Engineered Microtissues Formed by Schiff Base Crosslinking Restore the Chondrogenic Potential of Aged Mesenchymal Stem Cells

Millan, Christopher; Cavalli, Emma; Groth, Thomas; Maniura‐Weber, Katharina; Zenobi‐Wong, Marcy

doi:10.1002/adhm.201500102

Cited by 25 publications

(32 citation statements)

References 55 publications

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“…However, the different ability of these composite gels to promote chondrogenic differentiation of encapsulated BMSCs found in this study, might be due to the different quantity of PRP, which is correlated with the amount of growth factors loaded, as has been also shown by others (Millan, Cavalli, Groth, Maniura‐Weber, & Zenobi‐Wong, ). Millan et al () found that addition of TGF‐β3 in engineered microtissues (EMTs) greatly influenced the chondrogenic differentiation of the encapsulated MSCs. The EMTs loaded with either 50 or 75 ng of TGF‐β3 expressed higher amounts of sulfated glycosaminoglycan and more Type II collagen than the samples loaded with 25 ng of TGF‐β3, only.…”

Section: Discussionsupporting

confidence: 50%

Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair

Gao

Groth

et al. 2019

J Biomedical Materials Res

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Three‐dimensional scaffolds like hydrogels can be employed as cell carriers for in vitro or in vivo colonization and have become a major research topic to replace damaged tissue. In the current study, a novel composite hydrogel composed of sodium alginate (SA) and platelet‐rich‐plasma (PRP) varying in blending ratios, cross‐linked with calcium ions, released from calcium carbonate‐D‐Glucono‐d‐lactone (CaCO3‐GDL) was successfully prepared. It was found that addition of PRP changed largely the physical properties and biological performance of the composite hydrogels, which was depending on the blending ratio. The gelation rate and swelling ratio of alginate hydrogels were significantly reduced by the addition of PRP, which produced also a more homogeneous gel structure. Field emission scanning electron microscopy (FE‐SEM) investigation confirmed the incorporation of PRP‐derived proteins in the hydrogel, where a porous structure with a pore size of 200–300 μm was found. On the other hand, an increase in surface roughness was observed after the addition of PRP. The compressive mechanical strength of SA/PRP composite hydrogel was enhanced in comparison to the pure SA gel. The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa. It was also found that the in vitro degradation of the alginate gel was accelerated by the addition of PRP. In terms of cellular responses, all gels exhibited an excellent cytocompatibility. Indeed, the composite hydrogels supported bone marrow‐derived mesenchymal stem cells proliferation and their chondrogenesis with up‐regulation of chondrogenic marker genes Sox9 and Aggrecan. Overall, the present study suggests a great potential of SA/PRP composite hydrogels as cell carriers for cartilage tissue engineering.

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Section: Discussionsupporting

confidence: 50%

Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair

Gao

Groth

et al. 2019

J Biomedical Materials Res

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“…of studiesReferencesCell SourceNo. of studies a ReferencesHuman73[48, 50, 52, 53, 168–236]Bone marrow62[48, 50–53, 164, 168, 170–173, 177–180, 182–185, 187, 188, 192, 195–197, 203, 206–210, 212, 216, 217, 219, 221, 223, 227, 230, 232–235, 237–255]Rabbit17[240–242, 246, 249, 252, 255–265]Adipose36[66, 169, 175, 176, 181, 186, 189, 193, 194, 199, 201, 202, 211, 214, 216, 218–220, 224, 228, 229, 231, 235, 242, 256, 257, 260–269]Bovine5[51, 164, 243, 245, 270]Synovium9[174, 191, 200…”

Section: Resultsmentioning

confidence: 99%

“…of studies (%)ReferencesGrowth factorNo. of studies (%)ReferencesTGF-β148 (44%)[50, 169–175, 189, 190, 192, 193, 195, 199, 202, 208, 210, 211, 213, 214, 216, 217, 220, 222–224, 228, 230–232, 234, 235, 244, 246, 249, 252–256, 258, 260–263, 266, 267, 270]SOX-51 (1%)[204]TGF-β332 (29%)[51, 162, 164, 168, 177, 181–184, 197, 200, 205–207, 218, 223–225, 227, 237, 239, 240, 245, 247, 248, 250, 251, 257, 259, 267, 268, 270]SOX-61 (1%)[204]BMP-213 (12%)[188, 202, 213, 219, 225–227, 229, 264, 265, 267, 270, 271]WNT3A1 (1%)…”

