Engineered nanoparticles induce cell apoptosis: potential for cancer therapy

Ma, Dandan; Yang, Wan-Xi

doi:10.18632/oncotarget.8553

Cited by 79 publications

(37 citation statements)

References 112 publications

(124 reference statements)

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“…Additionally, intrinsic or extrinsic pathways of apoptosis induced by AgNPs have been demonstrated in several studies. [25][26][27][28] Mitochondrial and proapoptotic proteins mediate the intrinsic apoptotic pathway, 29,30 and our results clearly indicate that apoptosis was induced by CC-AgNPs via involvement of a mitochondria-mediated pathway in A549 cells. Subsequent apoptosis and loss of mitochondrial membrane potential is mediated by the Bcl2 protein family.…”

Section: Discussionsupporting

confidence: 58%

Biosynthesis, characterization, and anticancer effect of plant-mediated silver nanoparticles using Coptis chinensis

Pei¹,

Fu²,

Jiang³

et al. 2019

IJN

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Background Tremendous growth in nanotechnology has opened up new frontiers in fundamental and applied aspects, including the synthesis of nanoscale matter and understanding/utilizing its exotic physicochemical and optoelectronic properties. Green-synthesis methods employing either biological microorganisms or plant extracts have emerged as a simple and alternative to chemical synthesis. Methods In our present study, we aimed to synthesize silver nanoparticles (AgNPs) in combination with an aqueous extract of Coptis chinensis (CC) using a suitable ecofriendly green-synthesis way. Results In our results, ultraviolet-visible spectroscopy revealed a near-absorbance peak at 450 nm, which confirmed the AgNP synthesis. The crystalline nature of the AgNPs was revealed with X-ray diffraction. Transmission electron-microscopy analysis showed spherically dispersed nanoparticles of 6–45 nm diameter. We analyzed the elementary mechanism across A549 lung carcinoma cells ahead of treatment with doses of CC-AgNPs (10 µg/mL and 25 µg/mL). The antiproliferative effect of CC-AgNPs revealed a significant decline in cell viability. Antibacterial assays with both Gram-negative ( Escherichia coli ) and Gram-positive ( Staphylococcus aureus ) bacteria exhibited a higher zone of inhibition against S. aureus . Conclusion Furthermore, CC-AgNPs regulated apoptosis using the intrinsic pathway to inhibit A549-cell proliferation. Proliferation migration and invasion were notably inhibited by CC-AgNPs, which promoted apoptosis in lung adenocarcinoma cells by regulating the apoptotic pathway.

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Section: Discussionsupporting

confidence: 58%

Biosynthesis, characterization, and anticancer effect of plant-mediated silver nanoparticles using Coptis chinensis

Pei¹,

Fu²,

Jiang³

et al. 2019

IJN

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“…8, exposing cells to ZnO nanoparticles caused an increase in membrane disruption and formation of lysosomes vacuoles compared to untreated control cells. The excessive capacity to cause dying to the cell is associated with the capability of nanoparticle to penetrate via the cellular membrane and impact at the mRNA expression of suppression gene that cause increase in the production level of reactive oxygen species (ROS) within the cell [21]. With expanded levels of ROS and oxidative stress, ZnO NPs display a deleterious impact at the lipid, protein and nucleic acid of the cell [22].…”

Section: Control Cell Zno Nanoparticles Treated Cellsmentioning

confidence: 99%

Zinc Oxide Nanoparticles Induces Apoptosis in Human Breast Cancer Cells via Caspase-8 and P53 Pathway

Kadhem¹,

Ibraheem²,

Jabir³

et al. 2019

Nano BioMed ENG

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Nanoparticles are a special institution of substances with precise capabilities and significant applications in many biomedical fields. In the present work zinc oxide nanoparticles were prepared through sol-gel approach. The synthesised nanoparticles were identified through the usage of X-ray powder diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). In-vitro anticancer activity of zinc oxide nanoparticles towards MCF-7 cell lines using numerous parameters was investigated. Zinc oxide nanoparticles were determined to exert cell growth arrest against MCF-7 cell lines. The anti-proliferative efficiency of ZnO nanoparticles was due to cell dying and inducing apoptosis that were confirmed by the usage of acridine orange/ethidium bromide dual staining, DAPI staining and genotoxicity assay. Reverse transcription polymerase chain reaction (RT-PCR) analysis achieved to identify the gene expression of Caspase-8, Caspase-9, and P53. The results suggested that ZnO nanoparticles might find a wide use in clinical applications and provide new drug recompense for chemotherapy drugs.

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“…Cell death and apoptosis (e.g. mitochondrial fragmentation/apoptosis, phosphorylation of p53, death receptor, cleavage of caspase and PARP pathways) processes are highly determined by exposure dose and in vitro characteristics such as cell line and tissue (Ma and Yang 2016;De Stefano, Carnuccio and Maiuri 2012). Cytoskeleton organization (e.g.…”

Section: Discussionmentioning

confidence: 99%

Application of Bayesian networks in determining nanoparticle-induced cellular outcomes using transcriptomics

et al. 2019

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Inroads have been made in our understanding of the risks posed to human health and the environment by nanoparticles (NPs) but this area requires continuous research and monitoring. Machine learning techniques have been applied to nanotoxicology with very encouraging results. This study deals with bridging physicochemical properties of NPs, experimental exposure conditions and in vitro characteristics with biological effects of NPs on a molecular cellular level from transcriptomics studies. The bridging is done by developing and implementing Bayesian Networks (BNs) with or without data preprocessing. The BN structures are derived either automatically or methodologically and compared. Early stage nanotoxicity measurements represent a challenge, not least when attempting to predict adverse outcomes and modeling is critical to understanding the biological effects of exposure to NPs. The preprocessed data-driven BN showed improved performance over automatically structured BN and the BN with unprocessed datasets. The prestructured BN captures inter relationships between NP properties, exposure condition and in vitro characteristics and links those with cellular effects based on statistic correlation findings. Information gain analysis showed that exposure dose, NP and cell line variables were the most influential attributes in predicting the biological effects. The BN methodology proposed in this study successfully predicts a number of toxicologically relevant cellular disrupted biological processes such as cell cycle and proliferation pathways, cell adhesion and extracellular matrix responses, DNA damage and repair mechanisms etc., with a success rate >80%. The model validation from independent data shows a robust and promising methodology for incorporating transcriptomics outcomes in a hazard and, by extension, risk assessment modeling framework by predicting affected cellular functions from experimental conditions.

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Engineered nanoparticles induce cell apoptosis: potential for cancer therapy

Cited by 79 publications

References 112 publications

<p>Biosynthesis, characterization, and anticancer effect of plant-mediated silver nanoparticles using <em>Coptis chinensis</em></p>

<p>Biosynthesis, characterization, and anticancer effect of plant-mediated silver nanoparticles using <em>Coptis chinensis</em></p>

Zinc Oxide Nanoparticles Induces Apoptosis in Human Breast Cancer Cells via Caspase-8 and P53 Pathway

Application of Bayesian networks in determining nanoparticle-induced cellular outcomes using transcriptomics

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