Engineered Shewanella oneidensis-reduced graphene oxide biohybrid with enhanced biosynthesis and transport of flavins enabled a highest bioelectricity output in microbial fuel cells

Lin, Tao; Ding, Wenqi; Sun, Liming; Wang, Lei; Liu, Chen‐Guang; Song, Hao

doi:10.1016/j.nanoen.2018.05.072

Cited by 96 publications

(62 citation statements)

References 78 publications

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“…[ 17 ] The membrane permeability of S. xiamenensis and S. xiamenensis ‐CDs was measured by the N ‐phenyl‐1‐naphthylamine uptake assay. [ 18 ] As shown in Figure S10, Supporting Information, the S. xiamenensis ‐CDs strain displayed the same fluorescence emission intensity as that of the S. xiamenensis strain at 415 nm, indicating the membrane permeability of S. xiamenensis was unaffected by the internalization of CDs. Thus, we concluded that the high yield of extracellular flavins is due to the internalized CDs that stimulate the intracellular physiology response rather than to membrane penetration.…”

Section: Methodsmentioning

confidence: 94%

Internalized Carbon Dots for Enhanced Extracellular Electron Transfer in the Dark and Light

Liu

et al. 2020

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Cellular internalization of nanomaterials to endow cells with more functionalities is highly desirable. Herein, a straightforward strategy for internalizing red‐emission carbon dots (CDs) into Shewanella xiamenensis is proposed. This suggests that the internalized CDs not only afford enhanced conductivity of bacteria but also trigger the cellular physiological response to secrete abundant electron shuttles to aid the boosting of extracellular electron transfer (EET) efficiency. Additionally, once illuminated, internalized CDs can also serve as light absorbers to allow for photogenerated electrons to be transferred into cellular metabolism to further facilitate light‐enhanced EET processes. Specifically, the findings advance the fundamental understanding of the interaction between internalized carbon‐based semiconductor and cells in the dark and light, and provide a facile and effective strategy for enhancing EET efficiency.

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Section: Methodsmentioning

confidence: 94%

Internalized Carbon Dots for Enhanced Extracellular Electron Transfer in the Dark and Light

Liu

et al. 2020

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“…In the last decade, extensive studies on exoelectrogens were conducted from multidisciplinary fields to elucidate fundamental mechanisms of EET. Many approaches to promote power generation primarily involved facilitating EET between bacteria and electrodes to promote the EET rate 20 , 21 , including broadening and strengthening substrate utilization 60 , optimizing conductive c -type cytochromes systems 61 , promoting electron shuttle biosynthesis and transportation 39 , 40 , 62 , and constructing electroactive biofilms 41 . However, whether the intracellular electrons pool had any effect on the EET rate remained uncharacterized.…”

Section: Discussionmentioning

confidence: 99%

“…Based on these two underlying EET mechanisms, a number of synthetic biology strategies have been developed to enhance the rate of EET in S. oneidensis . For example, a synthetic riboflavin biosynthesis pathway from Bacillus subtilis was incorporated into S. oneidensis , resulting in a significant increase in secreted riboflavin and a subsequently improved EET rate 39 , 40 . Cyclic-di-GMP (a second messenger) was overexpressed in S. oneidensis to promote electroactive biofilm formation and the EET rate 41 .…”

Section: Introductionmentioning

confidence: 99%

Modular engineering to increase intracellular NAD(H/+) promotes rate of extracellular electron transfer of Shewanella oneidensis

Li¹,

Li²,

Cao³

et al. 2018

Nat Commun

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139

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The slow rate of extracellular electron transfer (EET) of electroactive microorganisms remains a primary bottleneck that restricts the practical applications of bioelectrochemical systems. Intracellular NAD(H/+) (i.e., the total level of NADH and NAD+) is a crucial source of the intracellular electron pool from which intracellular electrons are transferred to extracellular electron acceptors via EET pathways. However, how the total level of intracellular NAD(H/+) impacts the EET rate in Shewanella oneidensis has not been established. Here, we use a modular synthetic biology strategy to redirect metabolic flux towards NAD+ biosynthesis via three modules: de novo, salvage, and universal biosynthesis modules in S. oneidensis MR-1. The results demonstrate that an increase in intracellular NAD(H/+) results in the transfer of more electrons from the increased oxidation of the electron donor to the EET pathways of S. oneidensis, thereby enhancing intracellular electron flux and the EET rate.

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“…Nakamura et al reported a 80-fold increase of the current delivered by the electromicrobiological device with Shewanella liohilica when they added iron oxide colloids as electron relay. [12] Finally, redox polymer are also proposed to improve EET in living biocomposite electrodes. [4] Recent try with graphene oxide have allowed to enhance 24fold the anodic current density and 75-fold the density current to the cathode [6] with the formation of a hybrid biofilm resulting from the reduction of graphene oxide by S. oneidensis.…”

Section: Introductionmentioning

confidence: 99%

“…More recently, the system was improved by the combination of biocomposite formation and engineered S. oneidensis. [12] Finally, redox polymer are also proposed to improve EET in living biocomposite electrodes. [5] Those artificial biofilms would greatly benefit to electrochemical bacterial devices such as microbial fuel cell (MFC), where electrons are transferred to the anode and generate energy [13,14] or sustain biosensors.…”

Section: Introductionmentioning

confidence: 99%

Protamine Promotes Direct Electron Transfer Between Shewanella oneidensis Cells and Carbon Nanomaterials in Bacterial Biocomposites

Pinck

Jorand

et al. 2019

ChemElectroChem

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In this work, we studied the direct electron transfer reactions occurring in a biocomposite resulting from the self-assembly of Shewanella oneidensis MR-1 cells with different carbon nanomaterials (multi-walled carbon nanotubes, graphene oxide, Ketjen black or ordered mesoporous carbon) and protamine. The system has been studied with dynamic light scattering, scanning electron microscopy, epifluorescence microscopy and electrochemistry. Although protamine is known to have an antimicrobial activity, the interaction with carbon nanomaterials largely limits this effect. Formate and lactate oxidation and fumarate reduction have been studied. In the presence of 50 mM formate or 50 mM fumarate, the anodic current was lower than the cathodic one with the biocomposite prepared with protamine, while it was found similar with the biocomposite prepared with cytochromes c 1 or c 3 DvH, suggesting different electron transfer pathways. The redox potential observed for fumarate reduction (half-wave potential) was found between À 0.1 V and À 0.2 V vs. SHE with an onset potential close to 0 V vs. SHE with MWCNT, i. e. very close to 0.030 V vs. SHE, the formal redox potential for fumarate/succinate interconversion. A maximum current density in the range of 1 A m À 2 was reached for fumarate reduction. These data highlight a new way to promote direct electron transfer reactions in electroactive artificial biofilms.[a] Dr.

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Engineered Shewanella oneidensis-reduced graphene oxide biohybrid with enhanced biosynthesis and transport of flavins enabled a highest bioelectricity output in microbial fuel cells

Cited by 96 publications

References 78 publications

Internalized Carbon Dots for Enhanced Extracellular Electron Transfer in the Dark and Light

Internalized Carbon Dots for Enhanced Extracellular Electron Transfer in the Dark and Light

Modular engineering to increase intracellular NAD(H/+) promotes rate of extracellular electron transfer of Shewanella oneidensis

Protamine Promotes Direct Electron Transfer Between Shewanella oneidensis Cells and Carbon Nanomaterials in Bacterial Biocomposites

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