Engineered recombinant ovomucoid third domain can desensitize Balb/c mice of egg allergy

Rupa, Prithy; Mine, Yoshinori

doi:10.1111/j.1398-9995.2006.01143.x

Cited by 23 publications

(19 citation statements)

References 24 publications

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“…3A, p,0.05). The total IgE level in allergy mice was lower than those in previous studies (20,27). The above results were consistent with what we had predicted, since oral administration before sensitization suppressed the increase in total IgE (28,29).…”

Section: Discussionsupporting

confidence: 82%

Rush Oral Immunotherapy Does Not Reduce Allergic Response in Mice with Mild Allergy to Egg White Ovomucoid

Maeta

Kaji

Nagaishi

et al. 2015

J Nutr Sci Vitaminol

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Summary Oral immunotherapy (OIT) is a promising therapeutic approach for treating food allergy. Past studies have shown that OIT reduces allergic response only in severe allergy model mice. We worked to establish mild allergy model mice, and investigated whether 'rush' OIT for 10 d improved the allergic response and biomarkers in these mice. Balb/c mice were sensitized to ovomucoid (OM) in alum. The rush OIT was done for 10 d. Oral OM challenge was used to determine the impact of OIT on the allergic response. We measured allergic biomarkers, such as vascular permeability in the skin, plasma levels of total IgE, OM-specific IgE, IgG1 and IgG2a and cytokines in splenocyte culture supernatant. OIT for 10 d did not improve allergy symptoms and increased vascular permeability. Total IgE in the plasma of OIT-treated mice was significantly higher than in that of non-treated mice. OM-specific IgG1 and IgG2a plasma levels were not significantly different between OIT-treated and non-treated mice. Among the cytokine secretion of splenocyte from OITtreated mice, IFN-g and IL-10 were significantly lower than in non-treated mice, and IL-4 and IL-5 were significantly higher. Total TGF-b in the OIT-treated group was not detected. The IFN-g/IL-4 ratio of the OIT-treated group was about 1/8 that of the non-treated group. OIT for 10 d was not effective and some biomarkers showed negative responses in the mild allergy model mice. We suggest OIT should be used very carefully as this treatment carries a risk of worsening allergy symptoms for mice with mild allergy. Key Words oral immunotherapy, food allergy, allergy model mice Food allergies affect about 3% of the overall population and up to 4-6% of children (1-3). In recent times, the prevalence of reported food allergy has increased 18% among children under 18 y of age (2, 3). Dietary avoidance of specific allergens is recommended as the first-line approach in the management of food allergy (1, 2). Recently, oral immunotherapy (OIT) has been studied as a new treatment for food allergy (2,4,5). OIT uses small amounts of food allergen to desensitize the patient. However, OIT is not often recommended because it carries the risk of increasing allergy symptoms (2, 6).In Japan, egg allergy has been reported as the most prevalent food hypersensitivity in the pediatric population, exceeding cow's milk allergy (7). Ovalbumin (OVA) and ovomucoid (OM) were identified as egg white allergen epitopes, constituting about 54 and 11% of egg white protein, respectively (8,9). OM is heat resistant and has high allergenicity (10, 11). It is a well-known trypsin inhibitor (8). This suggested that OIT for OM (egg)-specific allergy patients may be a highly effective treatment and a pathway to allergen tolerance.As research in this area has been limited, there is inadequate evidence to establish proper OIT protocols, such as allergen dosage, escalation, duration of therapy and method of administration (2). OIT protocols are categorized as either 'slow' or 'rush.' In slow OIT, the allergen dose is increas...

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Section: Discussionsupporting

confidence: 82%

Rush Oral Immunotherapy Does Not Reduce Allergic Response in Mice with Mild Allergy to Egg White Ovomucoid

Maeta

Kaji

Nagaishi

et al. 2015

J Nutr Sci Vitaminol

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“…Although murine peanut allergy models have been reported to accurately resemble human anaphylaxis [20], the clinical symptom scores were lower in BALB/c [20] and C3H/HeOuJ mice [30] receiving peanut orally than systemically. As shown elsewhere, intraperitoneal sensitization has proven successful [25,31], food allergens have been widely used in models of systemic anaphylaxis [14,32,33]; both classic and alternative pathways were activated in a mouse model of systemic sensitization [8]. As our primary aim was to study systemic anaphylaxis (activating IgE and IgG pathways) expressed by sharp clinical and immunological responses in both strains, we developed a systemic sensitization protocol.…”

Section: Discussionmentioning

confidence: 99%

Differences in the Anaphylactic Response between C3H/HeOuJ and BALB/c Mice

Martín

Hernández²,

Torre³

et al. 2017

Int Arch Allergy Immunol

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Background: Anaphylaxis is a severe and potentially lethal allergic reaction whose incidence is increasing. Murine models can elucidate the underlying mechanisms and pave the way for appropriate therapeutic options. However, differences in strains and protocols hamper comparisons of data between researchers. We performed a parallel study of clinical and immune responses with 2 strains of mice, BALB/c and C3H/HeOuJ, in an allergen-induced systemic anaphylaxis protocol. Both strains have been widely used in allergy models, although they have not been compared in an intraperitoneal systemic model. Methods: Groups of 5-week-old female BALB/c and C3H/HeOuJ mice were intraperitoneally sensitized with peanut in the presence of adjuvants. Specific immunoglobulin (sIg) G1, sIgG2a, sIgE, total IgE, histamine release, and specific stimulated splenocyte cytokines, interleukin (IL)-4, IL-5, IL-10, IL-12, IL-13, and interferon (IFN)-γ, were assessed. At week 6, mice were intraperitoneally challenged with peanut. Anaphylaxis was evaluated by recognition of clinical symptoms and changes in body temperature. Results: All peanut-sensitized mice induced sIg and developed anaphylactic symptoms upon challenge. Nonetheless, the C3H/HeOuJ strain demonstrated earlier and persistently higher sIgG1 and sIgG2a production, elevated sIgE, and more severe clinical symptoms and histamine release than the BALB/c strain. In contrast, BALB/c exhibited higher release of IL-4, IL-5, IL-10, IL-13, and IFN-γ. Conclusions: Both models are suitable for studying anaphylaxis. Consequently, they could be used in research on the pathogenesis and therapy of anaphylaxis. However, according to the type of study performed, differences in the specific clinical, humoral, and cellular responses to antigens have to be considered.

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“…Actually our previous studies demonstrated that various allergens such as ovalbumin, tropomyosin, α-lactoglobulin, β-lactoglobulin, and casein structurally modified by gamma irradiation reduced a binding ability to IgE of allergic patients [5][6][7]10]. Their properties might be applied to manufacture of functional foods for allergic patients [7].…”

Section: Discussionmentioning

confidence: 97%

Ovalbumin modified by gamma irradiation alters its immunological functions and allergic responses

Seo

Kim

Lee

et al. 2007

International Immunopharmacology

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Engineered recombinant ovomucoid third domain can desensitize Balb/c mice of egg allergy

Cited by 23 publications

References 24 publications

Rush Oral Immunotherapy Does Not Reduce Allergic Response in Mice with Mild Allergy to Egg White Ovomucoid

Rush Oral Immunotherapy Does Not Reduce Allergic Response in Mice with Mild Allergy to Egg White Ovomucoid

Differences in the Anaphylactic Response between C3H/HeOuJ and BALB/c Mice

Ovalbumin modified by gamma irradiation alters its immunological functions and allergic responses

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