Engineered native pathways for high kaempferol and caffeoylquinate production in potato

Rommens, Caius M.; Richael, Craig; Hua, Yan; Navarre, Duroy A.; Ye, Jingsong; Krucker, Michele; Swords, Kathy

doi:10.1111/j.1467-7652.2008.00362.x

Cited by 75 publications

(66 citation statements)

References 67 publications

(59 reference statements)

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“…Vertical bars indicate the standard deviation of three biological replicates. Asterisks indicate a significant difference at the P \ 0.05 level Plant Cell Tiss Organ Cult increased expression of phenylpropanoid synthesis related genes (Rommens et al 2008). We further analyzed whether the increased caffeoylquinic acid contents in transgenic plants overexpressing SbMYB8 was due to the changed expression of flavonoid biosynthesis-related genes.…”

Section: Discussionmentioning

confidence: 99%

A Scutellaria baicalensis R2R3-MYB gene, SbMYB8, regulates flavonoid biosynthesis and improves drought stress tolerance in transgenic tobacco

Yuan

Yang

et al. 2014

Plant Cell Tiss Organ Cult

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R2R3-MYB proteins are involved in the primary and secondary metabolism, developmental processes and the responses to biotic and abiotic stresses. Little is known about the functions of R2R3-MYB proteins in Scutellaria baicalensis Georgi which is a traditional Chinese medicinal plants. In this study, the function of a S. baicalensis R2R3-MYB protein, SbMYB8, was investigated. SbMYB8 had similar expression pattern with SbC4H and SbCHS in ABA-treated S. baicalensis, indicating that SbMYB8 might be involved in the flavonoid metabolism. SbMYB8 protein could bind to the GmMYB92 BS3 sequence of SbCHS promoter region, regulating the expression of SbCHS. The SbMYB8 protein was localized to the nucleus where it activated transcription. The transgenic tobacco plants over-expressing SbMYB8 had higher caffeoylquinic acid contents, compared to that in wild type plants. Overexpression of SbMYB8 also changed the expression level of some flavonoid biosynthesis-related genes. It was found that overexpression of SbMYB8 can improve stress tolerance of transgenic plants, and can alter the activity and expression levels of some antioxidant enzymes. These results indicate that SbMYB8 plays important roles in flavonoid biosynthesis and stress tolerance of plant.

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Section: Discussionmentioning

confidence: 99%

A Scutellaria baicalensis R2R3-MYB gene, SbMYB8, regulates flavonoid biosynthesis and improves drought stress tolerance in transgenic tobacco

Yuan

Yang

et al. 2014

Plant Cell Tiss Organ Cult

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“…However, further biochemical analysis will be necessary to determine if this overall modulation of the phenylpropanoid pathway is directed primarily toward an increased production of scopoletin and its glucosylated derivative scopolin that accumulate during PPD (Wheatley and Schwabe, 1985;Buschmann et al, 2000b). Previous studies demonstrated the antioxidant activity of free scopoletin as well as the phenylpropanoid caffeoyl quinate (chlorogenic acid), which is produced by p-coumaroyl shikimate/quinate 39-hydroxylase (Chong et al, 1999;Niggeweg et al, 2004;Rommens et al, 2008). Thus, phenylpropanoid pathway modulation might also reflect a response to ROS production during PPD (Reilly et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Large-Scale Proteomics of the Cassava Storage Root and Identification of a Target Gene to Reduce Postharvest Deterioration

et al. 2014

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ORCID IDs: 0000-0003-2102-9737 (H.V.); 0000-0002-1904-9440 (K.B.) Cassava (Manihot esculenta) is the most important root crop in the tropics, but rapid postharvest physiological deterioration (PPD) of the root is a major constraint to commercial cassava production. We established a reliable method for image-based PPD symptom quantification and used label-free quantitative proteomics to generate an extensive cassava root and PPD proteome. Over 2600 unique proteins were identified in the cassava root, and nearly 300 proteins showed significant abundance regulation during PPD. We identified protein abundance modulation in pathways associated with oxidative stress, phenylpropanoid biosynthesis (including scopoletin), the glutathione cycle, fatty acid a-oxidation, folate transformation, and the sulfate reduction II pathway. Increasing protein abundances and enzymatic activities of glutathione-associated enzymes, including glutathione reductases, glutaredoxins, and glutathione S-transferases, indicated a key role for ascorbate/glutathione cycles. Based on combined proteomics data, enzymatic activities, and lipid peroxidation assays, we identified glutathione peroxidase as a candidate for reducing PPD. Transgenic cassava overexpressing a cytosolic glutathione peroxidase in storage roots showed delayed PPD and reduced lipid peroxidation as well as decreased H 2 O 2 accumulation. Quantitative proteomics data from ethene and phenylpropanoid pathways indicate additional gene candidates to further delay PPD. Cassava root proteomics data are available at www.pep2pro.ethz.ch for easy access and comparison with other proteomics data.

