Engineered living hydrogels for robust biocatalysis in pure organic solvents

Gao, Liang; Feng, Lilin; Sauer, Daniel F.; Wittwer, Malte; Hu, Yong; Schiffels, Johannes; Li, Xin

doi:10.1016/j.xcrp.2022.101054

Cited by 9 publications

(8 citation statements)

References 43 publications

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“…Recent studies have shown that nanocarriers have emerged as a novel toolbox for further improving the bioavailability and delivery efficiency of drugs as well as alleviating their toxicity of drugs during intravenous injection [ 59 – 62 ]. A variety of functionalized nanocarriers have been designed to improve drug solubility and stability, prolong blood circulation, augment targeted accumulation, elevate tumor penetration, and control drug release (Fig.…”

Section: Icaritin-based Nanomedicines For Improved Immunotherapy Of A...mentioning

confidence: 99%

Nanomedicine‐boosting icaritin-based immunotherapy of advanced hepatocellular carcinoma

Gao

Yang

et al. 2022

Military Med Res

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Traditional treatments for advanced hepatocellular carcinoma (HCC), such as surgical resection, transplantation, radiofrequency ablation, and chemotherapy are unsatisfactory, and therefore the exploration of powerful therapeutic strategies is urgently needed. Immunotherapy has emerged as a promising strategy for advanced HCC treatment due to its minimal side effects and long-lasting therapeutic memory effects. Recent studies have demonstrated that icaritin could serve as an immunomodulator for effective immunotherapy of advanced HCC. Encouragingly, in 2022, icaritin soft capsules were approved by the National Medical Products Administration (NMPA) of China for the immunotherapy of advanced HCC. However, the therapeutic efficacy of icaritin in clinical practice is impaired by its poor bioavailability and unfavorable in vivo delivery efficiency. Recently, functionalized drug delivery systems including stimuli-responsive nanocarriers, cell membrane-coated nanocarriers, and living cell-nanocarrier systems have been designed to overcome the shortcomings of drugs, including the low bioavailability and limited delivery efficiency as well as side effects. Taken together, the development of icaritin-based nanomedicines is expected to further improve the immunotherapy of advanced HCC. Herein, we compared the different preparation methods for icaritin, interpreted the HCC immune microenvironment and the mechanisms underlying icaritin for treatment of advanced HCC, and discussed both the design of icaritin-based nanomedicines with high icaritin loading and the latest progress in icaritin-based nanomedicines for advanced HCC immunotherapy. Finally, the prospects to promote further clinical translation of icaritin-based nanomedicines for the immunotherapy of advanced HCC were proposed.

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Section: Icaritin-based Nanomedicines For Improved Immunotherapy Of A...mentioning

confidence: 99%

Nanomedicine‐boosting icaritin-based immunotherapy of advanced hepatocellular carcinoma

Gao

Yang

et al. 2022

Military Med Res

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“…These bacteria had been embedded in alginate hydrogel, compatible with organic solvent tolerance hydrogel. The results demonstrated the successfully engineered living hydrogel, which has shown the biocatalytic activity to catalyze organic solvents 51 . PLA and DA copolymer had been synthesized to encapsulate engineered bacteria ( E. coli ) for L‐lactate biosensors.…”

Section: Application Of Polymeric Film and Hydrogelmentioning

confidence: 90%

Impact of polymeric films and hydrogels: Physical characteristics on bacterial growth

Techakasikornpanich,

Jangpatarapongsa,

Polpanich

et al. 2024

Polymers for Advanced Techs

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Hydrogels find diverse applications in manipulating bacteria, and serving purposes like elevation, maintenance, and elimination. Several factors of hydrogel have been studied in the benefits of antibacterial activity. Factors such as hydrogel stiffness and roughness gain significance in surface coating, influencing bacterial behavior. However, the intricate interplay of hydrogel stiffness, roughness, polymer types, and bacterial species necessitates further exploration. The choice of polymer is dictated by the specific objectives, particularly in antibacterial scenarios where polymers with positive charge, hydrophilicity, and acidity prove effective. These properties induce robust electrostatic and hydrophobic/hydrophilic interactions, along with pH‐induced cell membrane damage, collectively contributing to hindered bacterial adhesion and growth. Additionally, extracellular polymeric substances (EPS) emerge as pivotal influencers in bacterial adhesion and proliferation. EPS production alters bacterial surfaces, fostering connections between bacteria and facilitating biofilm formation. The hydrophobic nature of EPS further complicates bacterial interactions with surface materials, emphasizing the nuanced interplay of hydrophilic and hydrophobic forces in bacterial adhesion. Herein, this work article has reviewed the related study of each physical property related to antibacterial property on the surface of the hydrogel. Moreover, this work also illustrates applications of the antibacterial properties of hydrogel for medical and surface treatment, including wound healing, food packaging, and surface coating. Additionally, the bacteria growing on hydrogel for engineered living materials, have been updated in various applications.

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“…ELMs have the potential to replace many conventional materials used in construction, civil infrastructure, and bioremediation. [1][2][3][4] Despite enthusiasm for ELMs, a major challenge to the development of ELMs is maintaining the viability of living cells within the material. Existing ELMs do not include methods of delivering nutrients to resident cells, limiting the service life of the living component to hours.…”

Section: Introductionmentioning

confidence: 99%

“…The most successful demonstrations of ELMs have used hydrogels which are porous enough to enable diffusion of nutrients and waste products to and from embedded cells. [2,3,5] Organisms within the hydrogel can enable controlled secretion of proteins to assemble structures, [6] biodegradability, [7] high stretchability, [8] and biocompatibility for tissue engineering. [6][7][8][9] In all demonstrations to date, functionalities provided by the living components of ELMs have been limited by the viability of resident cells, which run out of nutrients or accumulate excessive metabolic byproducts within hours of activity.…”

Section: Introductionmentioning

confidence: 99%

Solute Transport in Engineered Living Materials Using Bone‐Inspired Microscale Channel Networks

van Wijngaarden,

Bratcher,

Lewis

et al. 2023

Adv Eng Mater

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Engineered living materials (ELMs) are an emerging class of materials that are synthesized and/or populated by living cells to achieve novel functionalities including self‐healing and sensing. Providing nutrients to living cells within an ELM over prolonged periods remains a major technical challenge that limits the service life of ELMs. Bone maintains living cells for decades by delivering nutrients through a network of nanoscale channels punctuated by microscale pores. Nutrient transfer in bone is enabled by mechanical loading experienced by the material during regular use. Here we identify the geometric traits of the network of channels and pores that can be used in engineered living materials to allow mechanical loading to enable nutrient delivery to resident cell populations in a manner seen in bone. Transport occurs when deformation in the microscale pore network exceeds the volume of the connecting channels. Computational models show that transport is enhanced at greater loading magnitudes and lower loading frequencies. The computational results are confirmed using experiments with microfluidic systems. Our findings provide quantitative design principles for channel‐pore networks capable of sustained delivery of nutrients to living cells within materials.This article is protected by copyright. All rights reserved.

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Engineered living hydrogels for robust biocatalysis in pure organic solvents

Cited by 9 publications

References 43 publications

Nanomedicine‐boosting icaritin-based immunotherapy of advanced hepatocellular carcinoma

Nanomedicine‐boosting icaritin-based immunotherapy of advanced hepatocellular carcinoma

Impact of polymeric films and hydrogels: Physical characteristics on bacterial growth

Solute Transport in Engineered Living Materials Using Bone‐Inspired Microscale Channel Networks

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