Engineering a FRET strategy to achieve a ratiometric two-photon fluorescence response with a large emission shift and its application to fluorescence imaging

Abstract

A FRET strategy was applied to develop a ratiometric two-photon fluorescent probe with a large emission shift for imaging in cells and tissues.

Dual‐Reactable Fluorescent Probes for Highly Selective and Sensitive Detection of Biological H2S

“…Such a fluorescent turn‐on response could be clearly visualized by the naked eye (Figure a). To our knowledge, the observed increase in fluorescence emission for 2 is the largest among the fluorescent probes reported so far for H 2 S …”

“…[6] Fluorescence-based methodsh ave recently emerged as au seful approach forH 2 Sd etection in biological systems. [7][8][9][10][11][12][13][14][15][16][17] An ideal intracellular H 2 Ss ensor should have al arge dynamic range for the selective detection of H 2 So ver higheri ntracellular concentrations of biothiols (such as millimolar concentrations of reduced glutathione,G SH). The reactivity of the probe toward H 2 Si sa lso essential forr eal-time detection.…”

“…Such af luorescent turn-on response could be clearly visualized by the naked eye ( Figure 3a). To our knowledge, the observed increase in fluorescencee mission for 2 is the largest among the fluorescent probes reported so far for H 2 S. [7][8][9][10][11][12][13][14][15][16][17] To determinet he detection limit for 1 and 2,t he fluorescence intensity change was closely monitored by addition of variousc oncentrationso fH 2 St oasolution of the probe (Figure 3b and Figure S1). For both probes, the fluorescencei ntensity wasl inearly related to the concentration of H 2 Sf rom 0t o 100 mm,i ndicating al arge dynamic range for the detection of H 2 S. The detection limit for 1 or 2 was calculated to be 27 and 24 nm,r espectively,b yu sing the 3s/k method.…”

Photostability Enhancement of Fluorenone-Based Two-Photon Fluorescent Probes for Cellular Nucleus Monitoring and Imaging

“…[5][6][7] In addition, two-photon transtion has a strong selective excitation, which is conducive to the high-resolution imaging of some special sites in biological tissues. [8][9][10] Therefore, with the continuous development of the two-photon confocal fluorescence microscope-which is considered to have excellent performance because of its three-dimensional spatial selectivity, high penetration depth, low Rayleigh scattering, and femtosecond laser source operating-two-photon excitation fluorescent (TPEF) probes have gradually gained attention in scientific research. 11,12 Compared with one-photon fluorescent probes, two-photon fluorescent probes can label deep sites in biogical samples by virtue of three dimensional imaging capabilities of two-photon absorption.…”

A fast‐responsive fluorescent probe for sulfite and its bioimaging

“…However, these methods usually require tedious sample and reagent preparation or complicated instruments that are not suitable for routine analysis. As an alternative, the fluorescence method has received much attention due its the advantages of convenience, high sensitivity, fast response and ease of live-cell imaging (14)(15)(16). Fluorescent probes for SO 3 2-have been mainly based on the addition to an aldehyde moiety or a nucleophilic addition (17)(18)(19)(20)(21)(22)(23)(24)(25), and levulinate cleavage mediated by SO 3 2- (26)(27)(28)(29)(30)(31)(32)(33)(34)(35).…”