Engineered silica nanocarriers as a high-payload delivery vehicle for antioxidant enzymes

Ambati, Jyothirmai; Lopez, Alexander M.; Cochran, David B.; Wattamwar, Paritosh P.; Bean, Kala; Dziubla, Thomas D.; Rankin, Stephen E.

doi:10.1016/j.actbio.2012.02.012

Cited by 26 publications

(15 citation statements)

References 64 publications

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“…In the past decade, mesoporous silica nanoparticles (MSNs) have shown significant advantages over traditional nanotransporters as in vivo delivery systems for exogenous molecules, due to their tailored mesoporous structure, high surface area, and excellent biocompatibility [20][21][22][23]. While the intracellular uptake of MSNs into mammalian cells has been widely studied, the investigation of their uptake by plant cells remains a relatively dormant field.…”

mentioning

confidence: 99%

Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells

Xia

Dong

Zhang

et al. 2012

Sci. China Life Sci.

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The characteristics of the interactions co-cultures of ultrafine mesoporous silica nanoparticles (MSNs) and the Liriodendron hybrid suspension cells were systematically investigated using laser scanning confocal microscope (LSCM) and scanning electron microscopy (SEM). Using fluorescein isothiocyanate (FITC) labeling, the LSCM observations demonstrated that MSNs (size, 5-15 nm) with attached FITC molecules efficiently penetrated walled plant cells through endocytic pathways, but free FITC could not enter the intact plant cells. The SEM measurements indicated that MSNs readily aggregated on the surface of intact plant cells, and also directly confirmed that MSNs could enter intact plant cells; this was achieved by determining the amount of silicon present. After 24 h of incubation with 1.0 mg mL 1 of MSNs, the viability of the plant cells was analyzed using fluorescein diacetate staining; the results showed that these cells retained high viability, and no cell death was observed. Interestingly, after the incubation with MSNs, the Liriodendron hybrid suspension cells retained the capability for plant regeneration via somatic embryogenesis. Our results indicate that ultrafine MSNs hold considerable potential as nano-carriers of extracellular molecules, and can be used to investigate in vitro gene-delivery in plant cells. Along with developments in the application of nanotechnology from animal science and medical research to plant science research, the impact of engineered nanomaterials on plant systems has attracted increasing attention, the areas including (i) the delivery of fertilizers, herbicides, pesticides and exogenous genes, (ii) the improvement of the growth of plants, and (iii) nanotoxicity research for plant cells [1][2][3][4][5][6][7][8][9][10]. However, compared with mammalian cells, the plant cell wall which is composed of cross-linked polysaccharides (cellulose, hemicelluloses, and pectin) represents an extra barrier surrounding the cell membrane that hinders the passage of nanoparticles into plant cells. To avoid the inhibiting effects of the plant cell wall, free protoplasts (which are prepared via the removal of the cell wall using cellulase treatments) have been used previously to study the internalization of various nanoparticles (e.g., CdSe/ZnS quantum dots (QDs), polystyrene nanospheres, poly(phenylene ethynelene) nanoparticles, and carbon nanomaterials) [11][12][13]. However, protoplast-based transformation methods hold disadvantages in that the viability of the protoplasts and their ability to divide are strongly reduced by the chemicals that are applied to disorganize the cell wall. For this reason, recent studies have focused on intact plant cells; it was found to that they are able to achieve endocytosis to directly internalize single-walled carbon nanotubes (CNTs), CdSe/ZnS QDs, or poly (amidoamine) dendrimer from the

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mentioning

confidence: 99%

Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells

Xia

Dong

Zhang

et al. 2012

Sci. China Life Sci.

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“…2.5 ml of ammonium solution was dispersed into 40 ml of 0.32 M calcium nitrate solution under ultrasound irradiation (Ambati et al 2012, Poinern et al 2009). 60 ml of 0.19 M KH 2 PO 4 solution was added slowly with stirring while, maintaining pH 9 throughout the experiment lead to form white precipitate.…”

Section: Preparation Of Nanoceramic Core (Nc)mentioning

confidence: 99%

Novel catalase loaded nanocores for the treatment of inflammatory bowel diseases

Parihar

Srivastava

Patel

et al. 2016

Artificial Cells, Nanomedicine, and Biotechnology

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Inflammatory bowel disease (IBD) is an inflammatory disorder of the digestive tract reported to be primarily caused by oxidative stress. In this study, alginate encapsulated nanoceramic carriers were designed to deliver acid labile antioxidant enzyme catalase orally. Complete system was characterized for size, loading efficiency, in vitro antioxidant assay and in vitro release. The prepared nanoceramic system was found to be spherical with diameter of 925 ± 6.81 nm. The in vitro release data followed the Higuchi model in acidic buffer whereas in alkaline pH sustained and almost first order release of enzyme was observed up to 6 h.

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“…Intracellular protein delivery holds promise for a range of biomedical applications, [1] such as cancer therapy, [2,3] vaccination, and enzyme based therapeutics. [4] However, therapeutic proteins are susceptible to proteolysis and denaturation, limiting their efficacy in the body.…”

Section: Introductionmentioning

confidence: 99%

“…Also mesoporous silica nanoparticles (MSNs) have been studied as carriers for a variety of biomolecules including anticancer drugs, oligonucleotides, and proteins. [2,[12][13][14][15][16][17][18][19][20][21][22][23][24] However, most MSNs used in drug delivery studies to date typically have pores with diameters up to 4 nm, thereby limiting their use as efficient carriers for high the mesoporous silica nanoparticles (MSNs/cytC). The maximum cytC loading capacity of these cuboidal MSNs was determined to be 470 µg mg −1 MSNs (Figure 2a, black curve).…”

Section: Introductionmentioning

confidence: 99%

Membrane Fusion Mediated Intracellular Delivery of Lipid Bilayer Coated Mesoporous Silica Nanoparticles

Yang

Lamers

et al. 2017

Adv Healthcare Materials

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Protein delivery into the cytosol of cells is a challenging topic in the field of nanomedicine, because cellular uptake and endosomal escape are typically inefficient, hampering clinical applications. In this contribution cuboidal mesoporous silica nanoparticles (MSNs) containing disk‐shaped cavities with a large pore diameter (10 nm) are studied as a protein delivery vehicle using cytochrome‐c (cytC) as a model membrane‐impermeable protein. To ensure colloidal stability, the MSNs are coated with a fusogenic lipid bilayer (LB) and cellular uptake is induced by a complementary pair of coiled‐coil (CC) lipopeptides. Coiled‐coil induced membrane fusion leads to the efficient cytosolic delivery of cytC and triggers apoptosis of cells. Delivery of these LB coated MSNs in the presence of various endocytosis inhibitors strongly suggests that membrane fusion is the dominant mechanism of cellular uptake. This method is potentially a universal way for the efficient delivery of any type of inorganic nanoparticle or protein into cells mediated by CC induced membrane fusion.

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Engineered silica nanocarriers as a high-payload delivery vehicle for antioxidant enzymes

Cited by 26 publications

References 64 publications

Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells

Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells

Novel catalase loaded nanocores for the treatment of inflammatory bowel diseases

Membrane Fusion Mediated Intracellular Delivery of Lipid Bilayer Coated Mesoporous Silica Nanoparticles

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