Engineered minimal type I CRISPR-Cas system for transcriptional activation and base editing in human cells
Jing Guo,
Luyao Gong,
Haiying Yu
et al.
Abstract:Type I CRISPR-Cas systems are widespread and have exhibited remarkable versatility and efficiency in genome editing and gene regulation in prokaryotes. However, due to the multi-subunit composition and large size, their application in eukaryotes has not been thoroughly investigated. Here, we demonstrate that the type I-F2 Cascade, the most compact among type I systems and significantly smaller than SpCas9, can be developed into programmable tools for use in human cells. For transcriptional activation, the effi… Show more
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