Engineered Scaffold Architecture Influences Soft Tissue Regeneration

Wong, Darice Y.; Liao, Elly E.; Lévêque, Jean; Brumblay, Hunter; Lin, Chia Ying; LaMarca, Frank; Krebsbach, Paul H.; Hollister, Scott J.

doi:10.1007/978-0-387-47683-4_5

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…Engineered tissue scaffolds should provide: (1) mechanical support for tissue replacement or regeneration, (2) an environment that favors cell integration, proliferation and cell differentiation for the desired therapeutic effect and (3) a permeable matrix that allows efficient nutrient, gas and waste diffusion [10][11][12]. The chemical composition, physico-mechanical properties (e.g.…”

Section: Introductionmentioning

confidence: 99%

Reduced hydraulic permeability of three-dimensional collagen scaffolds attenuates gel contraction and promotes the growth and differentiation of mesenchymal stem cells

et al. 2010

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Section: Introductionmentioning

confidence: 99%

Reduced hydraulic permeability of three-dimensional collagen scaffolds attenuates gel contraction and promotes the growth and differentiation of mesenchymal stem cells

et al. 2010

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“…to six weeks post injury following Hydrogel ( Fig 5B) and Hydrogel +NT-3 treatment (Fig 5A & B). [49][50][51] However these scaffolds remain rigid and require surgical manipulation to implant and may not be compatible in instances of irregular and misshapen lesions.…”

Section: Hydrogel + Nt-3 Treatment Promotes Neuronal Survival In Vivomentioning

confidence: 99%

Therapeutic Effect of Neurotrophin-3 Treatment in an Injectable Collagen Scaffold Following Rat Spinal Cord Hemisection Injury

Breen¹,

Kraskiewicz²,

Ronan³

et al. 2016

ACS Biomater. Sci. Eng.

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Background. Spinal cord injury (SCI) presents to the clinic as complete, incomplete or compressive. SCI patients also display varying quantities of spinal cord tissue damage or loss. One theory proposed to repair the injured spinal cord and regain motor control is to regenerate axons through the lesion site. Current methods proposed to increase neuroregeneration following SCI include; preserving spared neuronal tissue, increasing axonal regeneration, reducing inflammation, reducing glial scar formation and methods aimed at bridging the lesion gap to facilitate the transmittance of physical cues and provide a platform for neuronal sprouting and functional recovery. Objective. This study was designed to quantify the impact of a local injectable in situ forming hydrogel reservoir therapy following rat hemisection SCI. Method. Our group investigated the effect of hydrogel only treatment following SCI in addition to hydrogels loaded with a neuronal growth factor, Neurotrophin-3 (NT-3), immediately following SCI. Functional recovery, assessed by Basso Beattie Bresnahan (BBB), and local healing mechanism, including neuronal regeneration, neuronal survival, glial scar formation, inflammation, astrocytosis, and collagen deposition were investigated one and six weeks post-surgery. Results. Delivery of an injectable hydrogel increased functional recovery, reduced inhibitory glial scarring and reducing inflammation at the injury site. Similarly hydrogel + NT-3 delivered directly into the injury site reduced glial scarring and collagen deposition resulting in increased neuronal survival across the lesion site. Conclusion. This study represents a novel and effective therapy combining growth factor and a biomaterial based therapy following SCI.

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“…In this study we used poly-ε-caprolactone (PCL) because it is a biodegradable synthetic biomaterial widely tested in neural tissue engineering in vitro studies (45,46) and in studies in vivo of neural damages such as traumatic brain injury (47,48), spinal cord injury (49) and nerve regeneration (50,51).…”

Section: Introductionmentioning

confidence: 99%

One-Dimensional Migration of Olfactory Ensheathing Cells on Synthetic Materials: Experimental and Numerical Characterization

Pérez-Garnés

Martínez‐Ramos

Barcia

et al. 2012

Cell Biochem Biophys

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Olfactory ensheathing cells (OECs) are of great interest for regenerative purposes since they are believed to aid axonal growth. With the view set on strategies to achieve reconnection between neuronal structures it is of greatest importance to characterize the behaviour of these cells on long thread-like structures that may efficiently guide cell spread in a targeted way. Here rat OECs were studied on polycaprolactone long monofilaments, on long bars and on discs. Polycaprolactone turns out to be an excellent substrate for OECs. The cells cover long distances along the monofilaments, and colonize completely these structures. With the help of a one-dimensional analytical model a migration coefficient, a net proliferation rate constant, and the fraction of all cells which undergo migration were obtained. The separate effect of the three phenomena summarized by these parameters on the colonization patterns of the onedimensional paths was qualitatively discussed. Other features of interest were also determined, such as the speed of the advance front of colonization and the order of the kinetics of net cell proliferation. Characterizing migration by means of these quantities may be useful for comparing and predicting features of the colonization process (such as times, patterns, advance fronts, proportion of motile cells) of different cell-substrate combinations.

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Engineered Scaffold Architecture Influences Soft Tissue Regeneration

Cited by 3 publications

References 24 publications

Reduced hydraulic permeability of three-dimensional collagen scaffolds attenuates gel contraction and promotes the growth and differentiation of mesenchymal stem cells

Reduced hydraulic permeability of three-dimensional collagen scaffolds attenuates gel contraction and promotes the growth and differentiation of mesenchymal stem cells

Therapeutic Effect of Neurotrophin-3 Treatment in an Injectable Collagen Scaffold Following Rat Spinal Cord Hemisection Injury

One-Dimensional Migration of Olfactory Ensheathing Cells on Synthetic Materials: Experimental and Numerical Characterization

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