Engineering a Model Cell for Rational Tuning of GPCR Signaling

Shaw, W. M.; Yamauchi, Hitoshi; Mead, Jack; Gowers, Glen-Oliver F.; Bell, David; Öling, David; Larsson, Niklas; Wigglesworth, Mark; Ladds, Graham; Ellis, Tom

doi:10.1016/j.cell.2019.02.023

Cited by 172 publications

(251 citation statements)

References 117 publications

(176 reference statements)

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“…Thus, by using a reporter gene at the end of the pheromone response pathway, a heterologous yeast strain was obtained that acted as a melatonin sensor. By employing the green fluorescent protein as a reporter, the authors detected melatonin production by yeasts using a microplate reader without having to process the sample, but directly from the supernatant (40). The second method was set up to detect melatonin in fermented beverages, and it involved mammalian melatonin receptor MTNR1B and cell lines as a sensor.…”

Section: Development and Optimization Of Analytical Techniques To Detmentioning

confidence: 99%

Melatonin in yeast and fermented beverages: analytical tools for detection, physiological role and biosynthesis

2020

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The recently established relation between the metabolism of aromatic amino acids of yeast and the production of different bioactive molecules during fermentation opens up new and interesting research topics. Among these molecules, melatonin has drawn researchers’ attention in the last decade given its potential benefits for human health. This review summarizes melatonin production in fermented beverages, and conventional and current methods for detecting melatonin in yeast-derived samples. In addition, the role of melatonin in yeast is discussed and the biosynthetic pathway of melatonin is presented in Saccharomyces cerevisiae.

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Section: Development and Optimization Of Analytical Techniques To Detmentioning

confidence: 99%

Melatonin in yeast and fermented beverages: analytical tools for detection, physiological role and biosynthesis

2020

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“…way components(Ostrov et al, 2017;Shaw et al, 2019) may not function as well in strains with altered sterol content.Importantly, recent studies have utilised the yeast pheromone response to separate yeast growth from product generation. The delay of production until cells have grown to a particular cell density (reviewed inVenayak, Anesiadis, Cluett, & Mahadevan, 2015) is advantageous if transgene expression and/or the heterologous product is toxic or if production severely starves the cells of metabolites required for growth Yu et al (2017).…”

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confidence: 99%

The wide‐ranging phenotypes of ergosterol biosynthesis mutants, and implications for microbial cell factories

Johnston

Moses

Rosser

2020

Yeast

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Yeast strains have been used extensively as robust microbial cell factories for the production of bulk and fine chemicals, including biofuels (bioethanol), complex pharmaceuticals (antimalarial drug artemisinin and opioid pain killers), flavours, and fragrances (vanillin, nootkatone, and resveratrol). In many cases, it is of benefit to suppress or modify ergosterol biosynthesis during strain engineering, for example, to increase thermotolerance or to increase metabolic flux through an alternate pathway.However, the impact of modifying ergosterol biosynthesis on engineered strains is discussed sparsely in literature, and little attention has been paid to the implications of these modifications on the general health and well-being of yeast. Importantly, yeast with modified sterol content exhibit a wide range of phenotypes, including altered organization and dynamics of plasma membrane, altered susceptibility to chemical treatment, increased tolerance to high temperatures, and reduced tolerance to other stresses such as high ethanol, salt, and solute concentrations. Here, we review the wide-ranging phenotypes of viable Saccharomyces cerevisiae strains with altered sterol content and discuss the implications of these for yeast as microbial cell factories.

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“…The approach of Ostrov and colleagues using GPCRs is equally versatile and can be adapted to detect target molecules that we have not yet thought of [33]. Similarly, Shaw and colleagues engineered a modified S. cerevisiae cell as a platform for biosensors construction by rational tuning of GPCR signaling [92,93].…”

Section: Technological Developmentsmentioning

confidence: 99%

Yeast-Based Biosensors: Current Applications and New Developments

Martin-Yken

2020

Biosensors

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Biosensors are regarded as a powerful tool to detect and monitor environmental contaminants, toxins, and, more generally, organic or chemical markers of potential threats to human health. They are basically composed of a sensor part made up of either live cells or biological active molecules coupled to a transducer/reporter technological element. Whole-cells biosensors may be based on animal tissues, bacteria, or eukaryotic microorganisms such as yeasts and microalgae. Although very resistant to adverse environmental conditions, yeasts can sense and respond to a wide variety of stimuli. As eukaryotes, they also constitute excellent cellular models to detect chemicals and organic contaminants that are harmful to animals. For these reasons, combined with their ease of culture and genetic modification, yeasts have been commonly used as biological elements of biosensors since the 1970s. This review aims first at giving a survey on the different types of yeast-based biosensors developed for the environmental and medical domains. We then present the technological developments currently undertaken by academic and corporate scientists to further drive yeasts biosensors into a new era where the biological element is optimized in a tailor-made fashion by in silico design and where the output signals can be recorded or followed on a smartphone.

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Engineering a Model Cell for Rational Tuning of GPCR Signaling

Cited by 172 publications

References 117 publications

Melatonin in yeast and fermented beverages: analytical tools for detection, physiological role and biosynthesis

Melatonin in yeast and fermented beverages: analytical tools for detection, physiological role and biosynthesis

The wide‐ranging phenotypes of ergosterol biosynthesis mutants, and implications for microbial cell factories

Yeast-Based Biosensors: Current Applications and New Developments

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