2003
DOI: 10.1128/jvi.77.22.12193-12202.2003
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Engineered Retargeting of Viral RNA Replication Complexes to an Alternative Intracellular Membrane

Abstract: A universal feature of positive-strand RNA viruses is the involvement of host intracellular membranes in RNA replication complex formation and function. This conclusion is based primarily on four observations. First, immunofluorescence and immunoelectron microscopy have localized viral replicase proteins and nascent viral RNA synthesis to intracellular membranes (16,18,24,28,29,33,42,47,51,53,56). Second, in vitro viral RNA-dependent RNA polymerase (RdRp) activity cofractionates with cellular membranes (6,8,12… Show more

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Cited by 78 publications
(95 citation statements)
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References 60 publications
(127 reference statements)
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“…15 However, when Flock House Virus RNA replication complexes were retargeted to the endoplasmic reticulum (ER), the efficiency of viral RNA replication actually increased. 16 These kinds of data suggest that different RNA viruses may co-opt different subcellular compartments for some reason other than the exigencies of replicating RNA, such as to alter cellular signal transduction in ways that are useful during infections of natural hosts.…”
mentioning
confidence: 99%
“…15 However, when Flock House Virus RNA replication complexes were retargeted to the endoplasmic reticulum (ER), the efficiency of viral RNA replication actually increased. 16 These kinds of data suggest that different RNA viruses may co-opt different subcellular compartments for some reason other than the exigencies of replicating RNA, such as to alter cellular signal transduction in ways that are useful during infections of natural hosts.…”
mentioning
confidence: 99%
“…Since LSm1-7, Pat1, and Dhh1 have been shown to localize in P-bodies in close proximity to mitochondria (54), we questioned whether these factors would also affect FHV replication when associated with other membrane compartments. It has been previously shown that FHV RNA replication complexes can be retargeted to the endoplasmic reticulum (ER) through the replacement of the N-proximal sequence by ER-targeting sequences from the HCV NS5B protein (46). These alternative replication complexes were also shown to be fully functional, although FHV replication kinetics were enhanced (46).…”
Section: Lsm1mentioning
confidence: 99%
“…It has been previously shown that FHV RNA replication complexes can be retargeted to the endoplasmic reticulum (ER) through the replacement of the N-proximal sequence by ER-targeting sequences from the HCV NS5B protein (46). These alternative replication complexes were also shown to be fully functional, although FHV replication kinetics were enhanced (46). In these experiments, we focused on protein Dhh1, since it has an effect on the RNA3/RNA1 ratios similar to those of the other two components of the complex without any additional effect on FHV RNA steady-state levels; thus, it shows a clearer phenotype (Fig.…”
Section: Lsm1mentioning
confidence: 99%
“…The FHV RdRp and RCs colocalize to spherules within the outer mitochondrial membrane (32,36,37). The RdRp of a second betanodavirus, AHNV, localizes to mitochondria in infected fish cells and in transfected African green monkey kidney COS-7 cells when expressed from a plasmid in the absence of viral RNA replication, but the significance of this localization for the life cycle of the virus is unknown (38).…”
mentioning
confidence: 99%