Engineered spider silk-based 2D and 3D materials prevent microbial infestation

Kumari, Sushma; DeSimone, Elise; Spengler, Christian; Trossmann, Vanessa T.; Lücker, Susanne; Hudel, Martina; Jacobs, Karin; Krämer, Norbert; Scheibel, Thomas

doi:10.1016/j.mattod.2020.06.009

Cited by 33 publications

(40 citation statements)

References 71 publications

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“…In this previous study, S. aureus was also tested and showed a low adhesion force to the spider silk surfaces, which explains the resulting low colony numbers. [44] Next, uncoated and coated silicone surfaces incubated with B. diminuta, R. pickettii, S. aureus and P. acnes (Fig. 3) were analysed using SEM, which confirmed that regardless of the silicone surface, the spider silk coating substantially restricted the attachment, growth and microbial colonisation.…”

Section: Bacterial Repellence Of Spider Silk-coated Silicone Surfacesmentioning

confidence: 66%

“…[39,40] Recently published results indicate that bacterial infestation of spider silk surfaces is inhibited by microberepellent properties of the material´s surface rather than by antibacterial means. [41][42][43][44] As eADF4(C16) lacks cell binding motifs, like most so far identified spider silk proteins, eADF4(C16)-coated implants and catheters display significantly reduced adhesion and proliferation of any cells as compared to non-treated ones. [31,45] When transplanted into rats in vivo, eADF4(C16)-coated silicone implants exhibited a substantial reduction in capsular contracture.…”

Section: Introductionmentioning

confidence: 99%

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Microbial repellence properties of engineered spider silk coatings prevent biofilm formation of opportunistic bacterial strains

et al. 2021

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Bacterial infections are well recognised to be one of the most important current public health problems. Inhibiting adhesion of microbes on biomaterials is one approach for preventing inflammation. Coatings made of recombinant spider silk proteins based on the consensus sequence of Araneus diadematus dragline silk fibroin 4 have previously shown microbe-repellent properties. Concerning silicone implants, it has been further shown that spider silk coatings are effective in lowering the risk of capsular fibrosis. Here, microbial repellence tests using four opportunistic infection-related strains revealed additional insights into the microbe-repellent properties of spider silk-coated implants, exemplarily shown for silicone surfaces. Graphic Abstract

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Section: Bacterial Repellence Of Spider Silk-coated Silicone Surfacesmentioning

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Microbial repellence properties of engineered spider silk coatings prevent biofilm formation of opportunistic bacterial strains

et al. 2021

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“…Both proteins were produced and purified as reported earlier. [6,17,18] Spider silk proteins were dissolved in 6 m guanidinium thiocyanate (Carl Roth, Karlsruhe, Germany) and dialysed against 10 mm Tris buffer, pH 7.5 for several hours, whereas the dialysis of eADF4(W16) was done at 4 8C. Concentration adjustment was conducted, if necessary, by follow-up dialysis in 20 % (w/v) poly(ethylene glycol) (PEG, 20 kDa, Carl Roth, Karlsruhe, Germany) as reported previously [7a] or using a high vacuum concentrator (Speedvac, Eppendorf).…”

Section: Methodsmentioning

confidence: 99%

“…Potassium phosphate induced fibrillisation of the negatively charged eADF4(C16), [6] which was used as positive control, and its mechanism of fibrillisation has been reported previously. [11] The positively charged eADF4(k16) [17] showed accelerated protein aggregation and phase separation in the presence of potassium phosphate, and the uncharged eADF4-(W16) [18] showed particle formation, indicating a shift towards lower critical potassium phosphate concentrations for particle formation for this variant. The impact of DMSO was analysed in aqueous-organic binary mixtures [19] with different volumes.…”

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confidence: 99%

Recombinant Spider Silk Gels Derived from Aqueous–Organic Solvents as Depots for Drugs

et al. 2021

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Hydrogels are widely used in various biomedical applications, as they cannot only serve as materials for biofabrication but also as depots for the administration of drugs. However, the possibilities of formulation of water‐insoluble drugs in hydrogels are rather limited. Herein, we assembled recombinant spider silk gels using a new processing route with aqueous–organic co‐solvents, and the properties of these gels could be controlled by the choice of the co‐solvent. The presence of the organic co‐solvent further enabled the incorporation of hydrophobic drugs as exemplarily shown for 6‐mercaptopurine. The developed gels showed shear‐thinning behaviour and could be easily injected to serve, for example, as drug depots, and they could even be 3D printed to serve as scaffolds for biofabrication. With this new processing route, the formulation of water‐insoluble drugs in spider silk‐based depots is possible, circumventing common pharmaceutical solubility issues.

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“…It is known that the surface chemistry, hydrophobicity and surface charge [31,32] or functional groups deposited on top of a surface influence the bacterial adhesion process. Such functional groups could be for instance proteins, such as silk proteins [33,34], or bodily fluids such as blood plasma [35,36] or salivary macromolecules [37,38,14]. For the purpose of this paper, we therefore use wellcharacterized samples and also examine the influence of conditioning films consisting exclusively of the macromolecules present in either saliva or blood plasma because any material in the body is inevitably in contact with host fluids.…”

Section: Introductionmentioning

confidence: 99%

Hydroxyapatite pellets as versatile model surfaces for systematic studies on enamel

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Faidt

McMillan

et al. 2021

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Research into materials for medical application draws inspiration from naturally occurring or synthesized surfaces, just like many other research directions. For medical application of materials, particular attention has to be paid to biocompatibility, osseointegration and bacterial adhesion behavior. To understand their properties and behavior, experimental studies with natural materials such as teeth are strongly required. The results, however, may be highly case-dependent because natural surfaces have the disadvantage of being subject to wide variations, for instance in their chemical composition, structure, morphology, roughness, and porosity. A synthetic surface which mimics enamel in its performance with respect to bacterial adhesion and biocompatibility would, therefore, facilitate systematic studies much better. In this study, we discuss the possibility of using hydroxyapatite (HAp) pellets to simulate the surfaces of teeth and show the possibility and limitations of using a model surface. We performed single-cell force spectroscopy with single Staphylococcus aureus cells to measure adhesion-related parameters such as adhesion force and rupture length of adhesins binding to HAp and enamel. We also examine the influence of blood plasma and saliva on the adhesion properties of S. aureus. The results of these measurements are matched to water wettability, elemental composition of the samples and the change in the macromolecules adsorbed over time. We found that the adhesion properties of S. aureus were similar on both samples under all conditions: Significant decreases in adhesion strength were found equally in the presence of saliva or blood plasma on both surfaces. We therefore conclude that HAp pellets are a good alternative for natural dental material. This is especially true when slight variations in the physicochemical properties of the natural materials may affect the experimental series.

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Engineered spider silk-based 2D and 3D materials prevent microbial infestation

Cited by 33 publications

References 71 publications

Microbial repellence properties of engineered spider silk coatings prevent biofilm formation of opportunistic bacterial strains

Microbial repellence properties of engineered spider silk coatings prevent biofilm formation of opportunistic bacterial strains

Recombinant Spider Silk Gels Derived from Aqueous–Organic Solvents as Depots for Drugs

Hydroxyapatite pellets as versatile model surfaces for systematic studies on enamel

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