Engineering a material for biomedical applications with electric field assisted processing

Ahmad, Zeeshan; Nangrejo, M.; Edirisinghe, Mohan; Stride, Eleanor; Colombo, Paolo; Zhang, Hongti

doi:10.1007/s00339-009-5359-z

Cited by 36 publications

(20 citation statements)

References 26 publications

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“…Buchko et al fabricated microarray using electrospray deposition with a conductive mask where deposition occurs on both mask and substrate evenly, resulting in the loss of raw materials and poor spot density due to lack of focusing effect. [20][21][22][23] Although Morozov et al used a dielectric mask for electrospray deposition, the deposition efficiency was improved comparing to the method by Buchko et al, the interspot distance was still large (1 mm) and it was limited to the dielectric mask and conductive substrate. 24,25 Kim et al used a dielectric mask and a conductive substrate with a combination of a surface acoustic wave atomizer and electrostatic deposition to fabricate protein chips.…”

Section: Introductionmentioning

confidence: 91%

“…38,39 Most recently, Li et al and Ahmad et al examined inorganic hydroxylapatite nanocrystal pattern formation using template-assisted electrohydrodynamic atomization spraying and their potential biomedical applications. [21][22][23] In contrast, electrospray deposition has not yet been commonly used to fabricate polymeric particle pattern. In our previous studies, controllable size and morphology of biodegradable polymeric particles were achieved by electrospray approach.…”

Section: Introductionmentioning

confidence: 91%

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Electric field controlled electrospray deposition for precise particle pattern and cell pattern formation

et al. 2010

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in Wiley Online Library (wileyonlinelibrary.com).Photolithography, soft lithography, and ink jetting have been used for automated micropattern fabrication. However, most of the methods for microfabrication of surface pattern are limited to the investigation of material properties of substrates with high-cost and complex procedures. In the present study, we show a simple (single-step) yet versatile and robust approach to generate biodegradable polymeric particle patterns on a substrate using electrospray deposition through a mask. Various particle patterns including patterned dots, circles, squares, and bands can be easily formed and the features of particle patterns could also be tailored using different masks and electrostatic focusing effects. Furthermore, cell patterns can be achieved on the surface of particle patterns by blocking the areas without particle deposition on the substrate and culturing cells on the substrate. Polymeric particle patterns and cell patterns developed in this study could be used in the high throughput screening of sustained release formulations, cell-based sensing, and drug discovery. In addition to experimental results, an analysis of the associated electric field is used to investigate quantitatively the nature of focusing effect. Scaling analysis is also applied to obtain the dominate terms in electrospray deposition process.

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Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 91%

Electric field controlled electrospray deposition for precise particle pattern and cell pattern formation

et al. 2010

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“…Favourable physical characteristics of low density combined with high surface area make polymeric nanospheres an ideal drug carrier system (Wang et al, 2007). There are several techniques including emulsion polymerization, solvent evaporation and electrohydrodynamic atomization to prepare nanospheres (Ahmad et al, 2009a;Gunduz et al, 2013;Saito et al, 2006). The flow focusing methods such as T-junction and V-junction microfluidic (VJM) devices have also gained considerable attention (De Koker et al, 2012;Serra and Chang, 2008).…”

Section: Introductionmentioning

confidence: 99%

Utilization of microfluidic V-junction device to prepare surface itraconazole adsorbed nanospheres

Küçük¹,

Ahmad²,

Edirisinghe³

et al. 2014

International Journal of Pharmaceutics

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Itraconazole is widely used as an anti-fungal drug to treat infections. However, its poor aqueous solubility results in low bioavailability. The aim of the present study was to improve the drug release profile by preparing surface itraconazole adsorbed polymethylsilsesquioxane (PMSQ) nanospheres using a V-junction microfluidic (VJM) device. In order to generate nanospheres with rough surface, the process flow rate of perfluorohexane (PFH) was set between 50 and 300 μl min(-1) while the flow rate of PMSQ and itraconazole solution were constant at 300 μl min(-1). Variations in the PFH flow rate enable the controlled size generation of nanospheres. PMSQ nanospheres adsorbing itraconazole were characterized by SEM, FTIR and Zetasizer. The release of itraconazole from PMSQ nanosphere surface was measured using UV spectroscopy. Nanosphere formulations with a range of sphere size (120, 320 and 800 nm diameter) were generated and drug release was studied. 120 nm itraconazole coated PMSQ nanospheres were found to present highest drug encapsulation efficiency and 13% drug loading in a more reproducible manner compared to 320 nm and 800 nm sized nanosphere formulations. Moreover, 120 nm itraconazole coated PMSQ nanospheres (encapsulation efficiency: 88%) showed higher encapsulation efficiency compared to 320 nm (encapsulation efficiency: 74%) and 800 nm (encapsulation efficiency: 62%) sized nanosphere formulations. The itraconazole coated PMSQ nanospheres were prepared continuously at the rate of 2.6 × 10(6) per minute via VJM device. Overall the VJM device enabled the preparation of monodisperse surface itraconazole adsorbed nanospheres with controlled in vitro drug release profile.

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“…These particles were nearly spherical with a diameter of 3 mm and have interconnected porosity with diameters close to 1.3 mm. Ahmad et al (2009) also reported the formation of ceramic bubbles from preceramic polymers via electrospraying.…”

Section: Introductionmentioning

confidence: 99%

Ceramic microparticles and capsules via microfluidic processing of a preceramic polymer

Chen

Colombo

et al. 2010

J. R. Soc. Interface.

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We have developed a robust technique to fabricate monodispersed solid and porous ceramic particles and capsules from single and double emulsion drops composed of silsesquioxane preceramic polymer. A microcapillary microfluidic device was used to generate the monodispersed drops. In this device, two round capillaries are aligned facing each other inside a square capillary. Three fluids are needed to generate the double emulsions. The inner fluid, which flows through the input capillary, and the middle fluid, which flows through the void space between the square and inner fluid capillaries, form a coaxial co-flow in a direction that is opposite to the flow of the outer fluid. As the three fluids are forced through the exit capillary, the inner and middle fluids break into monodispersed double emulsion drops in a single-step process, at rates of up to 2000 drops s 21. Once the drops are generated, the silsesquioxane is cross-linked in solution and the cross-linked particles are dried and pyrolysed in an inert atmosphere to form oxycarbide glass particles. Particles with diameters ranging from 30 to 180 mm, shell thicknesses ranging from 10 to 50 mm and shell pore diameters ranging from 1 to 10 mm were easily prepared by changing fluid flow rates, device dimensions and fluid composition. The produced particles and capsules can be used in their polymeric state or pyrolysed to ceramic. This technique can be extended to other preceramic polymers and can be used to generate unique core -shell multimaterial particles.

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Engineering a material for biomedical applications with electric field assisted processing

Cited by 36 publications

References 26 publications

Electric field controlled electrospray deposition for precise particle pattern and cell pattern formation

Electric field controlled electrospray deposition for precise particle pattern and cell pattern formation

Utilization of microfluidic V-junction device to prepare surface itraconazole adsorbed nanospheres

Ceramic microparticles and capsules via microfluidic processing of a preceramic polymer

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