Engineering a multifunctional 3D-printed PLA-collagen-minocycline-nanoHydroxyapatite scaffold with combined antimicrobial and osteogenic effects for bone regeneration

Martín, Víctor; Ribeiro, Isabel; Alves, Marta M.; Gonçalves, Lídia; Cláudio, R.A.; Grenho, Liliana; Fernandes, Maria Helena; Gomes, Pedro; Santos, Catarina; Bettencourt, Ana

doi:10.1016/j.msec.2019.03.056

Cited by 140 publications

(101 citation statements)

References 57 publications

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“…Currently, intensive work is underway on the possibility of 3D printing composite materials. This technique involves the design of customized structures and enables effective filling of bone defects [98]. Additive manufacturing (AM) is another rapidly growing area, which includes 3D printing using computer technology (CAD).…”

Section: New Trendsmentioning

confidence: 99%

Biologically Inspired Collagen/Apatite Composite Biomaterials for Potential Use in Bone Tissue Regeneration—A Review

2020

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Type I collagen and nanocrystalline-substituted hydroxyapatite are the major components of a natural composite—bone tissue. Both of these materials also play a significant role in orthopedic surgery and implantology; however, their separate uses are limited; apatite is quite fragile, while collagen’s mechanical strength is very poor. Therefore, in biomaterial engineering, a combination of collagen and hydroxyapatite is used, which provides good mechanical properties with high biocompatibility and osteoinduction. In addition, the porous structure of the composites enables their use not only as bone defect fillers, but also as a drug release system providing controlled release of drugs directly to the bone. This feature makes biomimetic collagen–apatite composites a subject of research in many scientific centers. The review focuses on summarizing studies on biological activity, tested in vitro and in vivo.

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Section: New Trendsmentioning

confidence: 99%

Biologically Inspired Collagen/Apatite Composite Biomaterials for Potential Use in Bone Tissue Regeneration—A Review

2020

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“…51,52 Opn was the downstream target of Runx2 and regulated matrix mineralization. 48,53 Osx, an essential transcription factor for osteoblastic differentiation, played a crucial role in the differentiation and maturation of osteoblasts. 18 During the bone formation, BMP-2 or one of its co-signaling BMPs is imperative.…”

Section: Pcr Analysis and Western Blot Analysismentioning

confidence: 99%

<p>Effects of miR-26a on Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells by a Mesoporous Silica Nanoparticle - PEI - Peptide System</p>

Yan¹,

Lü²,

Zhu³

et al. 2020

IJN

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Introduction: RNA-based therapy for bone repair and regeneration is a highly safe and effective approach, which has been extensively investigated in recent years. However, the molecular stability of RNA agents still remains insufficient for clinical application. High porosity, tunable size, and ideal biodegradability and biosafety are a few of the characters of mesoporous silicon nanoparticles (MSNs) that render them a promising biomaterial carrier for RNA treatment. Materials and Methods: In this study, a novel miR-26a delivery system was constructed based on MSNs. Next, we assessed the miRNA protection of the delivery vehicles. Then, rat bone marrow mesenchymal stem cells (rBMSCs) were incubated with the vectors, and the transfection efficiency, cellular uptake, and effects on cell viability and osteogenic differentiation were evaluated. Results: The results demonstrated that the vectors protected miR-26a from degradation in vitro and delivered it into the cytoplasm. A relatively low concentration of the delivery systems significantly increased osteogenic differentiation of rBMSCs. Conclusion: The vectors constructed in our study provide new methods and strategies for the delivery of microRNAs in bone tissue engineering.

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“…Polylactic acid (PLA)-based scaffolds have been extensively used for bone regeneration [1][2][3][4][5][6], as they have a suitable degradation rate and mechanical properties to replace the tissue in non-load-bearing applications. However, PLA is a hydrophobic material and, as a consequence, its interaction with the extracellular matrix (ECM) is low [7].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Aloe Vera Coated Polylactic Acid Scaffolds for Bone Tissue Engineering

et al. 2020

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3D-printed polylactic acid (PLA) scaffolds have been demonstrated as being a promising tool for the development of tissue-engineered replacements of bone. However, this material lacks a suitable surface chemistry to efficiently interact with extracellular proteins and, consequently, to integrate into the surrounding tissue when implanted in vivo. In this study, aloe vera coatings have been proposed as a strategy to improve the bioaffinity of this type of structures. Aloe vera coatings were applied at three different values of pH (3, 4 and 5), after treating the surface of the PLA scaffolds with oxygen plasma. The surface modification of the material has been assessed through X-ray photoelectron spectroscopy (XPS) analysis and water contact angle measurements. In addition, the evaluation of the enzymatic degradation of the structures showed that the pH of the aloe vera extracts used as coating influences the degradation rate of the PLA-based scaffolds. Finally, the cell metabolic activity of an in vitro culture of human fetal osteoblastic cells on the samples revealed an improvement of this parameter on aloe vera coated samples, especially for those treated at pH 3. Hence, these structures showed potential for being applied for bone tissue regeneration.

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Engineering a multifunctional 3D-printed PLA-collagen-minocycline-nanoHydroxyapatite scaffold with combined antimicrobial and osteogenic effects for bone regeneration

Cited by 140 publications

References 57 publications

Biologically Inspired Collagen/Apatite Composite Biomaterials for Potential Use in Bone Tissue Regeneration—A Review

Biologically Inspired Collagen/Apatite Composite Biomaterials for Potential Use in Bone Tissue Regeneration—A Review

<p>Effects of miR-26a on Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells by a Mesoporous Silica Nanoparticle - PEI - Peptide System</p>

Evaluation of Aloe Vera Coated Polylactic Acid Scaffolds for Bone Tissue Engineering

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