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“…Neochlorogenic and cryptochlorogenic acids were quantitated as chlorogenic acid equivalents (Shakya and Navarre 2006). Flavonols were determined as previously described and kaempferol-rutinose was expressed as rutin equivalents (Rommens et al 2008). Authentic standards for kaempferol-rutinose were not available, so tentative identification was based on the uv spectrum and a pseudomolecular ion of m/z 593 (M-H) − and a MS 2 product ion of m/z 285 (M-H-rutinose) − .…”

Section: Hplc Analysismentioning

confidence: 99%

“…Furthermore, some wild species exhibit less enzymatic browning despite higher phenolic concentrations than S. tuberosum cultivars (Sim et al 1997). Transgenic approaches have shown that it is possible to markedly increase tuber phenolic acids, anthocyanins and flavonols (Lukaszewicz et al 2004;Rommens et al 2008). Chlorogenic acid is bioavailable in humans (Monteiro et al 2007), may decrease the risk of type two diabetes (Legrand and Scheen 2007) and slow the release of glucose into the bloodstream (Bassoli et al 2008), which could be relevant for lowering the glycemic index value of potatoes.…”

Section: Introductionmentioning

confidence: 97%

The Effect of Different Cooking Methods on Phenolics and Vitamin C in Developmentally Young Potato Tubers

et al. 2010

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New potatoes (also known as "baby potatoes") are harvested at a young developmental age and have higher concentrations of some phytonutrients than mature tubers. Assessing the potential of new potatoes in the diet and their suitability as targets for further nutritional enhancement requires knowledge of how well targeted phytonutrients survive cooking. New potatoes from the cultivars 'Piccolo,' 'Bintje' and 'Purple Majesty' were microwaved, baked, boiled, steamed or stir-fried. LCMS analysis showed that total phenolics, chlorogenic acids, flavonols and vitamin C did not significantly decrease after cooking by any of these methods. Cooking typically resulted in an increase in the recoverable amounts of these compounds, with chlorogenic acid increasing from 2.14 to 2.92 mg/g DW and rutin from 7.4 to 13.2 μg/g DW after baking in 'Purple Majesty.' Supporting this finding is that antioxidant capacity showed similar results with Trolox equivalents increasing from 28.7 (raw) to 36.3 µmol/g DW after baking in 'Bintje.' These data suggest that new potatoes present an opportunity to more fully utilize the nutritional potential of potatoes. This work shows that significant loses of phenolic acids, vitamin C, and flavonols need not occur during cooking and confirms that these are viable targets to further increase by molecular or breeding approaches.Resumen Papas nuevas (también conocidas como "papas bebe"), se cosechan en una edad temprana de desarrollo y tienen concentraciones más altas de algunos fitonutrientes que los tubérculos maduros. El análisis del potencial de papas nuevas en la dieta y su ubicación como blancos de incremento nutricional adicional, requiere del conocimiento de qué tan bien los fitonutrientes de estudio sobrevivan al cocinado. Papas nuevas de los cultivares "Piccolo", "Bintje" y "Purple Majesty" pasaron por microondas, puré, hervido, al vapor o al freído. El análisis de LCMS mostró que los fenoles totales, los ácidos clorogénicos, flavonoides y vitamina C, no disminuyeron significativamente después del cocinado por cualquiera de estos métodos. El cocinado típico dio por resultado un aumento en las cantidades recobrables de estos compuestos, con un aumento del ácido clorogénico de 2.14 a 2.92 mg/g en peso seco y de rutina de 7.4 a 13.2 μg/g de peso seco después del cocinado en puré de "Purple Majesty". En respaldo a este resultado es que la capacidad antioxidante mostró resultados similares con equivalentes Trolox aumentando de 28.7 (crudo) a 36.3 µmol/g de peso seco después del cocinado como puré en "Bintje". Estos datos sugieren que las papas nuevas presentan una oportunidad para una utilización más completa del potencial nutritivo de las papas. Este trabajo muestra que las pérdidas significativas de ácidos fenólicos, vitamina C, y flavonoides no necesariamente se tienen que presentar durante el cocinado, y confirma que estos son blancos viables para un aumento posterior por estrategias moleculares o de mejoramiento.

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Engineered native pathways for high kaempferol and caffeoylquinate production in potato

Abstract: Summary

Cited by 75 publications

References 67 publications

A Scutellaria baicalensis R2R3-MYB gene, SbMYB8, regulates flavonoid biosynthesis and improves drought stress tolerance in transgenic tobacco

A Scutellaria baicalensis R2R3-MYB gene, SbMYB8, regulates flavonoid biosynthesis and improves drought stress tolerance in transgenic tobacco

Large-Scale Proteomics of the Cassava Storage Root and Identification of a Target Gene to Reduce Postharvest Deterioration

The Effect of Different Cooking Methods on Phenolics and Vitamin C in Developmentally Young Potato Tubers

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