Engineering a highly selective probe for ratiometric imaging of H₂S_n and revealing its signaling pathway in fatty liver disease

Abstract

<p>Hydrogen polysulfides (H2Sn, n &gt; 1) have continuously been proved to act as impotant signal mediators in many physiological processes. However, the physiological role of H2Sn and their signaling pathway...</p>

Biomarker‐Responsive Fluorescent Probes for In‐Vivo Imaging of Liver Injury

“…To date, increasing reports have underlined the association between anomalous H 2 S level and liver diseases [38] . An appealing strategy was reported for the design of an H 2 S n ‐responsive nanoprobe by Zhang et al, which composed of three steps: screening of the recognition moiety to response H 2 S n , combining two fluorophores with through‐bond energy transfer effect to construct a ratiometric sensor, and the loading of the sensor in self‐assembled amphiphilic copolymer to from the nanoprobe with advanced performances (Figure 8A) [39] . The nanoprobe PPG−Np‐RhPhCO had been successfully applied to observe the production of endogenous and exogenous H 2 S n in cells, zebrafish and the liver tissues of NAFLD mice.…”

“…[45] The fluorescence signal of HeLa cells pre-incubated with probe 1 was distinctly attenuated after the incubation with a typical thiol-depleting agent N-methylmaleimide (NMM), [46] and recovered after the re-addition of thiols. Since GSH, Cys and Hcy share similar molecular structure and biological activities, the discriminative sensing of their co- [39] Copyright 2020, The Royal Society of Chemistry. (B) Schematic illustration for the synthesis steps of 1-PEI-DCNPs.…”

“…[38] An appealing strategy was reported for the design of an H 2 S nresponsive nanoprobe by Zhang et al, which composed of three steps: screening of the recognition moiety to response H 2 S n , combining two fluorophores with through-bond energy transfer effect to construct a ratiometric sensor, and the loading of the sensor in self-assembled amphiphilic copolymer to from the nanoprobe with advanced performances (Figure 8A). [39] The nanoprobe PPGÀ Np-RhPhCO had been successfully applied to observe the production of endogenous and exogenous H 2 S n in cells, zebrafish and the liver tissues of NAFLD mice. Moreover, the probe helped to elucidate the relationship between ROS and H 2 S n , a stronger signal was exhibited in the mice stimulated The structure of S-BODIPY and the mechanism of response to HClO.…”

“… (A) Design strategy of PPG−Np‐RhPhCO for H 2 S n . Reproduced with permission [39] . Copyright 2020, The Royal Society of Chemistry.…”

Highly Selective Fluorescent Probe Design for Visualizing Hepatic Hydrogen Sulfide in the Pathological Progression of Nonalcoholic Fatty Liver

“…19,20 Recent studies have found that the H 2 S biosynthetic pathways (including CBS/H 2 S, CSE/H 2 S, MPST/H 2 S) in the liver are closely related to the regulation of fatty acids (FFA) and the treatment of NAFLD. 21−23 Meanwhile, our laboratory's early research 24 at the cellular level found that endogenous H 2 S in NAFLD increases with the aggravation of oxidative stress, maintaining the intracellular oxidative balance. However, due to the complexity and difficulty of in vivo research, the levels of hepatic endogenous H 2 S in the occurrence and development of NAFLD, and its specific mechanism of action, are still unclear.…”