Engineered proteins with sensing and activating modules for automated reprogramming of cellular functions

Sun, Jie; Lei, Lei; Tsai, Chuen-Tinn; Wang, Yi; Shi, Yiwen; Ouyang, Mingxing; Lu, Shaoying; Kim, Tae-Jin; Wang, Pengzhi; Huang, Min; Xu, Xiangdong; Nizet, Victor; Chien, Shu; Wang, Yingxiao

doi:10.1038/s41467-017-00569-6

Cited by 33 publications

(29 citation statements)

References 43 publications

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“…The iSNAPS protein is inserted into macrophages in the study, and it reconnects macrophages, covering the escape signals recognized by the tumor cells and interpreting them as phagocytic signals. In addition, its rapid response and strong lethality can significantly enhance the ability of macrophages to divide, phagocytose, and kill tumor cells rapidly (84).…”

Section: Isnaps Smart Protein Moleculesmentioning

confidence: 99%

“…The design principle of this intelligent protein molecule can also be used to redesign other immune cells for cancer treatment. At present, the team plans to test iSNAPS in mice and may study its application in other areas (84). This protein may influence not only tumor treatment but also other diseases and self-regulation, and further research is needed.…”

Section: Isnaps Smart Protein Moleculesmentioning

confidence: 99%

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Tumor-Associated Macrophages: Recent Insights and Therapies

Zhou

Tang²,

Gao³

et al. 2020

Front. Oncol.

460

373

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Macrophages, which have functions of engulfing and digesting foreign substances, can clear away harmful matter, including cellular debris and tumor cells. Based on the condition of the internal environment, circulating monocytes give rise to mature macrophages, and when they are recruited into the tumor microenvironment and in suitable conditions, they are converted into tumor-associated macrophages (TAMs). Generally, macrophages grow into two main groups called classically activated macrophages (M1) and alternatively activated macrophages (M2). M2 and a small fraction of M1 cells, also known as TAMs, not only lack the function of phagocytizing tumor cells but also help these tumor cells escape from being killed and help them spread to other tissues and organs. In this review, we introduce several mechanisms by which macrophages play a role in the immune regulation of tumor cells, including both killing factors and promoting effects. Furthermore, the targeted therapy for treating tumors based on macrophages is also referred to in our review. We confirm that further studies of macrophage-focused therapeutic strategies and their use in clinical practice are needed to verify their superior efficacy and potential in cancer treatment.

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Section: Isnaps Smart Protein Moleculesmentioning

confidence: 99%

Section: Isnaps Smart Protein Moleculesmentioning

confidence: 99%

Tumor-Associated Macrophages: Recent Insights and Therapies

Zhou

Tang²,

Gao³

et al. 2020

Front. Oncol.

460

373

View full text Add to dashboard Cite

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“…Utilizing this imaging and analysis protocols, our group has published an array of papers engineering FRET biosensors and quantifying the time courses of dynamic molecular activities for kinases, proteases, and membrane channels in live cells [6,23,24,26,28,40,53,54,56]. Currently, we do not correct for multi-channel cross-talks and bleed-through between fluorophores or estimate pixelwise FRET efficiency by default [57].…”

Section: Discussionmentioning

confidence: 99%

Fluocell for Ratiometric and High-Throughput Live-Cell Image Visualization and Quantitation

et al. 2019

Self Cite

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Spatiotemporal regulation of molecular activities dictates cellular function and fate. Investigation of dynamic molecular activities in live cells often requires the visualization and quantitation of fluorescent ratio image sequences with subcellular resolution and in high throughput. Hence, there is a great need for convenient software tools specifically designed with these capabilities. Here we describe a well-characterized open-source software package, Fluocell, customized to visualize pixelwise ratiometric images and calculate ratio time courses with subcellular resolution and in high throughput. Fluocell also provides group statistics and kinetic analysis functions for the quantified time courses, as well as 3D structure and function visualization for ratio images. The application of Fluocell is demonstrated by the ratiometric analysis of intensity images for several single-chain Förster (or fluorescence) resonance energy transfer (FRET)-based biosensors, allowing efficient quantification of dynamic molecular activities in a heterogeneous population of single live cells. Our analysis revealed distinct activation kinetics of Fyn kinase in the cytosolic and membrane compartments, and visualized a 4D spatiotemporal distribution of epigenetic signals in mitotic cells. Therefore, Fluocell provides an integrated environment for ratiometric live-cell image visualization and analysis, which generates high-quality single-cell dynamic data and allows the quantitative machine-learning of biophysical and biochemical computational models for molecular regulations in cells and tissues.

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“…Moving forward, the team is planning to test the iSNAPs in mice to see how they could perform in vivo. The researchers are also interested in studying whether the iSNAPs can be harnessed to enhance other native cellular functions to correct various disease pathologies [7].…”

Section: Gerontol and Geriatric Stud Gerontology And Geriatrics Studiesmentioning

confidence: 99%

Protein and Cholesterol's Role in the Global Battle against World Cancer Epidemic

Skopec

2017

GGS

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As an ancillary proposition we can note that tumors are more than insular masses of proliferating cancer cells. Instead they are complex tissues composed of multiple distinct cell types that participate in heterotypic interactions with one another. There are the recruited normal cells, which form tumor-associated stroma, as active participants in tumorigenesis rather than passive bystanders. As such, these stromal cells contribute to the development and expressing of certain hallmark capabilities. During the last decades this notion has been solidified and extended, revealing that the biology of tumors can no longer be understood simply by enumerating the traits of the cancer cells but instead must encompass the contributions of the tumor microenvironment to World Cancer Epidemic.

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Engineered proteins with sensing and activating modules for automated reprogramming of cellular functions

Cited by 33 publications

References 43 publications

Tumor-Associated Macrophages: Recent Insights and Therapies

Tumor-Associated Macrophages: Recent Insights and Therapies

Fluocell for Ratiometric and High-Throughput Live-Cell Image Visualization and Quantitation

Protein and Cholesterol's Role in the Global Battle against World Cancer Epidemic

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