Engineering 3D-Bioplotted scaffolds to induce aligned extracellular matrix deposition for musculoskeletal soft tissue replacement

Warren, Paul B.; Huebner, Pedro; Spang, Jeffrey T.; Shirwaiker, Rohan A.; Fisher, Matthew B.

doi:10.1080/03008207.2016.1276177

Cited by 27 publications

(28 citation statements)

References 51 publications

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“…[1] The capability of the scaffold to be infiltrated by cells and to support cell proliferation and ECM formation is essential whether the cells are seeded in vitro in classical bioreactor-based strategies [22,23] or attracted to the scaffold in vivo once implanted, as envisioned by in situ tissue engineering approaches. [24][25][26][27][28][29] Matching porous properties is therefore a cornerstone in the development of scaffolds for tissue engineering Among the several methods that have been reported to create porosity, [1] salt-leaching coupled with gas foaming (SL/GF) technique is considered as one of the best approaches since it offers the possibility not only to tune the pore-size but, importantly, to obtain an interconnected porous network. [30] This technique is based on the dispersion of a porogen (e.g.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Macroporous click-elastin-like hydrogels for tissue engineering applications

Fernández‐Colino

Wolf

Keijdener

et al. 2018

Materials Science and Engineering: C

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Elastin is a key extracellular matrix (ECM) protein that imparts functional elasticity to tissues and therefore an attractive candidate for bioengineering materials. Genetically engineered elastin-like recombinamers (ELRs) maintain inherent properties of the natural elastin (e.g. elastic behavior, bioactivity, low thrombogenicity, inverse temperature transition) while featuring precisely controlled composition, the possibility for biofunctionalization and non-animal origin. Recently the chemical modification of ELRs to enable their crosslinking via a catalyst-free click chemistry reaction, has further widened their applicability for tissue engineering. Despite these outstanding properties, the generation of macroporous click-ELR scaffolds with controlled, interconnected porosity has remained elusive so far. This significantly limits the potential of these materials as the porosity has a crucial role on cell infiltration, proliferation and ECM formation. In this study we propose a strategy to overcome this issue by adapting the salt leaching/gas foaming technique to click-ELRs. As result, macroporous hydrogels with tuned pore size and mechanical properties in the range of many native tissues were reproducibly obtained as demonstrated by rheological measurements and quantitative analysis of fluorescence, scanning electron and two-photon microscopy images. Additionally, the appropriate size and interconnectivity of the pores enabled smooth muscle cells to migrate into the click-ELR scaffolds and deposit extracellular matrix. The macroporous structure together with the elastic performance and bioactive character of ELRs, the specificity and non-toxic character of the catalyst-free click-chemistry reaction, make these scaffolds promising candidates for applications in tissue regeneration. This work expands the potential use of ELRs and click chemistry systems in general in different biomedical fields.

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Section: Accepted Manuscriptmentioning

confidence: 99%

Macroporous click-elastin-like hydrogels for tissue engineering applications

Fernández‐Colino

Wolf

Keijdener

et al. 2018

Materials Science and Engineering: C

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“…Additionally, anisotropic scaffolds result in improved cellular and extracellular matrix (ECM) alignment, increased ECM deposition, and an increased rate of mechanical improvement . Groups have attempted to recreate the anisotropy of the native meniscus utilizing fabrication techniques such as weaving, electrospinning, or 3D‐printing . However, these devices have never successfully matched the mechanics of the native meniscus in both compression and tension, and fewer have demonstrated the ability to reduce contact stresses on the cartilage under compressive loading …”

Section: Introductionmentioning

confidence: 99%

Biomechanical characterization of a novel collagen‐hyaluronan infused 3D‐printed polymeric device for partial meniscus replacement

et al. 2019

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The menisci transmit load by increasing the contact area and decreasing peak contact stresses on the articular surfaces. Meniscal lesions are among the most common orthopedic injuries, and resulting meniscectomies are associated with adverse polycaprolactone contact mechanics changes and, ultimately, an increased likelihood of osteoarthritis. Meniscus scaffolds were fabricated by 3D‐printing a network of circumferential and radial filaments of resorbable polymer (poly(desaminotyrosyl‐tyrosine dodecyl ester dodecanoate)) and infused with collagen‐hyaluronan. The scaffold demonstrated an instantaneous compressive modulus (1.66 ± 0.44 MPa) comparable to native meniscus (1.52 ± 0.59 MPa). The scaffold aggregate modulus (1.33 ± 0.51 MPa) was within 2% of the native value (1.31 ± 0.36 MPa). In tension, the scaffold displayed a comparable stiffness to native tissue (127.6–97.1 N/mm) and an ultimate load of 33% of the native value. Suture pull‐out load of scaffolds (83.1 ± 10.0 N) was within 10% of native values (91.5 ± 15.4 N). Contact stress analysis demonstrated the scaffold reduced peak contact stress by 60–67% and increased contact area by 38%, relative to partial meniscectomy. This is the first meniscal scaffold to match both the axial compressive properties and the circumferential tensile stiffness of the native meniscus. The improvement of joint contact mechanics, relative to partial meniscectomy alone, motivates further investigation using a large animal model. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2457–2465, 2019.

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“…These findings suggest that co-culture of MSCs and fibrochondrocytes in tissue-engineered constructs may strike a balance between the synthetic phenotype of the MSCs and matrix remodeling phenotype of the fibrochondrocytes. In addition to this cell-mediated control of ECM organization, Warren et al show that interstrand spacing in 3D-bioplotted scaffolds can control collagen alignment (12). In particular, as scaffold interstrand spacing decreases, collagen alignment increases.…”

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confidence: 99%