Section: Resultsmentioning

confidence: 99%

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

et al. 2017

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BackgroundThe management of articular cartilage defects presents many clinical challenges due to its avascular, aneural and alymphatic nature. Bone marrow stimulation techniques, such as microfracture, are the most frequently used method in clinical practice however the resulting mixed fibrocartilage tissue which is inferior to native hyaline cartilage. Other methods have shown promise but are far from perfect. There is an unmet need and growing interest in regenerative medicine and tissue engineering to improve the outcome for patients requiring cartilage repair. Many published reviews on cartilage repair only list human clinical trials, underestimating the wealth of basic sciences and animal studies that are precursors to future research. We therefore set out to perform a systematic review of the literature to assess the translation of stem cell therapy to explore what research had been carried out at each of the stages of translation from bench-top (in vitro), animal (pre-clinical) and human studies (clinical) and assemble an evidence-based cascade for the responsible introduction of stem cell therapy for cartilage defects.Main body of abstractThis review was conducted in accordance to PRISMA guidelines using CINHAL, MEDLINE, EMBASE, Scopus and Web of Knowledge databases from 1st January 1900 to 30th June 2015. In total, there were 2880 studies identified of which 252 studies were included for analysis (100 articles for in vitro studies, 111 studies for animal studies; and 31 studies for human studies). There was a huge variance in cell source in pre-clinical studies both of terms of animal used, location of harvest (fat, marrow, blood or synovium) and allogeneicity. The use of scaffolds, growth factors, number of cell passages and number of cells used was hugely heterogeneous.Short conclusionsThis review offers a comprehensive assessment of the evidence behind the translation of basic science to the clinical practice of cartilage repair. It has revealed a lack of connectivity between the in vitro, pre-clinical and human data and a patchwork quilt of synergistic evidence. Drivers for progress in this space are largely driven by patient demand, surgeon inquisition and a regulatory framework that is learning at the same pace as new developments take place.

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“…As an important class of compounds, Schiff bases have received much attention in the wide variety of fields due to structural varieties and unique characteristics such as preparative accessibility, varied coordination ability, thermal stability, biological activities and catalysis properties [8][9][10]. Schiff base ligands are able to coordinate with many different metals and stabilize them in various oxidation states [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Formation constants and thermodynamic parameters of bivalent Co, Ni, Cu and Zn complexes with Schiff base ligand: Experimental and DFT calculations

Esmaielzadeh¹,

Mashhadiagha²

2017

Bull. Chem. Soc. Eth.

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ABSTRACT. Formation constant (Kf) values of Schiff base ligand, L = methyl-2-{[1-methyl-2-(aceton)ethylidynenitrilo]ethyl}amino-1-cyclopentenedithiocarboxylate, with Co(II), Ni(II), Cu(II) and Zn(II) ions has been determined spectrophotometrically for 1:1 complex formation at constant ionic strength 0.01 M (NaClO4), and at various temperatures in DMF solvent. Thermodynamic studies of the complexes have been carried out and their stability were found in the order CoL > CuL > NiL > ZnL. The enthalpy, entropy and Gibbs free energy changes of the complexation reaction have been evaluated from the temperature dependence of the formation constant. Our experimental results revealed that the complexation process is spontaneous, exothermic and entropically unfavorable. Also, B3LYP/(LANL2DZ and ‫)٭٭‪6-311G‬‬ level density functional theory is applied on the structure and stability of Schiff base ligand and their complexes. HOMO-LUMO and binding energies were calculated to obtained stability of the complexes. The theoretical results are compared with experimental data.

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Engineered Microtissues Formed by Schiff Base Crosslinking Restore the Chondrogenic Potential of Aged Mesenchymal Stem Cells

Cited by 25 publications

References 55 publications

Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair

Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

Formation constants and thermodynamic parameters of bivalent Co, Ni, Cu and Zn complexes with Schiff base ligand: Experimental and DFT calculations

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