscle function, the aesthetic deficits can impact quality of life. Current clinical treatments are significantly limited in their capacity to structurally and functionally repair the damaged skeletal muscle. Therefore, alternative approaches are needed. Biomaterial therapies for skeletal muscle engineering have leveraged natural materials with sophisticated scaffold fabrication techniques to guide cell infiltration, alignment, and differentiation. Advances in biomaterials paired with a standardized and rigorous assessment of resulting tissue formation have greatly advanced the field of skeletal muscle engineering in the last several years. Herein, we discuss the current trends in biomaterials-based therapies for skeletal muscle regeneration and present the obstacles still to be overcome before clinical translation is possible. With millions of people affected by muscle trauma each year, the development of a therapy that can repair the structural and functional deficits after severe muscle injury is pivotal.

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“…Approaches using micropatterning techniques to regulate cell alignment have been found to be effective in mimicking muscle tissue structure, composition, and function (Nakamoto et al, 2014;Xiang et al, 2022). Such materials have demonstrated the ability to induce muscle cell alignment, promote myogenic differentiation at early stages for cell fusion, and develop long and thick myotubes due to their morphological and topographical characteristics (Liu et al, 2017;Vogt et al, 2017;Bloise et al, 2018;Narayanan et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Bioactive cellulose acetate nanofiber loaded with annatto support skeletal muscle cell attachment and proliferation

Santos

Cotta

Santos

et al. 2023

Front. Bioeng. Biotechnol.

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Electrospinning emerged as a promising technique to produce scaffolds for cultivated meat in function of its simplicity, versatility, cost-effectiveness, and scalability. Cellulose acetate (CA) is a biocompatible and low-cost material that support cell adhesion and proliferation. Here we investigated CA nanofibers, associated or not with a bioactive annatto extract (CA@A), a food-dye, as potential scaffolds for cultivated meat and muscle tissue engineering. The obtained CA nanofibers were evaluated concerning its physicochemical, morphological, mechanical and biological traits. UV-vis spectroscopy and contact angle measurements confirmed the annatto extract incorporation into the CA nanofibers and the surface wettability of both scaffolds, respectively. SEM images revealed that the scaffolds are porous, containing fibers with no specific alignment. Compared with the pure CA nanofibers, CA@A nanofibers showed increased fiber diameter (420 ± 212 nm vs. 284 ± 130 nm). Mechanical properties revealed that the annatto extract induces a reduction of the stiffness of the scaffold. Molecular analyses revealed that while CA scaffold favored C2C12 myoblast differentiation, the annatto-loaded CA scaffold favored a proliferative state of these cells. These results suggest that the combination of cellulose acetate fibers loaded with annatto extract may be an interesting economical alternative for support long-term muscle cells culture with potential application as scaffold for cultivated meat and muscle tissue engineering.

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Engineering Approaches for Creating Skeletal Muscle

Cited by 3 publications

References 71 publications

3D printed oxidized alginate-gelatin bioink provides guidance for C2C12 muscle precursor cell orientation and differentiation via shear stress during bioprinting

3D printed oxidized alginate-gelatin bioink provides guidance for C2C12 muscle precursor cell orientation and differentiation via shear stress during bioprinting

Advances in biomaterials for skeletal muscle engineering and obstacles still to overcome

Bioactive cellulose acetate nanofiber loaded with annatto support skeletal muscle cell attachment and proliferation

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