Engineered Polyelectrolyte Multilayer Substrates for Adhesion, Proliferation, and Differentiation of Human Mesenchymal Stem Cells

Semenov, Oleg V.; Malek, Antoine; Bittermann, Anne Greet; Vörös, János; Zisch, Andreas H.

doi:10.1089/ten.tea.2008.0602

Cited by 71 publications

(70 citation statements)

References 33 publications

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“…[2][3][4][5] Ease of preparation and extreme versatility make them great candidates for the design of functional and responsive coatings for biomedical technologies, 6 microcapsules for drug delivery, 7 substrates for cell adhesion and proliferation, 8 solid-state light-emitting devices. 9 However, the simplicity of PEMs assembly contrasts with the complex interplay of parameters which determines film growth and structure.…”

Section: Introductionmentioning

confidence: 99%

Short versus long chain polyelectrolyte multilayers: a direct comparison of self-assembly and structural properties

Micciulla

Dodoo

Chevigny

et al. 2014

Phys. Chem. Chem. Phys.

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a Successful layer-by-layer (LbL) growth of short chain (B30 repeat units per chain) poly(sodium styrene sulfonate) (PSS)-poly(diallyl dimethylammonium chloride) (PDADMAC) multilayers is presented for the first time and compared with the growth of equivalent long chain polyelectrolyte multilayers (PEMs). A detailed study performed by quartz crystal microbalance with dissipation (QCM-D) is carried out and three main processes are identified: (i) initial mass uptake, (ii) adsorption-desorption during layer equilibration and (iii) desorption during rinsing. In contrast to the high stability and strong layer increment of high molecular weight (HMW) PEMs, layer degradation characterizes low molecular weight (LMW) multilayers. In particular, two different instability phenomena are observed: a constant decrease of sensed mass during equilibration after PDADMAC adsorption, and a strong mass loss by salt-free rinsing after PSS adsorption. Yet, an increase of salt concentration leads to much stronger layer growth.First, when the rinsing medium is changed from pure water to 0.1 M NaCl, the mass loss during rinsing is reduced, irrespective of molecular weight. Second, an increase in salt concentration in the LMW PE solutions causes a larger increment during the initial adsorption step, with no effect on the rinsing.Finally, the mechanical properties of the two systems are extracted from the measured frequency and dissipation shifts, as they offer a deeper insight into the multilayer structures depending on chain length and outermost layer. The paper enriches the field of PE assembly by presenting the use of very short PE chains to form multilayers and elucidates the role of preparation conditions to overcome the limitation of layer stability.

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Section: Introductionmentioning

confidence: 99%

Short versus long chain polyelectrolyte multilayers: a direct comparison of self-assembly and structural properties

Micciulla

Dodoo

Chevigny

et al. 2014

Phys. Chem. Chem. Phys.

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“…The characteristic protein of the extracellular matrix, fibronectin, was adsorbed to the surface. The fibronectin was prepared with a final concentration of 50 mg/ mL [9][10][11][12][13]. …”

Section: Porous Synthetic Coatingsmentioning

confidence: 99%

Development of Surface Modification Methods for Religaheart® Cardiac Support System

Major¹,

Kustosz²,

Trembecka-Wójciga³

et al. 2016

Archives of Metallurgy and Materials

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The work is a review of the methods of the surface modification performed by the authors dedicated for for cardiac support system. It presents the evolution of designing the surface dedicated to direct contact with blood. Initially thin and ultrathin coatings were developed. They were designed as a blood-polymer barrier. The pneumatic heart assist devices are made of a medical grade polyurethane. A major milestone was to create advanced ceramic thin films expressing the flexible effects deposited by physical techniques. Coatings have evolved. Another milestone was the surface reproducing the microenvironment to capture progenitor cells from the bloodstream. Thin coatings were prepared, using methods of ion been, controlled residual stresses were introduced. Wrinkles appeared without cracking. This enabled taking control over the process of cell differentiation. Alternatively, the tissue inspired structure resulted of the coating in the form of extracellular matrix. The outer surface was modified with synthetic materials. This enabled the effective proteins docking to induce cell growth, recreating the luminal side of the blood vessel. Coagulation processes have been slowed down. In addition, it was found pro-angiogenic effect.

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“…Due to WJ-MSCs' unique immuno-modulatory characteristics, such scaffold-free structures even could have potential for use in allogenic transplantations. Currently, the first attempts to use perinatal placenta-derived stem cells (PD-MSCs) for the manufacturing of live, differentiable cell sheets have been successfully demonstrated [78,79].…”

Section: Advantages and Limitations For The Use Of Wj-mscs In Tissue mentioning

confidence: 99%

Mesenchymal Stem Cells Derived from Wharton's Jelly and their Potential for Cardio-Vascular Tissue Engineering

Semenov¹

2011

TOTERMJ

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Abstract:Experimental results accumulated during last decade suggest that human perinatal tissues such as placenta, fetal membranes, and umbilical cord, as well as perinatal fluids such as, amniotic fluid and umbilical cord blood, harbour different amounts of multipotent precursor cells, called extra-embryonic mesenchymal stem cells (EE-MSCs). Perinatal EE-MSCs represent an intermediate cell type between pluripotent embryonic stem cells (ESCs) and multipotent MSCs derived from variety of postnatal human tissues, such as bone marrow, fat, dental pulp, etc. Multipotent mesenchymal cells obtained from connective Wharton s Jelly tissue of umbilical cord (WJ-MSCs) currently emerged as particularly interesting type of perinatal EE-MSCs, related for therapeutical applications and cryobanking. These cells are easily assessable for isolation, possess fetal karyotype, and hold very active growth potential. Due to their unique developmental position WJ-MSCs exhibit specific phenotype which combines some markers expressed by postnatal bone marrowderived MSCs, such as CD73, CD90, and CD105 with some markers typically expressed by ESCs, such as Oct-3/4, Sox-2, and Nanog. In terms of plasticity WJ-MSCs demonstrate the potential for differentiation towards mesodermal and ectodermal lineages in vitro and in vivo. Additionally, extraembryonic tissues are normally discarded after birth and the isolation of WJ-MSCs is free of ethical concerns. In this work we aim to review previously obtained experimental results, discuss different aspects concerning plasticity and immunomodulatory characteristics of WJ-MSCs, and evaluate the potential of these cells for biomedical and clinical applications.

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Engineered Polyelectrolyte Multilayer Substrates for Adhesion, Proliferation, and Differentiation of Human Mesenchymal Stem Cells

Cited by 71 publications

References 33 publications

Short versus long chain polyelectrolyte multilayers: a direct comparison of self-assembly and structural properties

Short versus long chain polyelectrolyte multilayers: a direct comparison of self-assembly and structural properties

Development of Surface Modification Methods for Religaheart® Cardiac Support System

Mesenchymal Stem Cells Derived from Wharton's Jelly and their Potential for Cardio-Vascular Tissue Engineering

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