Engineered Tobacco mosaic virus mutants with distinct physical characteristics in planta and enhanced metallization properties

Kadri, Anan; Maiß, E.; Amsharov, Nadja; Bittner, Alexander M.; Balci, Sinan; Kern, Klaus; Jeske, Holger; Wege, Christina

doi:10.1016/j.virusres.2011.01.014

Cited by 70 publications

(59 citation statements)

References 68 publications

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“…[36,78] In addition to charged amino acids, the polypeptides or oligopeptides with high binding capability to inorganic minerals play an essential role in biomineralization processes (Figure 4). [81,82] Moreover, the M13 phage engineered with a glutamate trimer or tetramer on the major p8 capsid protein can increase the electrostatic interaction with cationic metal ions or metal precursors, which benefits the spontaneous formation of a polycrystalline or metal oxide surrounded virus coat ( Figure 4). [81,82] Moreover, the M13 phage engineered with a glutamate trimer or tetramer on the major p8 capsid protein can increase the electrostatic interaction with cationic metal ions or metal precursors, which benefits the spontaneous formation of a polycrystalline or metal oxide surrounded virus coat ( Figure 4).…”

Section: Genetic Engineeringmentioning

confidence: 99%

Therapeutic Potential of Biomineralization‐Based Engineering

Wang

Xiao

Hao

et al. 2018

Advanced Therapeutics

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In nature, the biomineralization processes of living organisms produce a wide range of organic-inorganic hybrid materials to achieve various functions. In particular, egg shells can provide extra protection for embryos and maintain air exchange. Inspired by such phenomena, it is assumed that the engineering of organisms with biomimetic materials can lead to significant improvement in organism function. This review summarizes recent progress in biomineralization-based techniques for organism engineering, and demonstrates the therapeutic potential enabled by these techniques. The design and synthesis approaches of biomineralization-based engineering are systemically introduced to guide the controlled modification of different organisms including viruses, bacteria, cells, and proteins using in situ biomineralization, bottom-up self-assembly, chemical and genetic engineering. Tailored organisms promise delivery, protection, cell therapy, vaccine improvement as well as therapeutic detection and imaging. The present review aims to propose a biomineralization-based strategy to promote functional evolution of these organisms, which promises to meet the increasing demand for new therapeutic purpose.

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Section: Genetic Engineeringmentioning

confidence: 99%

Therapeutic Potential of Biomineralization‐Based Engineering

Wang

Xiao

Hao

et al. 2018

Advanced Therapeutics

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“…pTMV-C (also named p843peTMVΔ9353-4411), a 4941 bp plasmid containing a truncated cDNA copy of TMV U1 genomic RNA corresponding to TMV RNA residues 4412-6395, was prepared by restriction hydrolysis of p843pe35TMVr1 85 with SnaBI and AatII followed by religation. pTMV-C served as template for polymerase chain reactions (PCR) amplifying cDNA portions containing the OAs, using Taq DNA polymerase (Qiagen, Hilden, Germany) and two pairs of primers ( The resulting PCR products (1893 or 2138 base pairs [bps]) were cut with XhoI, and, after purification, ligated with each other using T4 DNA ligase.…”

Section: Cloning and In Vitro Transcriptionmentioning

confidence: 99%

RNA-controlled assembly of tobacco mosaic virus-derived complex structures: from nanoboomerangs to tetrapods

et al. 2015

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The in vitro assembly of artificial nanotubular nucleoprotein shapes based on tobacco mosaic virus-(TMV-)-derived building blocks yielded different spatial organizations of viral coat protein subunits on genetically engineered RNA molecules, containing two or multiple TMV origins of assembly (OAs). The growth of kinked nanoboomerangs as well as of branched multipods was determined by the encapsidated RNAs. A largely simultaneous initiation at two origins and subsequent bidirectional tube elongation could be visualized by transmission electron microscopy of intermediates and final products. Collision of the nascent tubes' ends produced angular particles with well-defined arm lengths. RNAs with three to five OAs generated branched multipods with a maximum of four arms. The potential of such an RNA-directed self-assembly of uncommon nanotubular architectures for the fabrication of complex multivalent nanotemplates used in functional hybrid materials is discussed.

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“…Aiming at intimate connections between biological and inorganic technical or implant materials, spatially selective mineral deposition has been investigated for differently shaped plant viruses including the filamentous Potato virus X [28], the spherical Cowpea mosaic virus [29,30,31,32] and the nanotubular Tobacco mosaic virus (TMV, [33,34,35,36,37]). TMV has emerged as an especially suitable scaffold in the field of bio-inspired mineralization, due to the dimensions and homogeneity of the viral particles, nanometrically precise arrangements of chemical groups, sustainable multiplication protocols, and an amenability to hierarchical combination with other material classes.…”

Section: Resultsmentioning

confidence: 99%

Biogenic and Synthetic Peptides with Oppositely Charged Amino Acids as Binding Sites for Mineralization

et al. 2017

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Proteins regulate diverse biological processes by the specific interaction with, e.g., nucleic acids, proteins and inorganic molecules. The generation of inorganic hybrid materials, such as shell formation in mollusks, is a protein-controlled mineralization process. Moreover, inorganic-binding peptides are attractive for the bioinspired mineralization of non-natural inorganic functional materials for technical applications. However, it is still challenging to identify mineral-binding peptide motifs from biological systems as well as for technical systems. Here, three complementary approaches were combined to analyze protein motifs consisting of alternating positively and negatively charged amino acids: (i) the screening of natural biomineralization proteins; (ii) the selection of inorganic-binding peptides derived from phage display; and (iii) the mineralization of tobacco mosaic virus (TMV)-based templates. A respective peptide motif displayed on the TMV surface had a major impact on the SiO2 mineralization. In addition, similar motifs were found in zinc oxide- and zirconia-binding peptides indicating a general binding feature. The comparative analysis presented here raises new questions regarding whether or not there is a common design principle based on acidic and basic amino acids for peptides interacting with minerals.

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Engineered Tobacco mosaic virus mutants with distinct physical characteristics in planta and enhanced metallization properties

Cited by 70 publications

References 68 publications

Therapeutic Potential of Biomineralization‐Based Engineering

Therapeutic Potential of Biomineralization‐Based Engineering

RNA-controlled assembly of tobacco mosaic virus-derived complex structures: from nanoboomerangs to tetrapods

Biogenic and Synthetic Peptides with Oppositely Charged Amino Acids as Binding Sites for Mineralization

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